Sex-dependent Differences in the Bioenergetics of Liver and Muscle Mitochondria from Mice Containing a Deletion for glutaredoxin-2
Abstract
:1. Introduction
2. Experimental
2.1. Chemicals
2.2. Animals and Genotyping
2.3. Mitochondrial Preparation
2.4. Quantification of Hydrogen Peroxide Production
2.5. Measurement of Mitochondrial Respiration:
2.6. Data Analysis
3. Results
3.1. Loss of GRX2 does not Alter H2O2 Production in Liver Mitochondria
3.2. GRX2 Deficiency Alters ROS Release from Liver Mitochondria Prepared from Male Mice Only
3.3. Impact of Deleting the Grx2 Gene on Liver Mitochondria Bioenergetics
3.4. Effect of GRX2 Deficiency on the Bioenergetics of Skeletal Muscle Mitochondria
3.5. Sex Differences in ROS Production by Muscle Mitochondria Isolated from GRX2-Deficient Mice
3.6. Sex Differences in the Bioenergetics of Muscle Mitochondria from GRX2-Deficient Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Mallay, S.; Gill, R.; Young, A.; Mailloux, R.J. Sex-dependent Differences in the Bioenergetics of Liver and Muscle Mitochondria from Mice Containing a Deletion for glutaredoxin-2. Antioxidants 2019, 8, 245. https://doi.org/10.3390/antiox8080245
Mallay S, Gill R, Young A, Mailloux RJ. Sex-dependent Differences in the Bioenergetics of Liver and Muscle Mitochondria from Mice Containing a Deletion for glutaredoxin-2. Antioxidants. 2019; 8(8):245. https://doi.org/10.3390/antiox8080245
Chicago/Turabian StyleMallay, Sarah, Robert Gill, Adrian Young, and Ryan J. Mailloux. 2019. "Sex-dependent Differences in the Bioenergetics of Liver and Muscle Mitochondria from Mice Containing a Deletion for glutaredoxin-2" Antioxidants 8, no. 8: 245. https://doi.org/10.3390/antiox8080245
APA StyleMallay, S., Gill, R., Young, A., & Mailloux, R. J. (2019). Sex-dependent Differences in the Bioenergetics of Liver and Muscle Mitochondria from Mice Containing a Deletion for glutaredoxin-2. Antioxidants, 8(8), 245. https://doi.org/10.3390/antiox8080245