MicroRNA-21 Mediates the Protective Effect of Cardiomyocyte-Derived Conditioned Medium on Ameliorating Myocardial Infarction in Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics
2.2. Animals and Induction of Acute Myocardial Infarction in Rats
2.3. Culturing of Cardiac Cells
2.4. Collection of Exosomes
2.5. Quantitative Reverse Transcription Polymerase Chain Reaction
2.6. Histopathological and Immunostaining
2.7. Western Blot
2.8. Statistical Analysis
3. Results
3.1. Oxygen–Glucose Deprivation Regulates microRNA Expression Profiles in Cardiac Cells
3.2. Inhibition of miR-21 Reduced the Protective Effect of Cardiomyocyte-Derived Conditioned Medium Cardiomyocytes Against Oxidative Stress
3.3. Inhibition of miR-21 Reduced the Effect of Conditioned Medium on Activation of Cardiac Fibroblasts
3.4. Inhibition of miR-21 Reduced the Effect of Conditioned Medium on Angiogenic Enhancement in Endothelial Cells
3.5. Inhibition of miR-21 Reduced the Effect of Conditioned Medium on Ameliorating Myocardial Injury in Rats with Acute Myocardial Infarction
3.6. Inhibition of miR-21 Reduced the Effect of Conditioned Medium on Reducing Immune Cell Infiltration in Myocardium with Acute Myocardial Infarction in Rats
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Chen, C.-H.; Hsu, S.-Y.; Chiu, C.-C.; Leu, S. MicroRNA-21 Mediates the Protective Effect of Cardiomyocyte-Derived Conditioned Medium on Ameliorating Myocardial Infarction in Rats. Cells 2019, 8, 935. https://doi.org/10.3390/cells8080935
Chen C-H, Hsu S-Y, Chiu C-C, Leu S. MicroRNA-21 Mediates the Protective Effect of Cardiomyocyte-Derived Conditioned Medium on Ameliorating Myocardial Infarction in Rats. Cells. 2019; 8(8):935. https://doi.org/10.3390/cells8080935
Chicago/Turabian StyleChen, Chih-Hung, Shu-Yuan Hsu, Chien-Chih Chiu, and Steve Leu. 2019. "MicroRNA-21 Mediates the Protective Effect of Cardiomyocyte-Derived Conditioned Medium on Ameliorating Myocardial Infarction in Rats" Cells 8, no. 8: 935. https://doi.org/10.3390/cells8080935