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Genes 2012, 3(4), 759-778; doi:10.3390/genes3040759

Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily

Department of Molecular Genetics, Weizmann Institute of Science, 76100 Rehovot, Israel
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Received: 27 September 2012 / Revised: 22 October 2012 / Accepted: 31 October 2012 / Published: 21 November 2012
(This article belongs to the Special Issue RNA Interference)
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Abstract

The way we study cortical development has undergone a revolution in the last few years following the ability to use shRNA in the developing brain of the rodent embryo. The first gene to be knocked-down in the developing brain was doublecortin (Dcx). Here we will review knockdown experiments in the developing brain and compare them with knockout experiments, thus highlighting the advantages and disadvantages using the different systems. Our review will focus on experiments relating to the doublecortin superfamily of proteins. View Full-Text
Keywords: doublecortin; DCX; shRNA; in utero electroporation; neuronal migration doublecortin; DCX; shRNA; in utero electroporation; neuronal migration
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Reiner, O.; Gorelik, A.; Greenman, R. Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily. Genes 2012, 3, 759-778.

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