The Effect of Foam-Recycle on Ammonium Removal by Aerobic Denitrification Using Alcaligenes faecalis No. 4
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microorganism Used
2.2. Medium
2.3. Aerated Batch Cultivation
2.4. Shaking-Flask Cultivation
2.5. Activities of Hydroxylamine Oxidoreductase (HAO) and Nitrite Reductase (NIR)
2.6. Analytical Methods
3. Results and Discussion
3.1. Effect of Foam-Recycle
3.2. Consumption of Other Nitrogen Sources
3.3. Enzyme Activity
3.4. Effect of Initial Ammonium Load and Airflow Rate
3.5. Effect of pH
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Without Foam Recycling | Foam Recycling | |
---|---|---|
Initial NH4+-N (mg/L) | 1037 ± 22 | 1122 ± 5.5 |
Removed N (mg/L) | 1037 ± 22 | 1122 ± 5.5 |
Intracellular N (mg/L) | 481 ± 9.8 | 509 ± 13 |
Nitrification products (mg-N/L) | 101 ± 8.7 | 30 ± 4.3 |
Stripped N (mg/L) | 46 ± 3.6 | 41 ± 4.3 |
Denitrification ratio (%) * | 39 ± 1.1 | 48 ± 0.4 |
Maximum ammonium removal rate (mg-N/L·h) | 26 ± 0.9 | 25 ± 1.2 |
Foam | Culture of No. 4 | |
---|---|---|
Cell number (cfu/mL) | 2.1 ± 2.1 × 1010 | 4.0 ± 1.2 × 109 |
Activity (U/mL) * | 34.3 ± 3.2 | 1.25 ± 0.1 |
Specific Activity (U/cfu) | 1.5 × 10−9 | 3.3 × 10−10 |
Airflow Rate (L/min) | Initial Ammonium Level (mg-N/L) | |||
---|---|---|---|---|
3000 | 1000 | 250 | ||
Initial N (mg/L) Removed N (mg/L) (%) Intracellular N (mg/L) (%) * Nitrification N (mg/L) (%) * Stripped N (mg/L) (%) * Denitrification ratio (%) * Maximum ammonium removal rate (mg-N/L·h) | 0.2 | 2965 | 1167 | 217 |
990 (33) | 617 (53) | 217 (100) | ||
378 (38) | 318 (52) | 110 (51) | ||
19 (2) | 14 (2) | 13 (6) | ||
289 (29) | 40 (6) | 2.5 (1) | ||
31 | 40 | 42 | ||
13 | 11 | 11 | ||
Initial N (mg/L) Removed N (mg/L) (%) Intracellular N (mg/L) (%) * Nitrification N (mg/L (%) * Stripped N (mg/L) (%) * Denitrification ratio (%) * Maximum ammonium removal rate (mg-N/L·h) | 0.5 | 2975 | 1190 | 218 |
2697 (91) | 1063 (89) | 218 (100) | ||
546 (20) | 397 (37) | 124 (57) | ||
39 (2) | 29 (3) | 21 (10) | ||
333 (12) | 59 (6) | 5 (2) | ||
66 | 54 | 31 | ||
28 | 19 | 13 | ||
Initial N (mg/L) Removed N (mg/L) (%) Intracellular N (mg/L) (%) * Nitrification N (mg/L) (%) * Stripped N (mg/L) (%) * Denitrification ratio (%) * Maximum ammonium removal rate (mg-N/L·h) | 0.8 | 2967 | 1177 | 225 |
2967 (100) | 1104 (94) | 225 (100) | ||
668 (23) | 404 (37) | 128 (57) | ||
56 (2) | 38 (3) | 24 (11) | ||
354 (12) | 72 (6) | 4 (2) | ||
64 | 54 | 31 | ||
39 | 21 | 15 |
pH 6 | pH 7 | pH 8 | |
---|---|---|---|
Initial NH4+-N (mg/L) | 1186 | 1122 | 1169 |
Removed N (mg/L) | 1165 | 1122 | 1159 |
Intracellular N (mg/L) | 424 | 509 | 521 |
Nitrification N (mg/L) | 25 | 30 | 21 |
Stripped N (mg/L) | 79 | 41 | 170 |
Denitrification ratio (%) * | 55 | 48 | 39 |
Maximum ammonium removal rate (mg-N/L) | 20 | 25 | 15 |
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Lee, K.; Joo, H.-S. The Effect of Foam-Recycle on Ammonium Removal by Aerobic Denitrification Using Alcaligenes faecalis No. 4. Environments 2023, 10, 184. https://doi.org/10.3390/environments10100184
Lee K, Joo H-S. The Effect of Foam-Recycle on Ammonium Removal by Aerobic Denitrification Using Alcaligenes faecalis No. 4. Environments. 2023; 10(10):184. https://doi.org/10.3390/environments10100184
Chicago/Turabian StyleLee, Kwanyong, and Hung-Soo Joo. 2023. "The Effect of Foam-Recycle on Ammonium Removal by Aerobic Denitrification Using Alcaligenes faecalis No. 4" Environments 10, no. 10: 184. https://doi.org/10.3390/environments10100184