Semen Cryopreservation to Expand Male Fertility in Cancer Patients: Intracase Evaluation of Semen Quality
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient Recruitment
2.2. Semen Quality Assessment
2.2.1. Motility Assessment
2.2.2. Morphology Assessment
2.3. Pre-Freezing and Freezing Procedures
2.4. Statistical Analysis
3. Results
3.1. Epidemiological Data and Clinical Characteristics
3.2. Semen Quality Assessment in Cancer Subgroups
3.3. Comparison and Correlation between Pre- and Post-Cryopreservation Semen Parameters
3.4. Age-Dependent Impact on Semen Quality
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Type of Cancer | ≤15 × 106/mL | >15 × 106/mL | p-Value * |
---|---|---|---|
a Testicular cancer (n = 135) | 70.37% (95) | 29.63% (40) | 0.003 (a vs. b) |
b Hematol. cancers (n = 76) | 50.0% (38) | 50.0% (38) | 0.06 (a vs. c) |
c Other cancers (n = 43) | 55.8% (24) | 44.2% (19) | 0.0028 (a vs. b + c) |
Whole Cohort | Hematol. Cancers | Other Cancers | Testicular Cancer | p-Value | WHO 2021 | |
---|---|---|---|---|---|---|
Age, mean ± SD | 30.2 ± 7.6 | 28.4 ± 8.5 | 31.9 ± 9.2 | 30.7 ± 6.1 | 0.02 a n.s. b | - - |
Ejaculate volume, mL | 2.2 ± 1.3 | 2.03 ± 1.2 | 2.48 ± 1.5 | 2.2 ± 1.3 | n.s. a n.s. b | 1.4 (1.3–1.5) |
Number/mL, ×106 | 21.2 ± 28.7 | 26.9 ± 28.3 | 29.6 ± 39.9 | 15.3 ± 23.0 | 0.0014 a 0.004 b | 39 (35–40) |
Total motility, % | 18.2 ± 17.8 | 21.7 ± 18.6 | 19.1 ± 20 | 16.0 ± 16.3 | 0.02 a 0.07 b | 42 (40–43) |
Progressive motility, % | 6.6 ± 8.9 | 8.02 ± 9.4 | 7.1 ± 10.6 | 5.7 ± 7.9 | 0.04 a 0.05 b | 30 (29–31) |
Viability, % | 62.3 ± 18.7 | 67.1 ± 16.3 | 59.4 ± 18.4 | 60.3 ± 19.9 | 0.01 a n.s. b | 54 (50–56) |
Morphology, % | 7.73 ± 8.4 | 8.75 ± 8.7 | 9.56 ± 9.8 | 6.59 ± 7.5 | 0.05 a 0.03 b | 4 (3.9–4) |
Pre-Cryopreservation | ||||
---|---|---|---|---|
Post-Cryopreservation | Viability (%) | Progressive Motility (%) | ||
Whole cohort | Viability, % | R2 = 0.35; p < 0.001 | R2 = 0.42; p < 0.001 | |
Progressive motility, % | R2 = 0.35; p < 0.001 | R2 = 0.45; p < 0.001 | ||
Hematological cancers (HC) | Viability, % | R2 = 0.12; p = n.s. | R2 = 0.16; p = n.s. | |
Progressive motility, % | R2 = 0.18; p = n.s. | R2 = 0.13; p = n.s. | ||
Other cancers (OC) | Viability, % | R2 = 0.09; p = n.s | R2 = 0.06; p = n.s | |
Progressive motility, % | R2 = 0.08; p = n.s | R2 = 0.07; p = n.s | ||
Testis cancer (TC) | Viability, % | R2 = 0.2; p < 0.05 | R2 = 0.18; p < 0.05 | |
Progressive motility, % | R2 = 0.16; p < 0.05 | R2 = 0.2; p < 0.05 |
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Peluso, G.; Tisato, V.; Singh, A.V.; Gemmati, D.; Scarpellini, F. Semen Cryopreservation to Expand Male Fertility in Cancer Patients: Intracase Evaluation of Semen Quality. J. Pers. Med. 2023, 13, 1654. https://doi.org/10.3390/jpm13121654
Peluso G, Tisato V, Singh AV, Gemmati D, Scarpellini F. Semen Cryopreservation to Expand Male Fertility in Cancer Patients: Intracase Evaluation of Semen Quality. Journal of Personalized Medicine. 2023; 13(12):1654. https://doi.org/10.3390/jpm13121654
Chicago/Turabian StylePeluso, Giuseppina, Veronica Tisato, Ajay Vikram Singh, Donato Gemmati, and Fabio Scarpellini. 2023. "Semen Cryopreservation to Expand Male Fertility in Cancer Patients: Intracase Evaluation of Semen Quality" Journal of Personalized Medicine 13, no. 12: 1654. https://doi.org/10.3390/jpm13121654