Whole Blood versus Plasma Samples—How Does the Type of Specimen Collected for Testing Affect the Monitoring of Cytomegalovirus Viremia?
Abstract
:1. Introduction
2. Materials and Methods
2.1. Clinical Samples
2.2. DNA Extraction
2.3. Nucleic Acid Amplification Test
2.4. Data Interpretation and Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Age Group | Sex | n | Initial Hospitalisation Reason |
---|---|---|---|
Children | Female | 11 | Oncology and/or hematology |
Male | 12 | ||
Adults | Female | 15 | Solid organ transplantation |
Male | 15 |
Whole Blood | Plasma | ||
---|---|---|---|
Positive CMV DNA | Positive CMV DNA | Negative CMV DNA | |
n | 156 | 97 | 59 |
% | 100 | 62.2 | 37.8 |
Patient No. | CMV DNA [IU/mL] | |
---|---|---|
in Whole Blood | in Plasma | |
1. | 2870 | 4155 |
2. | 1530 | 5000 |
3. | 880 | 2520 |
4. | 475 | 3253 |
5. | 3180 | 5850 |
3100 | 3240 | |
5850 | 8500 | |
1970 | 3040 | |
6. | 1360 | 4325 |
7. | 9000 | 18,450 |
8. | 123 | 1055 |
9. | 172,670 | 188,000 |
1950 | 2365 | |
10. | 1290 | 10,900 |
1320 | 6550 | |
4613 | 8700 | |
11. | 1,013,340 | 1,585,000 |
11,070 | 17,450 | |
12. | 27,270 | 27,300 |
13. | 45,270 | 88,500 |
Statistical Analysis | Statistically Significance | |
---|---|---|
Correlation of negative plasma result vs. | ||
| χ² = 0.28, p = 0.5945 | No |
| χ² = 0.10, p = 0.7550 | No |
Correlation of the number of CMV load in the whole blood vs. | Yes | |
| Z = 8.2276, p < 0.001 | |
Correlation of CMV load in samples positive in both specimens, whole blood and plasma | Spearman’s r = 0.67, p < 0.001 | Yes |
Correlation of higher value of CMV load in plasma vs. | ||
| χ² = 0.62, p = 0.4309 | No |
| χ² = 0.51, p = 0.4768 | No |
Median DNAemia values for: | ||
| 4260 IU/mL | |
| 1105 IU/mL |
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Rzepka, M.; Depka, D.; Gospodarek-Komkowska, E.; Bogiel, T. Whole Blood versus Plasma Samples—How Does the Type of Specimen Collected for Testing Affect the Monitoring of Cytomegalovirus Viremia? Pathogens 2022, 11, 1384. https://doi.org/10.3390/pathogens11111384
Rzepka M, Depka D, Gospodarek-Komkowska E, Bogiel T. Whole Blood versus Plasma Samples—How Does the Type of Specimen Collected for Testing Affect the Monitoring of Cytomegalovirus Viremia? Pathogens. 2022; 11(11):1384. https://doi.org/10.3390/pathogens11111384
Chicago/Turabian StyleRzepka, Mateusz, Dagmara Depka, Eugenia Gospodarek-Komkowska, and Tomasz Bogiel. 2022. "Whole Blood versus Plasma Samples—How Does the Type of Specimen Collected for Testing Affect the Monitoring of Cytomegalovirus Viremia?" Pathogens 11, no. 11: 1384. https://doi.org/10.3390/pathogens11111384