Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Chilling-Induced Influences on the Volatile Components of R. ‘Crimson Glory’
2.3. DNA Isolation, Library Construction, and WGBS-Seq
2.4. RNA-Seq and Differentially Expressed Gene (DEG) Identification
2.5. Identification of Differentially Methylated Regions (DMRs) and DMR-Related Genes
2.6. Gene Validation and Expression Analyses
3. Results
3.1. Chilling-Induced Influences on VOCs Emissions
3.2. Characteristics of DNA Methylation in R. ‘Crimson Glory’
3.3. Identification of DNA Methylation and DEGs
3.4. Identification of DEGs and DMRs Associated with Volatile Biosynthesis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xie, L.; Bai, X.; Zhang, H.; Qiu, X.; Jian, H.; Wang, Q.; Wang, H.; Feng, D.; Tang, K.; Yan, H. Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis. Genes 2023, 14, 692. https://doi.org/10.3390/genes14030692
Xie L, Bai X, Zhang H, Qiu X, Jian H, Wang Q, Wang H, Feng D, Tang K, Yan H. Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis. Genes. 2023; 14(3):692. https://doi.org/10.3390/genes14030692
Chicago/Turabian StyleXie, Limei, Xue Bai, Hao Zhang, Xianqin Qiu, Hongying Jian, Qigang Wang, Huichun Wang, Dedang Feng, Kaixue Tang, and Huijun Yan. 2023. "Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis" Genes 14, no. 3: 692. https://doi.org/10.3390/genes14030692