Vibrio Proteases for Biomedical Applications: Modulating the Proteolytic Secretome of V. alginolyticus and V. parahaemolyticus for Improved Enzymes Production
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microbiological Methods
2.1.1. Culture Media and Conditions
2.1.2. Type Strains
2.2. SDS Electrophoresis and Zymography
2.3. Substrate Specific Activity Determination
2.4. Sequence and Structural Analyses of Collagenases
3. Results and Discussions
3.1. Culture Media and Growth Performances
3.2. Collagenase Production
3.3. Structural Analysis of Collagenases from V. parahaemolyticus and V. alginolyticus
3.4. Other Secreted Proteases
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Salamone, M.; Nicosia, A.; Ghersi, G.; Tagliavia, M. Vibrio Proteases for Biomedical Applications: Modulating the Proteolytic Secretome of V. alginolyticus and V. parahaemolyticus for Improved Enzymes Production. Microorganisms 2019, 7, 387. https://doi.org/10.3390/microorganisms7100387
Salamone M, Nicosia A, Ghersi G, Tagliavia M. Vibrio Proteases for Biomedical Applications: Modulating the Proteolytic Secretome of V. alginolyticus and V. parahaemolyticus for Improved Enzymes Production. Microorganisms. 2019; 7(10):387. https://doi.org/10.3390/microorganisms7100387
Chicago/Turabian StyleSalamone, Monica, Aldo Nicosia, Giulio Ghersi, and Marcello Tagliavia. 2019. "Vibrio Proteases for Biomedical Applications: Modulating the Proteolytic Secretome of V. alginolyticus and V. parahaemolyticus for Improved Enzymes Production" Microorganisms 7, no. 10: 387. https://doi.org/10.3390/microorganisms7100387