Aspirin Mitigated Tumor Growth in Obese Mice Involving Metabolic Inhibition
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Cell Viability Assay
2.3. Caspase 3 Activity Assay
2.4. Syngeneic Tumor Model Study
2.5. Colony Formation Assay
2.6. Blood Sample Collection and Analyses
2.7. Western Blot
2.8. Statistical Analysis
3. Results
3.1. Tumor Improved Glucose Metabolism in Obese Mice
3.2. Tumor Elevated Circulating Leukocytes in Obese Mice
3.3. Aspirin Improved Metabolic and Inflammatory Alterations in Tumor-Bearing HFD-Fed Mice
3.4. Aspirin Mitigated Cellular Metabolic Capacity and Cell Growth in Tumor-Bearing HFD-Fed Mice
3.5. Aspirin Mitigated LLC Cell Proliferation and Metabolism
3.6. Glutamine Promoted LLC Cell Proliferation and Metabolism
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Wang, J.-D.; Chen, W.-Y.; Li, J.-R.; Lin, S.-Y.; Wang, Y.-Y.; Wu, C.-C.; Liao, S.-L.; Ko, C.-C.; Chen, C.-J. Aspirin Mitigated Tumor Growth in Obese Mice Involving Metabolic Inhibition. Cells 2020, 9, 569. https://doi.org/10.3390/cells9030569
Wang J-D, Chen W-Y, Li J-R, Lin S-Y, Wang Y-Y, Wu C-C, Liao S-L, Ko C-C, Chen C-J. Aspirin Mitigated Tumor Growth in Obese Mice Involving Metabolic Inhibition. Cells. 2020; 9(3):569. https://doi.org/10.3390/cells9030569
Chicago/Turabian StyleWang, Jiaan-Der, Wen-Ying Chen, Jian-Ri Li, Shih-Yi Lin, Ya-Yu Wang, Chih-Cheng Wu, Su-Lan Liao, Chiao-Chen Ko, and Chun-Jung Chen. 2020. "Aspirin Mitigated Tumor Growth in Obese Mice Involving Metabolic Inhibition" Cells 9, no. 3: 569. https://doi.org/10.3390/cells9030569