Rumen Development of Tianhua Mutton Sheep Was Better than That of Gansu Alpine Fine Wool Sheep under Grazing Conditions
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. H&E Stain
2.3. Determination of Rumen VFAs
2.4. DNA Extraction from Gastrointestinal Contents
2.5. 16S rRNA Full-Length Sequencing
2.6. Sequencing Data Processing
2.7. Statistical Analysis
3. Results
3.1. Differences in Volatile Fatty Acids in Rumen Contents of Tianhua Mutton Sheep and Gansu Alpine Fine Wool Sheep
3.2. Differences in Rumen Histomorphology between Tianhua Mutton Sheep and Gansu Alpine Fine Wool Sheep
3.3. Alpha Diversity Analysis of Rumen Flora in Tianhua Mutton Sheep and Gansu Alpine Fine Wool Sheep
3.4. Beta Diversity Analysis of Rumen Flora in Tianhua Mutton Sheep and Gansu Alpine Fine Wool Sheep
3.5. Analysis of the Difference in Species Composition of the Rumen Flora of Tianhua Mutton Sheep and Gansu Alpine Fine Wool Sheep
3.6. LEfSe Analysis of Rumen Flora in Tianhua Mutton Sheep and Gansu Alpine Fine Wool Sheep
3.7. Predictive Analysis of Metabolic Pathways and Functions of Rumen Flora in Tianhua Mutton Sheep and Gansu Alpine Fine Wool Sheep
3.8. Correlation Alysis of Dominant Bacteria with Histological Morphology and VFAs Indexes
4. Discussion
4.1. Differences in Volatile Fatty Acids in the Rumen Contents of Tianhua Mutton Sheep and Gansu Alpine Fine Wool Sheep
4.2. Differences in Rumen Histomorphology between Tianhua Mutton Sheep and Gansu Alpine Fine Wool Sheep
4.3. Differences in the Rumen Flora Structure of Tianhua Mutton Sheep and Gansu Alpine Fine Wool Sheep
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Items | Tianhua Mutton Sheep | Gansu Alpine Fine Wool Sheep | p-Value |
---|---|---|---|
acetic acid, mmol/L | 43.47 ± 1.51 a | 36.38 ± 1.55 b | 0.010 |
Propanoic acid, mmol/L | 15.74 ± 1.55 | 11.85 ± 2.35 | 0.123 |
Butyric acid, mmol/L | 14.88 ± 0.35 | 12.07 ± 2.22 | 0.151 |
Total acid 1, mmol/L | 84.53 ± 3.82 a | 71.56 ± 5.03 b | 0.044 |
A:P 2 | 2.74 ± 0.20 | 3.17 ± 0.51 | 0.329 |
Items | Tianhua Mutton Sheep | Gansu Alpine Fine Wool Sheep | p-Value |
---|---|---|---|
Rumen papilla height, μm | 1715.17 ± 43.28 a | 1279.80 ± 144.79 b | 0.015 |
Rumen papilla width, μm | 522.97 ± 145.39 | 462.03 ± 39.98 | 0.598 |
Stratum corneum thickness, μm | 91.53 ± 15.80 | 84.17 ± 5.25 | 0.565 |
Muscular thickness, μm | 1246.00 ± 180.92 | 1240.23 ± 11.95 | 0.966 |
Items | Tianhua Mutton Sheep | Gansu Alpine Fine Wool Sheep | p-Value |
---|---|---|---|
Feature | 4134.00 ± 234.11 a | 3094.17 ± 443.57 b | 0.001 |
ACE | 5754.15 ± 286.24 a | 4788.52 ± 431.60 b | 0.002 |
Chao1 | 5858.27 ± 318.77 a | 4578.07 ± 583.13 b | 0.002 |
Simpson | 1.00 ± 0.01 | 1.00 ± 0.01 | 0.141 |
Shannon | 10.50 ± 0.17 a | 9.94 ± 0.40 b | 0.016 |
PD_whole_tree | 230.18 ± 8.12 a | 185.54 ± 14.68 b | 0.001 |
Coverage | 0.95 ± 0.01 | 0.94 ± 0.01 | 0.279 |
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Li, D.; Liu, Z.; Duan, X.; Wang, C.; Chen, Z.; Zhang, M.; Li, X.; Ma, Y. Rumen Development of Tianhua Mutton Sheep Was Better than That of Gansu Alpine Fine Wool Sheep under Grazing Conditions. Animals 2024, 14, 1259. https://doi.org/10.3390/ani14091259
Li D, Liu Z, Duan X, Wang C, Chen Z, Zhang M, Li X, Ma Y. Rumen Development of Tianhua Mutton Sheep Was Better than That of Gansu Alpine Fine Wool Sheep under Grazing Conditions. Animals. 2024; 14(9):1259. https://doi.org/10.3390/ani14091259
Chicago/Turabian StyleLi, Dengpan, Zhanjing Liu, Xinming Duan, Chunhui Wang, Zengping Chen, Muyang Zhang, Xujie Li, and Youji Ma. 2024. "Rumen Development of Tianhua Mutton Sheep Was Better than That of Gansu Alpine Fine Wool Sheep under Grazing Conditions" Animals 14, no. 9: 1259. https://doi.org/10.3390/ani14091259