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Article

Preservative Effects of Gelatin Active Coating Enriched with Eugenol Emulsion on Chinese Seabass (Lateolabrax maculatus) during Superchilling (−0.9 °C) Storage

1
Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai 201306, China
2
Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai 201306, China
3
National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai 201306, China
4
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
*
Authors to whom correspondence should be addressed.
Coatings 2019, 9(8), 489; https://doi.org/10.3390/coatings9080489
Submission received: 13 July 2019 / Revised: 29 July 2019 / Accepted: 31 July 2019 / Published: 1 August 2019
(This article belongs to the Special Issue Edible Films and Coatings: Fundamentals and Applications)

Abstract

:
This research was to evaluate the effects of gelatin (G) active coating containing eugenol/β-cyclodextrin (βCD) emulsions combined with superchilling (−0.9 °C) on physicochemical, microbiological, and organoleptic properties of Chinese seabass samples during 30 days of storage. Results showed that seabass samples dipped in G-βCD coatings containing 0.15% or 0.3% eugenol combined with superchilling could significantly lower the total volatile basic nitrogen, K value, total viable count, H2S-producing bacteria, Pseudomonas spp. and Psychrophilic counts, and free fatty acids. Further, G-βCD coatings containing eugenol with superchilling (−0.9 °C) were more effective in retarding the water migration by low field NMR and MRI results, maintaining quality of seabass during storage according to organoleptic evaluation results.

1. Introduction

Chinese seabass (Lateolabrax maculatus) is recognized as one of the most important mariculture fish in China, which has an annual production exceeding 12 million tons [1,2]. Its high protein and low fat content make it a value-added seafood product with increasing demand [3]. However, gutted seabass is extremely susceptible to endogenous enzymes and exogenous spoilage microflora, resulting in lipid oxidation, protein degradation, or decomposition [4]. A low temperature is very important to retard bacterial growth and reduce enzyme activity to extend the shelf life of fish for some days [5]. Superchilling is the process of lowering the temperature of a product just below its initial freezing temperature and the proportion of water frozen is preserved approximately 5–30% within the food product [6,7,8,9]. Superchilling has been used in the fish processing to significantly increase the shelf life and has been successfully applied in preservation of Atlantic mackerel [10], hairtail [11], olive flounder [12], seabream [13], as well as other seafood products.
Besides the temperature control, active coating is considered to be an effective and environmentally friendly method of keeping aquatic products fresh [14,15]. Gelatin is widely used in preparing the active coating, but the pure gelatin coating has poor antioxidant and antimicrobial abilities and does not extend the shelf life of seafood [16]. In recent years, some natural antioxidant or antibacterial compounds have been researched for their preservative activities for seafood. Eugenol (E) is a natural phenolic compound found as a major compound in clove essential oil with antioxidant and antimicrobial properties [17]. However, eugenol is associated with strong flavor altering the original food flavor and high hydrophobicity posing a great challenge to its direct incorporation into foods [18]. To minimize its adverse effects, eugenol has been encapsulated in systems suitable for food application [19,20].
Therefore, the objective of our research was to evaluate the preservative effects of gelatin active coating containing eugenol/β-cyclodextrin (βCD) emulsions on Chinese seabass during superchilling storage at −0.9 °C, in the aspects of pH, water distribution and migration, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA) value, microbiological analysis, K-values, free amino acids (FAAs) analysis, and sensorial characteristics.

2. Material and Methods

2.1. Preparation of Gelatin Active Coating Containing Eugenol/βCD Emulsions

The eugenol/βCD emulsions were prepared in the same method as described by Sun et al. [21] and Shao et al. [22] with some modifications. Eugenol (0.075%, 0.15% and 0.3%) and 750 mg βCD were stirred mechanically in a beaker, respectively, and 5 g Tween 80 was added to make them homogeneously dispersed. Then 40 mL ultrapure water was added and stirred for 8 h at room temperature and more ultrapure water was added to a final emulsion volume of 100 mL. The emulsion was obtained by continuous stirring for another 6 h. The corresponding concentrations of eugenol in the eugenol/βCD emulsions were 0.075%, 0.15%, 0.3%. Gelatin (G, 6% w/w, Bloom value at 240–270, BBI Life Science, Shanghai, China) and glycerol (1.5% v/w) were dissolved in prepared eugenol/βCD emulsions (100 mL) at 45 °C and stirred for 2 h. Then, the mixture was treated with an ultrasonic homogenizer (XEB-1000-P, Xiecheng Ultrasonic Equipment co. LTD, Dongguan, China) at 20 KHz with the powder of 800 W for 10 min to obtain homogeneous coating solutions. The final active coating solutions were marked as G-βCD, G-βCD-0.075%E, G-βCD-0.15%E, and G-βCD-0.3%E, respectively.

