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Genes 2017, 8(8), 195; doi:10.3390/genes8080195

Dissecting Tissue-Specific Transcriptomic Responses from Leaf and Roots under Salt Stress in Petunia hybrida Mitchell

1
School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, USA
2
Institute for Translational Medicine and Therapeutics (ITMAT), University of Pennsylvania, Philadelphia, PA 19104, USA
3
Boyce Thompson Institute, Cornell University, Ithaca, NY 14853, USA
4
Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
*
Author to whom correspondence should be addressed.
Received: 9 April 2017 / Revised: 1 July 2017 / Accepted: 27 July 2017 / Published: 3 August 2017
(This article belongs to the Section Plant Genetics and Genomics)
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Abstract

One of the primary objectives of plant biotechnology is to increase resistance to abiotic stresses, such as salinity. Salinity is a major abiotic stress and increasing crop resistant to salt continues to the present day as a major challenge. Salt stress disturbs cellular environment leading to protein misfolding, affecting normal plant growth and causing agricultural losses worldwide. The advent of state-of-the-art technologies such as high throughput mRNA sequencing (RNA-seq) has revolutionized whole-transcriptome analysis by allowing, with high precision, to measure changes in gene expression. In this work, we used tissue-specific RNA-seq to gain insight into the Petunia hybrida transcriptional responses under NaCl stress using a controlled hydroponic system. Roots and leaves samples were taken from a continuum of 48 h of acute 150 mM NaCl. This analysis revealed a set of tissue and time point specific differentially expressed genes, such as genes related to transport, signal transduction, ion homeostasis as well as novel and undescribed genes, such as Peaxi162Scf00003g04130 and Peaxi162Scf00589g00323 expressed only in roots under salt stress. In this work, we identified early and late expressed genes in response to salt stress while providing a core of differentially express genes across all time points and tissues, including the trehalose-6-phosphate synthase 1 (TPS1), a glycosyltransferase reported in salt tolerance in other species. To test the function of the novel petunia TPS1 allele, we cloned and showed that TPS1 is a functional plant gene capable of complementing the trehalose biosynthesis pathway in a yeast tps1 mutant. The list of candidate genes to enhance salt tolerance provided in this work constitutes a major effort to better understand the detrimental effects of salinity in petunia with direct implications for other economically important Solanaceous species. View Full-Text
Keywords: salinity; salt stress; petunia; RNA-seq transcriptomic; trehalose; yeast complementation salinity; salt stress; petunia; RNA-seq transcriptomic; trehalose; yeast complementation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Villarino, G.H.; Hu, Q.; Scanlon, M.J.; Mueller, L.; Bombarely, A.; Mattson, N.S. Dissecting Tissue-Specific Transcriptomic Responses from Leaf and Roots under Salt Stress in Petunia hybrida Mitchell. Genes 2017, 8, 195.

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