Silencing RNA-Mediated Knockdown of IFITM3 Enhances Senecavirus A Replication
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Cultures and Plasmid Transfection
2.2. siRNA-Mediated Knockdown of IFITM3 in H1299 Cells
2.3. Quantitative Reverse Transcription PCR (qRT-PCR)
2.4. Western Blot Analysis
2.5. Confocal Immunofluorescence Analysis
2.6. Statistical Analysis
3. Results
3.1. Knockdown of Endogenous IFITM3-Enhanced SVA Replication
3.2. Overexpression of Exogenous IFITM3-Enhanced SVA Replication
3.3. Overexpression of IFITM3 in NCI-H1299 Induces Autophagy
3.4. Autophagy Inhibition Reduces SVA Replication in NCI-H1299 Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Aftab, S.; Nelson, E.; Hildreth, M.; Wang, X. Silencing RNA-Mediated Knockdown of IFITM3 Enhances Senecavirus A Replication. Pathogens 2024, 13, 290. https://doi.org/10.3390/pathogens13040290
Aftab S, Nelson E, Hildreth M, Wang X. Silencing RNA-Mediated Knockdown of IFITM3 Enhances Senecavirus A Replication. Pathogens. 2024; 13(4):290. https://doi.org/10.3390/pathogens13040290
Chicago/Turabian StyleAftab, Shamiq, Eric Nelson, Michael Hildreth, and Xiuqing Wang. 2024. "Silencing RNA-Mediated Knockdown of IFITM3 Enhances Senecavirus A Replication" Pathogens 13, no. 4: 290. https://doi.org/10.3390/pathogens13040290