Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection of Theileria haneyi
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and DNA Extraction
2.2. Selection for Specific Primers and Probes
2.3. Plasmid Construction and Standard Curve Generation
2.4. Real-Time Quantitative PCR
2.5. Nested PCR Amplification of the Chr1sco of T. haneyi
2.6. Sensitivity and Specificity
3. Results
3.1. Standard Curve for the Real-Time Quantitative PCR Assay
3.2. Analytical Sensitivity of the Real-Time Quantitative PCR Assay
3.3. Specificity of the Real-Time Quantitative PCR Assay
3.4. Clinical Performance
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer Name | Primer Sequences (5′-3′) | Products |
---|---|---|
T. haneyi-F: | AATCCAAAACCAGCT | 97 bp |
T. haneyi-R: | GTACAAATCTCCCTAGAG | |
T. haneyi-Probe: | FAM-CCTCCAAGTCGTCGT-MGB |
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Zhou, B.; Yang, G.; Hu, Z.; Chen, K.; Guo, W.; Wang, X.; Du, C. Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection of Theileria haneyi. Microorganisms 2023, 11, 2633. https://doi.org/10.3390/microorganisms11112633
Zhou B, Yang G, Hu Z, Chen K, Guo W, Wang X, Du C. Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection of Theileria haneyi. Microorganisms. 2023; 11(11):2633. https://doi.org/10.3390/microorganisms11112633
Chicago/Turabian StyleZhou, Bingqian, Guangpu Yang, Zhe Hu, Kewei Chen, Wei Guo, Xiaojun Wang, and Cheng Du. 2023. "Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection of Theileria haneyi" Microorganisms 11, no. 11: 2633. https://doi.org/10.3390/microorganisms11112633