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Nanomaterials 2013, 3(2), 221-228; doi:10.3390/nano3020221

Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada
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Received: 18 March 2013 / Revised: 27 March 2013 / Accepted: 28 March 2013 / Published: 4 April 2013
(This article belongs to the Special Issue Nanomaterials in Sensors)
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Abstract

Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated.
Keywords: graphene; DNA; adsorption; fluorescence; separation; gel electrophoresis graphene; DNA; adsorption; fluorescence; separation; gel electrophoresis
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Huang, P.-J.J.; Liu, J. Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide. Nanomaterials 2013, 3, 221-228.

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