Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated Tuta absoluta
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insect Breeding
2.2. Sample Preparation and DNA Extraction
2.3. 16S rRNA Gene Amplification and Sequencing
2.4. 16S rRNA-Based Bacterial Community Analysis
3. Results
3.1. Sequencing Data
3.2. Microbiomes of Virgin Tomato Leafminer
3.3. Mating-Induced Changes in Genital Microbiome
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sample | Organ | Sex | Mating Status | Total Number |
---|---|---|---|---|
IRF0 | Internal Reproductive System | Female | Virgin | 20 × 5 |
IRM0 | Internal Reproductive System | Male | Virgin | 20 × 5 |
IRF1 | Internal Reproductive System | Female | Mated | 20 × 5 |
IRM1 | Internal Reproductive System | Male | Mated | 20 × 5 |
DF0 | Digestive tract | Female | Virgin | 20 × 5 |
DM0 | Digestive tract | Male | Virgin | 20 × 5 |
DF1 | Digestive tract | Female | Mated | 20 × 5 |
DM1 | Digestive tract | Male | Mated | 20 × 5 |
Egg | - | - | - | 200 × 5 |
ID | IRF0 | IRM0 | IRF1 | IRM1 |
---|---|---|---|---|
Wolbachia endosymbiont of Bryobia spec. I VIDR-2008 | 68.73 ± 5.66% | 67.34 ± 8.03% | 81.43 ± 2.24% | 82.76 ± 3.25% |
Burkholderia cenocepacia | 5.05 ± 1.12% | 14.29 ± 4.40% | 1.54 ± 0.39% | 10.19 ± 3.12% |
Ralstonia sp. 1F2 | 8.93 ± 2.55% | 12.19 ± 2.43% | 4.47 ± 1.19% | 3.92 ± 0.85% |
Sediminibacterium salmoneum | 4.73 ± 0.91% | 3.90 ± 1.11% | 0.69 ± 0.15% | 0.79 ± 0.31% |
Enterobacter cancerogenus | 2.02 ± 0.80% | 0.23 ± 0.19% | 4.22 ± 1.25% | 0.36 ± 0.16% |
Enterobacter asburiae | 1.40 ± 0.32% | 0.08 ± 0.03% | 2.50 ± 0.67% | 0.13 ± 0.07% |
Enterobacter kobei | 1.04 ± 0.59% | 0.00% | 2.23 ± 1.18% | 0.15 ± 0.06% |
Caulobacter sp. | 1.23 ± 0.37% | 0.67 ± 0.05% | 0.23 ± 0.07% | 1.02 ± 0.32% |
Enterobacter hormaechei | 2.60 ± 1.69% | 0.00% | 0.00% | 0.00% |
Enterobacter cloacae | 1.82 ± 1.15% | 0.00% | 0.00% | 0.00% |
Pantoea sp. NJ-32 | 0.43 ± 0.25% | 0.00% | 1.10 ± 0.39% | 0.00% |
Alphaproteobacteria bacterium | 0.51 ± 0.14% | 0.47 ± 0.16% | 0.26 ± 0.10% | 0.22 ± 0.13% |
Enterobacter sp. CTSP4 | 0.32 ± 0.19% | 0.00% | 0.85 ± 0.45% | 0.00% |
Microbacterium sp. Sw0106-31(2) | 0.15 ± 0.07% | 0.06 ± 0.02% | 0.28 ± 0.19% | 0.00% |
Afipia sp. BAC308 | 0.10 ± 0.03% | 0.00% | 0.00% | 0.00% |
alpha proteobacterium PII-14 | 0.00% | 0.00% | 0.00% | 0.04 ± 0.02% |
Others | 0.57 ± 0.46% | 0.06 ± 0.04% | 0.12 ± 0.06% | 0.09 ± 0.05% |
ID | DF0 | DM0 | DF1 | DM1 |
---|---|---|---|---|
Enterobacter cancerogenus | 32.79 ± 2.24% | 21.34 ± 1.09% | 30.72 ± 1.95% | 33.50 ± 1.89% |
