Next Article in Journal / Special Issue
Clustering Rfam 10.1: Clans, Families, and Classes
Previous Article in Journal
The Human Transcriptome: An Unfinished Story
Previous Article in Special Issue
The Role of Bromodomain Proteins in Regulating Gene Expression
Article Menu

Export Article

Open AccessCommunication
Genes 2012, 3(3), 361-377; doi:10.3390/genes3030361

Genome-Wide Sequencing Reveals Two Major Sub-Lineages in the Genetically Monomorphic Pathogen Xanthomonas Campestris Pathovar Musacearum

1
Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
2
National Crops Resources Research Institute (NaCRRI), Kampala 7084, Uganda
3
The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK
Present address: International Centre for Tropical Agriculture (CIAT), Kawanda Agricultural Research Institute, Kampala 6247, Uganda.
*
Author to whom correspondence should be addressed.
Received: 10 June 2012 / Revised: 24 June 2012 / Accepted: 26 June 2012 / Published: 4 July 2012
(This article belongs to the Special Issue Feature Paper 2012)
View Full-Text   |   Download PDF [691 KB, uploaded 4 July 2012]   |  

Abstract

The bacterium Xanthomonas campestris pathovar musacearum (Xcm) is the causal agent of banana Xanthomonas wilt (BXW). This disease has devastated economies based on banana and plantain crops (Musa species) in East Africa. Here we use genome-wide sequencing to discover a set of single-nucleotide polymorphisms (SNPs) among East African isolates of Xcm. These SNPs have potential as molecular markers for phylogeographic studies of the epidemiology and spread of the pathogen. Our analysis reveals two major sub-lineages of the pathogen, suggesting that the current outbreaks of BXW on Musa species in the region may have more than one introductory event, perhaps from Ethiopia. Also, based on comparisons of genome-wide sequence data from multiple isolates of Xcm and multiple strains of X. vasicola pathovar vasculorum, we identify genes specific to Xcm that could be used to specifically detect Xcm by PCR-based methods. View Full-Text
Keywords: banana Xanthomonas wilt; whole-genome sequencing; SNP; molecular markers; Ensete ventricosum; Xanthomonas vasicola banana Xanthomonas wilt; whole-genome sequencing; SNP; molecular markers; Ensete ventricosum; Xanthomonas vasicola
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary materials

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wasukira, A.; Tayebwa, J.; Thwaites, R.; Paszkiewicz, K.; Aritua, V.; Kubiriba, J.; Smith, J.; Grant, M.; Studholme, D.J. Genome-Wide Sequencing Reveals Two Major Sub-Lineages in the Genetically Monomorphic Pathogen Xanthomonas Campestris Pathovar Musacearum. Genes 2012, 3, 361-377.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top