HBD3 Induces PD-L1 Expression on Head and Neck Squamous Cell Carcinoma Cell Lines
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. PD-L1 Inducers
4.2. Cell Lines
4.3. Immunohistochemistry
4.4. Induction of PD-L1 Expression
4.5. Detection of Cells Expressing PD-L1 by Flow Cytometry
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cell Line | Sex | Anatomical Site (Oral Cavity) | TNM Stage |
---|---|---|---|
SCC4 | M | Tongue | T3N0M0 |
SCC15 | M | Tongue | T4N1M0 |
SCC25 | M | Tongue | T2N1 |
UM-SCC19 | M | Oropharynx/base of tongue | T2N1M0 |
UM-SCC99 | M | Oropharynx | T3N0M0 |
Cell Line | N | Mean | SD | Minimum | Maximum | Median |
---|---|---|---|---|---|---|
SCC4 | 3 | 241.40 | 58.79 | 207.17 | 309.28 | 207.75 |
SCC15 | 3 | 193.54 | 93.45 | 125.91 | 300.17 | 154.53 |
SCC19 | 3 | 92.65 | 15.09 | 79.67 | 109.21 | 89.08 |
SCC25 | 3 | 387.59 | 135.59 | 279.68 | 539.79 | 343.29 |
SCC99 | 3 | 206.16 | 47.72 | 173.10 | 260.87 | 184.52 |
Squamous Cell Carcinoma (SCC) Cell Lines | Unstained Cell Controls (%) | Isotype Ab Treated Cell Controls (%) | No-Treatment Control Cells (%) | 0.6 µM IFNγ Treated Cells (%) | 20.0 µM HBD3 Treated Cells (%) |
---|---|---|---|---|---|
SCC4 | 0.11 a | 0.30 | 1.79 | 5.90 | 2.64 |
(0.0) b | (229.6) | (47.5) | |||
SCC15 | 0.05 | 0.04 | 5.34 | 40.45 | 11.16 |
(0.0) | (657.5) | (109.0) | |||
SCC19 | 0.10 | 0.09 | 89.40 | 94.60 | 96.50 |
(0.0) | (5.8) | (7.9) | |||
SCC25 | 0.04 | 0.16 | 12.28 | 13.68 | 16.75 |
(0.0) | (11.4) | (36.4) | |||
SCC99 | 0.02 | 0.29 | 40.30 | 63.60 | 70.40 |
(0.0) | (57.8) | (74.7) |
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Gomez Hernandez, M.P.; Bates, A.M.; Starman, E.E.; Lanzel, E.A.; Comnick, C.; Xie, X.J.; Brogden, K.A. HBD3 Induces PD-L1 Expression on Head and Neck Squamous Cell Carcinoma Cell Lines. Antibiotics 2019, 8, 161. https://doi.org/10.3390/antibiotics8040161
Gomez Hernandez MP, Bates AM, Starman EE, Lanzel EA, Comnick C, Xie XJ, Brogden KA. HBD3 Induces PD-L1 Expression on Head and Neck Squamous Cell Carcinoma Cell Lines. Antibiotics. 2019; 8(4):161. https://doi.org/10.3390/antibiotics8040161
Chicago/Turabian StyleGomez Hernandez, Maria Paula, Amber M. Bates, Emily E. Starman, Emily A. Lanzel, Carissa Comnick, Xian Jin Xie, and Kim A. Brogden. 2019. "HBD3 Induces PD-L1 Expression on Head and Neck Squamous Cell Carcinoma Cell Lines" Antibiotics 8, no. 4: 161. https://doi.org/10.3390/antibiotics8040161