2.2. Preparation of Seabass and Immersion Sample Treatment

A total of 72 live Chinese seabass (Lateolabrax maculatus) with an average weight of 800 ± 10 g were supplied by a local market in Luchao Port town (Shanghai, China). They were stunned with ice for 15 min then killed and the gill and viscera of seabass were removed. Then they were thoroughly washed with sterilized 1% NaCl solutions and 2 random seabass samples were taken to determine the basic quality profiles at initial sampling point (day 0). The remaining seabass samples were divided into 5 batches (14 fish per batch) for (1) CK; (2) G-βCD; (3) G-βCD-0.075%E; (4) G-βCD-0.15%E; and (5) G-βCD-0.3%E. Different batches of seabass samples were immersed in the corresponding freshly prepared coating solutions for 10 min at 4 °C with a solution ratio of 1:3 (w/v), then the seabass samples were taken out and put into a sterile biochemical incubator with air flow at 4 °C for 60 min to form the coating. After that, each seabass sample was individually packed in a sterile polyethylene bag and stored at −0.9 ± 0.1 °C in a refrigerator (BPS-250CB, Yiheng Thermostatic Chamber, Shanghai, China). Fish samples were selected randomly for analysis at 0, 5, 10, 18, 18, 21, 24, and 27 days.

2.3. pH Measurement

The pH measurement determination were determined according to Kim et al. [23]. A precise amount of minced seabass muscle (5 g) homogenized with 45 mL distilled water, then filtered after 30 min. The filtrate pH was measured by pH meter.

2.4. Water Distribution and Migration

The proton relaxation experiments were performed proposed by Li et al. [24]. The fish samples from the seabass muscle were cut into small squares (2.5 × 2 × 1.3 cm3, about 5 g) and sealed with polyethylene films. Transverse relaxation T2 measurements were executed on a LF-NMR analyzer (Niumag MesoMR23-060H.I, Suzhou, China) with a proton resonance frequency of 20 MHz. For each measurement 16 scans were performed with 3000 echoes, the relative content of three water components was obtained from the iterative inversion with analytical software of T2 transverse relaxation time. Acquisition parameters were showed as followed: slice width = 3 mm, TR (time repetition) = 2000 and TE (time echo) = 15 ms. MRI experiments were performed to get proton density weighted images and the echo time, repetition time and slice width were 18.2 ms, 850 ms, and 2 mm, respectively.

2.5. Total Volatile Basic Nitrogen (TVB-N)

For TVB-N determinations, the distillation method of a deproteinized sample as recommended by Neira et al. [25] was used. A precise amount of minced seabass muscle (5 g) was placed into a distilling flask and blended with 1.5 g of MgO. Then steam distillation was performed with Kjeldahl nitrogen-determination apparatus (Kjeltec8400, Foss, Hillerød, Denmark) and TVB-N was expressed as mg N/100 g of seabass sample.

2.6. Evaluation of TBA Value

Lipid oxidation in seabass samples was monitored by the evaluation of thiobarbituric acid reactive substances (TBA-RS) by the method portrayed by Cheng et al. [26] with some modifications. Then, 5 g of seabass muscle was homogenized in 25 mL of 20% TBA solution, centrifuged at 8000 g for 10 min at 4 °C after stand still for 1 h and the supernatants filtered with Whatman No. 3 qualitative filter paper. The filtrate was diluted with ultrapure water to 50 mL. After that, 10 mL diluent and 10 mL TBA solution was mixed and heated at 100 °C for 15 min and then cooled to 30 °C. The absorbance of the supernatant was measured at 532 nm using a spectrophotometer (Evolution 220, Thermo Fisher Scientific, Waltham, MA, USA) and the TBA-RS values were expressed as mg of malonaldehyde (MDA)/100 g of seabass sample.

