Short Communication: Latent Detection of Downy Mildew (Peronospora pisi) in Bioassays against Pisum sativum
Abstract
:1. Introduction
2. Results
2.1. Fresh Sporangia Assays
2.2. Soil Experiments
3. Discussion
4. Materials and Methods
4.1. Inoculum Collection and Preparation
4.1.1. Sporangia
4.1.2. Soil
4.2. Experimental Design
4.2.1. Fresh Sporangial Inoculation Experiments
4.2.2. Soil Inoculum Experiments
4.3. Molecular Detection of Peronospora pisi
4.3.1. DNA Extraction
4.3.2. Two-Step Nested PCR
4.3.3. Visualization and Identity Confirmation
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Experiment | Initial N | Inoculum Type | Survived Trial Period | Expressed Signs of Disease | Asymptomatic Infection Detected by PCR |
---|---|---|---|---|---|
1 | 10 | Sporangia | 9 | 0 | 3 |
2 | 10 | Sporangia | 10 | 0 | 7 |
3 | 6 | Sporangia | 1 | 0 | 1 |
4 | 6 | Sporangia | 5 | 0 | 5 |
5 | 50 | Soil | 15 | 0 | 7 |
6 | 93 | Soil | 68 | 2 | 1 |
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Marr, A.; Casonato, S.; Jones, E. Short Communication: Latent Detection of Downy Mildew (Peronospora pisi) in Bioassays against Pisum sativum. Pathogens 2021, 10, 1312. https://doi.org/10.3390/pathogens10101312
Marr A, Casonato S, Jones E. Short Communication: Latent Detection of Downy Mildew (Peronospora pisi) in Bioassays against Pisum sativum. Pathogens. 2021; 10(10):1312. https://doi.org/10.3390/pathogens10101312
Chicago/Turabian StyleMarr, Alexia, Seona Casonato, and Eirian Jones. 2021. "Short Communication: Latent Detection of Downy Mildew (Peronospora pisi) in Bioassays against Pisum sativum" Pathogens 10, no. 10: 1312. https://doi.org/10.3390/pathogens10101312