Comparison of Ultrastructure, Extracellular Matrix, and Drug Susceptibility in M. avium subs. hominissuis Biofilms
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Media
2.2. Biofilm Culture Conditions
2.3. Scanning Electron Microscopy (SEM)
2.4. Calcofluor White Staining
2.5. Biofilm Enzymatic Degradation Assay
2.6. Antimicrobial Susceptibility
3. Results
3.1. Biofilm Models Display Differences in Ultrastructure and Cell Morphologies
3.2. Calcofluor White Staining Suggests the Presence of Cellulose in Biofilm ECM in Both Models
3.3. Biofilm Models Differ in Structurally Important ECM Molecules
3.4. Biofilm Resident M. avium Is Resistant to Killing by Antimicrobial Drugs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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McManus, W.R.; Schorey, J.S. Comparison of Ultrastructure, Extracellular Matrix, and Drug Susceptibility in M. avium subs. hominissuis Biofilms. Pathogens 2023, 12, 1427. https://doi.org/10.3390/pathogens12121427
McManus WR, Schorey JS. Comparison of Ultrastructure, Extracellular Matrix, and Drug Susceptibility in M. avium subs. hominissuis Biofilms. Pathogens. 2023; 12(12):1427. https://doi.org/10.3390/pathogens12121427
Chicago/Turabian StyleMcManus, William R., and Jeffrey S. Schorey. 2023. "Comparison of Ultrastructure, Extracellular Matrix, and Drug Susceptibility in M. avium subs. hominissuis Biofilms" Pathogens 12, no. 12: 1427. https://doi.org/10.3390/pathogens12121427