Size-Based Sorting and In Situ Clonal Expansion of Single Cells Using Microfluidics
Abstract
:1. Introduction
2. Materials and Methods
2.1. Device Design and Fabrication
2.2. Numerical Studies of the Flow Field
2.3. Numerical Studies of the Cell Deformation
2.4. Isolation of K562 Cells in Culture Media
2.5. Isolation of k562 Cells in Whole Blood
2.6. In Situ Clonal Expansion of Trapped Single Cells
2.7. Cell Lineage Tracking and Data Analysis
3. Results
3.1. Numerical Studies of the Flow Field
3.2. Numerical Studies of the Cell Deformation
3.3. Isolation of K562 Cells from Culture Media
3.4. Screening of K562 Cells from Whole Blood
3.5. In Situ Clonal Expansion of Trapped Single Cells
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chen, H.; Meng, H.; Chen, Z.; Wang, T.; Chen, C.; Zhu, Y.; Jin, J. Size-Based Sorting and In Situ Clonal Expansion of Single Cells Using Microfluidics. Biosensors 2022, 12, 1100. https://doi.org/10.3390/bios12121100
Chen H, Meng H, Chen Z, Wang T, Chen C, Zhu Y, Jin J. Size-Based Sorting and In Situ Clonal Expansion of Single Cells Using Microfluidics. Biosensors. 2022; 12(12):1100. https://doi.org/10.3390/bios12121100
Chicago/Turabian StyleChen, Huaying, Haixu Meng, Zhenlin Chen, Tong Wang, Chuanpin Chen, Yonggang Zhu, and Jing Jin. 2022. "Size-Based Sorting and In Situ Clonal Expansion of Single Cells Using Microfluidics" Biosensors 12, no. 12: 1100. https://doi.org/10.3390/bios12121100