Genomes of Endotrypanum monterogeii from Panama and Zelonia costaricensis from Brazil: Expansion of Multigene Families in Leishmaniinae Parasites That Are Close Relatives of Leishmania spp.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Origin, Genome Sequencing, and Assembly
2.2. Gene Models, Repetitive Elements, and RNAs Prediction
2.3. Functional Annotation of Genes
2.4. Orthologous Group Analysis
2.5. Datasets for Phylogenomic Analysis
2.6. Phylogenomic Analysis of the Subfamily Leishmaniinae
2.7. Characterization and Phylogenetic Analysis in gp63 Multigene Family
2.8. Metabolic Pathway Prediction
3. Results
3.1. Assembly and Genome Characteristics
3.2. Gene Prediction and Annotation
3.3. Ortholog Clustering and Functional Analysis
3.4. Phylogenomics Analysis into the Subfamily Leishmaniinae
3.5. Phylogenetic Analysis of the GP63 Family
3.6. Metabolic Pathway Comparison between L. (L.) major, Z. costaricensis, and E. monterogeii
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Assembly Features | E. monterogeii M907 | Z. costaricensis TCC169E |
---|---|---|
Number of contigs | 10,088 | 7896 |
Longest contigs (bp) | 41,459 | 126,447 |
N50 length (bp) | 6007 | 17,448 |
L50 | 1445 | 614 |
GC% | 52.66 | 64.26 |
Total length (Mb) | 29.66 | 38.8 |
BUSCO v. 5.2.2 | ||
Complete single-copy | 125 | 129 |
Complete single duplicated | 0 | 0 |
Fragmented | 5 | 1 |
Missing | 0 | 0 |
Features | E. monterogeii | Percentage | Z. costaricensis | Percentage |
---|---|---|---|---|
Protein-coding genes | 9711 | NA | 12,201 | NA |
G + C% of CDS | 57.12 | NA | 68.39 | NA |
Mean exon length (bp) | 1580 | NA | 1573 | NA |
Nr-DB * | 8834 | 90.90% | 11,380 | 93.30% |
KAAS-KEGG * | 2088 | 21.50% | 2344 | 19.20% |
Pfam-DB * | 7241 | 74.60% | 8975 | 73.50% |
EggNOG-DB * | 7738 | 79.60% | 9173 | 75.20% |
Interproscan * | 8497 | 87.50% | 11,171 | 95.50% |
Types | Z. costaricensis | E. monterogeii | Software |
---|---|---|---|
rRNA | 8 | 6 | RNAmmer |
tRNA | 201 | 295 | tRNAscan |
microRNA | 295 | 307 | Infernal |
snoRNA | 95 | 213 | Infernal |
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Tullume-Vergara, P.O.; Caicedo, K.Y.O.; Tantalean, J.F.C.; Serrano, M.G.; Buck, G.A.; Teixeira, M.M.G.; Shaw, J.J.; Alves, J.M.P. Genomes of Endotrypanum monterogeii from Panama and Zelonia costaricensis from Brazil: Expansion of Multigene Families in Leishmaniinae Parasites That Are Close Relatives of Leishmania spp. Pathogens 2023, 12, 1409. https://doi.org/10.3390/pathogens12121409
Tullume-Vergara PO, Caicedo KYO, Tantalean JFC, Serrano MG, Buck GA, Teixeira MMG, Shaw JJ, Alves JMP. Genomes of Endotrypanum monterogeii from Panama and Zelonia costaricensis from Brazil: Expansion of Multigene Families in Leishmaniinae Parasites That Are Close Relatives of Leishmania spp. Pathogens. 2023; 12(12):1409. https://doi.org/10.3390/pathogens12121409
Chicago/Turabian StyleTullume-Vergara, Percy O., Kelly Y. O. Caicedo, Jose F. C. Tantalean, Myrna G. Serrano, Gregory A. Buck, Marta M. G. Teixeira, Jeffrey J. Shaw, and Joao M. P. Alves. 2023. "Genomes of Endotrypanum monterogeii from Panama and Zelonia costaricensis from Brazil: Expansion of Multigene Families in Leishmaniinae Parasites That Are Close Relatives of Leishmania spp." Pathogens 12, no. 12: 1409. https://doi.org/10.3390/pathogens12121409