Can Archival Tissue Reveal Answers to Modern Research Questions?: Computer-Aided Histological Assessment of Neuroblastoma Tumours Collected over 60 Years
Abstract
:1. Introduction
2. Experimental Section
2.1. Neuroblastoma Cases and TMA Construction
2.2. Immunohistochemistry
2.3. Evaluation of TMA Immunohistochemistry
2.3.1. Digital Image Acquisition
2.3.2. Computer-Aided Image Analysis: Cell Counting
2.3.3. Computer-Aided Image Analysis: Pixel Counting for Signal Intensity
2.4. Statistical Analysis
3. Results and Discussion
3.1. Neuroblastoma TMA Core Morphology
3.2. Immunohistochemistry
3.3. Computer-Aided Image Analysis
3.3.1. Neuroblastoma Marker NB84
Marker | Linear Regression (R) | p value | |
---|---|---|---|
Diagnostic | NB84 | 0.432 | <0.001 |
Chromogranin A | −0.138 | 0.144 | |
NSE | 0.209 | 0.026 | |
Ki-67 | 0.177 | 0.066 | |
INI7 | 0.072 | 0.452 | |
Neurofilament Protein | −0.050 | 0.607 | |
S100 | −0.258 | 0.006 | |
CD99 | −0.200 | 0.039 | |
Synaptophysin | −0.109 | 0.265 | |
Research | IL-7 | −0.033 | 0.724 |
IL-7R | 0.037 | 0.698 | |
JAK1 | 0.006 | 0.951 | |
JAK3 | 0.065 | 0.501 | |
STAT5 | −0.018 | 0.855 |
3.3.2. Neuron Specific Enolase
3.3.3. S100A
3.3.4. CD99
3.3.5. Research Antibodies: Interleukin-7, Interleukin-7 Receptor, Stat5, JAK1, and JAK3
3.3.6. Chromogranin A, Ki-67, INI1, Neurofilament Protein and Synaptophysin.
4. Conclusions
Acknowledgments
Author Contributions
Conflict of Interest
References
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Chetcuti, A.; Mackie, N.; Tafavogh, S.; Graf, N.; Henwood, T.; Charlton, A.; Catchpoole, D. Can Archival Tissue Reveal Answers to Modern Research Questions?: Computer-Aided Histological Assessment of Neuroblastoma Tumours Collected over 60 Years. Microarrays 2014, 3, 72-88. https://doi.org/10.3390/microarrays3010072
Chetcuti A, Mackie N, Tafavogh S, Graf N, Henwood T, Charlton A, Catchpoole D. Can Archival Tissue Reveal Answers to Modern Research Questions?: Computer-Aided Histological Assessment of Neuroblastoma Tumours Collected over 60 Years. Microarrays. 2014; 3(1):72-88. https://doi.org/10.3390/microarrays3010072
Chicago/Turabian StyleChetcuti, Albert, Nicole Mackie, Siamak Tafavogh, Nicole Graf, Tony Henwood, Amanda Charlton, and Daniel Catchpoole. 2014. "Can Archival Tissue Reveal Answers to Modern Research Questions?: Computer-Aided Histological Assessment of Neuroblastoma Tumours Collected over 60 Years" Microarrays 3, no. 1: 72-88. https://doi.org/10.3390/microarrays3010072