Actin Cytoskeleton Remodeling Accompanied by Redistribution of Adhesion Proteins Drives Migration of Cells in Different EMT States
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines, Constructs, and Transfections
2.2. Antibodies and Reagents
2.3. Immunofluorescence
2.4. Live-Cell Imaging, Tracking, and Kymograph Analysis
2.5. Co-Immunoprecipitation and Western Blotting
3. Results
3.1. EGF-Induced EMT of IAR-20 Epithelial Cells
3.2. Migratory Activity of IAR-6-1 Cells Exhibiting Hybrid Epithelial–Mesenchymal Phenotype and Their Descendant IAR-6-1-DNE Cells
3.3. Redistribution of α-Catenin in Cells in Different EMT States
3.4. α-Catenin Is Involved in Cell Migration
3.5. pY654-β-Catenin Enrichment at Integrin Adhesion Complexes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ilnitskaya, A.S.; Litovka, N.I.; Rubtsova, S.N.; Zhitnyak, I.Y.; Gloushankova, N.A. Actin Cytoskeleton Remodeling Accompanied by Redistribution of Adhesion Proteins Drives Migration of Cells in Different EMT States. Cells 2024, 13, 780. https://doi.org/10.3390/cells13090780
Ilnitskaya AS, Litovka NI, Rubtsova SN, Zhitnyak IY, Gloushankova NA. Actin Cytoskeleton Remodeling Accompanied by Redistribution of Adhesion Proteins Drives Migration of Cells in Different EMT States. Cells. 2024; 13(9):780. https://doi.org/10.3390/cells13090780
Chicago/Turabian StyleIlnitskaya, Alla S., Nikita I. Litovka, Svetlana N. Rubtsova, Irina Y. Zhitnyak, and Natalya A. Gloushankova. 2024. "Actin Cytoskeleton Remodeling Accompanied by Redistribution of Adhesion Proteins Drives Migration of Cells in Different EMT States" Cells 13, no. 9: 780. https://doi.org/10.3390/cells13090780