Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region
Abstract
:1. Introduction
2. Results and Discussion
Sample Analyzed | Species Identified | Sequence Type | % Similarity (bp) | Reference |
---|---|---|---|---|
GYSW-1 | Rhizomucor variabilis | rRNA | 100 (291) | JF904893, This study |
GYSW-2 | Rhizomucor variabilis | rRNA | 100 (291) | JF904893, This study |
GYSW-3 | Rhizomucor variabilis | rRNA | 100 (291) | JF904893, This study |
GYSW-4 | Rhizomucor variabilis | rRNA | 100 (291) | JF904893, This study |
GYSW-5 | Rhizomucor variabilis | rRNA | 100 (291) | JF904893, This study |
GYSW-6 | Rhizomucor variabilis | rRNA | 100 (288) | JF904893, This study |
GYSW-7 | Rhizomucor variabilis | rRNA | 100 (288) | JF904893, This study |
GYSE-1 | Rhizomucor variabilis | rRNA | 100 (288) | JF904893, This study |
GYSE-2 | Rhizomucor variabilis | rRNA | 100 (288) | JF904893, This study |
GYSE-3 | Rhizomucor variabilis | rRNA | 100 (288) | JF904893, This study |
GYSE-4 | Rhizomucor variabilis | rRNA | 100 (288) | JF904893, This study |
GYSE-5 | Rhizomucor variabilis | rRNA | 100 (291) | JF904893, This study |
GYSE-6 | Rhizomucor variabilis | rRNA | 100 (291) | JF904893, This study |
GYSE-7 | Rhizomucor variabilis | rRNA | 100 (291) | JF904893,This study |
GYSE-8 | Rhizomucor variabilis | rRNA | 100 (291) | JF904893, This study |
3. Experimental Section
3.1. Isolation of Salmonella, Listeria, Staphylococcus, Bacillus and E. coli from Greek Yogurt
3.2. Isolation of Fungi from the Unopened Containers of Greek Yogurt
3.3. DNA Extraction
3.4. PCR Amplification
3.5. Nucleotide Sequencing
3.6. Data Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sulaiman, I.M.; Jacobs, E.; Simpson, S.; Kerdahi, K. Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region. Pathogens 2014, 3, 499-509. https://doi.org/10.3390/pathogens3030499
Sulaiman IM, Jacobs E, Simpson S, Kerdahi K. Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region. Pathogens. 2014; 3(3):499-509. https://doi.org/10.3390/pathogens3030499
Chicago/Turabian StyleSulaiman, Irshad M., Emily Jacobs, Steven Simpson, and Khalil Kerdahi. 2014. "Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region" Pathogens 3, no. 3: 499-509. https://doi.org/10.3390/pathogens3030499