Enterobacter asburiae | 26.93 ± 3.44% | 23.94 ± 6.44% | 18.67 ± 2.23% | 19.43 ± 1.76% |
Enterobacter kobei | 12.61 ± 3.29% | 7.82 ± 1.59% | 16.18 ± 2.63% | 17.40 ± 1.63% |
Wolbachia endosymbiont of Bryobia spec. I VIDR-2008 | 4.01 ± 1.34% | 13.35 ± 4.67% | 14.34 ± 3.11% | 11.94 ± 3.36% |
Burkholderia cenocepacia | 7.82 ± 4.39% | 8.22 ± 2.75% | 3.43 ± 0.94% | 2.70 ± 0.87% |
Pantoea sp. NJ-32 | 5.19 ± 1.55% | 2.84 ± 0.48% | 6.67 ± 0.74% | 6.41 ± 0.61% |
Enterobacter sp. CTSP4 | 4.83 ± 1.31% | 2.59 ± 0.64% | 5.20 ± 0.68% | 5.16 ± 0.25% |
Ralstonia sp. 1F2 | 1.47 ± 0.84% | 1.05 ± 0.43% | 2.07 ± 0.50% | 0.64 ± 0.29% |
Klebsiella aerogenes | 0.64 ± 0.27% | 0.00% | 0.82 ± 0.50% | 0.97 ± 0.36% |
Enterobacter cloacae | 1.03 ± 0.67% | 0.00% | 0.00% | 0.00% |
Caulobacter sp. | 0.21 ± 0.08% | 0.55 ± 0.20% | 0.49 ± 0.15% | 0.29 ± 0.14% |
Sediminibacterium salmoneum | 0.17 ± 0.11% | 0.68 ± 0.36% | 0.18 ± 0.04% | 0.11 ± 0.03% |
Alphaproteobacteria bacterium | 0.00% | 0.46 ± 0.36% | 0.00% | 0.11 ± 0.05% |
Others | 0.23 ± 0.10% | 0.04 ± 0.02% | 0.32 ± 0.24% | 0.20 ± 0.12% |
ID | E |
---|---|
Wolbachia endosymbiont of Bryobia spec. I VIDR-2008 | 39.76 ± 7.85% |
Burkholderia cenocepacia | 27.86 ± 3.78% |
Solanum violaceimarmoratum | 4.82 ± 1.84% |
Caulobacter sp. | 2.88 ± 0.48% |
Enterobacter asburiae | 1.32 ± 1.01% |
Janibacter-like sp. V4.BO.43 | 0.93 ± 0.75% |
Solanum pennellii | 0.92 ± 0.27% |
Caulobacter sp. FWC26 | 0.76 ± 0.48% |
Asticcacaulis excentricus | 0.67 ± 0.32% |
Arthrobacter sp. KAR53 | 0.66 ± 0.56% |
Arthrobacter sp. JSM 101049 | 0.56 ± 0.31% |
Deinococcus petrolearius | 0.51 ± 0.42% |
Enterobacter cancerogenus | 0.44 ± 0.11% |
Staphylococcus warneri | 0.40 ± 0.20% |
[Empedobacter] haloabium | 0.37 ± 0.16% |
Others | 13.03 ± 2.48% |
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Bi, S.; Wang, X.; Tang, Y.; Lei, K.; Guo, J.; Yang, N.; Wan, F.; Lü, Z.; Liu, W. Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated Tuta absoluta. Insects 2023, 14, 779. https://doi.org/10.3390/insects14100779
Bi S, Wang X, Tang Y, Lei K, Guo J, Yang N, Wan F, Lü Z, Liu W. Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated Tuta absoluta. Insects. 2023; 14(10):779. https://doi.org/10.3390/insects14100779
Chicago/Turabian StyleBi, Siyan, Xiaodi Wang, Yanhong Tang, Kexin Lei, Jianyang Guo, Nianwan Yang, Fanghao Wan, Zhichuang Lü, and Wanxue Liu. 2023. "Bacterial Communities of the Internal Reproductive and Digestive Tracts of Virgin and Mated Tuta absoluta" Insects 14, no. 10: 779. https://doi.org/10.3390/insects14100779