2.7. K-Values

The ATP-related compounds were determined by a RP-HPLC procedure (Waters 2695, Milford, MA, USA) proposed by Li et al. [27] The minced seabass muscle (5 g) homogenized with 10 mL 10% perchloric acid (PCA) and centrifuged at 8000 g for 15 min at 4 °C. The precipitate was stirred with 10 mL 5% PCA and centrifuged at 8000 g for 10 min at 4 °C for 2 times. The supernatant pH was adjusted to 6.5 after the supernatant was merged and added with 15 mL distilled water. After 30 min, take the supernatant fixed in 50 mL volumetric bottle with ultrapure water. Finally, the supernatant was filtered with 0.22 μm membrane and applied to RP-HPLC procedure. The K-value was calculated as the ratio of the percentage amounts of inosine (HxR) and hypoxanthine (Hx) to the sum of adenosine-5′-triphosphate (ATP), adenosine-5′-diphosphate (ADP), adenosine-5′-monophosphate (AMP), inosine-5′-monophosphate (IMP), HxR and Hx was calculated as follows:
K   value   ( % ) = HxR + Hx ATP + ADP + AMP + IMP + HxR + Hx   × 100  

2.8. Free Amino Acid (FAA) Analysis

FAAs were determined according to Abdelhedi et al. [28] The minced samples (2.0 g) were homogenized with 10 mL of 5% trichloroacetic acid and centrifuged at 8000 g for 15 min at 4 °C. The supernatant was centrifuged again under the same conditions, then the combined supernatant was diluted to 25 mL. The 1 mL extract was filtered with a 0.22 μm disposable filter for reserve, and automatic amino acid analysis was used. The diluted solution was filtered using a 0.22 μm filter membrane and applied to Hitachi L-8800 amino acid analyzer (Tokyo, Japan).

2.9. Microbiological Analysis

Representative 5 g portions of seabass samples were blended with 45 mL of sterilized normal saline (0.85% NaCl) fully homogenized and then subjected to serial dilutions. The following microbiological analyses were performed: (i) determination of the mesophile bacteria on plate count agar medium (Hopebio, Qingdao, China) with 0.4 mg/mL nystatin (Aladdin, Shanghai, China) were incubated at 30 °C for 48 h; (ii) determination of H2S-producing bacteria on iron agar medium (Hopebio, Qingdao, China) were incubated at 30 °C for 72 h; (iii) determination of Pseudomonas spp. on cetrimide agar medium (Hopebio, Qingdao, China) were incubated at 30 °C for 72 h; and (iv) determination of psychrophilic bacteria on plate count agar medium were incubated at 4 °C for 7 days.

2.10. Organoleptic Properties

The organoleptic properties of seabass samples were assessed with the method described by Cai et al. [4]. Nine experienced judges received some training about the superchilling storage seabass samples, focusing on odor, color, mucus, tissue morphology, and elasticity using ten-grade marking system: 10.0–9.0 (excellent), 8.9–7.0 (good), 6.9–5.0 (fair), and 4.9–1.0 (rejectable).

2.11. Statistical Analysis

The one-way ANOVA procedure followed by Duncan test was used for multiple comparisons by the SPSS 22.0, and the results were expressed as means ±SD.

3. Results and Discussions

3.1. Microbiological Results

Figure 1 shows the counts corresponding to the growth of TVC, H2S-producing bacteria, Pseudomonas spp., and psychrophile of seabass samples during superchilling storage. The initial mesophiles population was 2.30 log CFU/g (Figure 1a), which was at a low microbial level for the starting seabass samples. The mesophile number increased during storage for all seabass samples and the G-βCD-3.00E group had significantly lower mesophile number than other samples during whole storage period. At day 21, CK and G-βCD should be removed due to exceeding the allowed maximum limit of 7.0 log CFU/g [29].
Eugenol could prolong the shelf life of seabass samples because of its remarkable inhibitory effect. Dimitrijević et al. [30] also demonstrated that eugenol showed good antimicrobial activity on retarding TVC growth, similar results also shown towards the increase of H2S-producing bacteria, Pseudomonas spp., and psychrophile in the current research. H2S-producing bacteria is one of the specific spoilage organisms in seabass samples during cold storage [31]. An initial count of H2S-producing bacteria in seabass samples was approximately 2.3 log CFU/g (Figure 1b). At the end of storage, samples treated with eugenol presented lower counts. Pseudomonas spp. had a similar growth pattern with that of mesophilic microbes (Figure 1c), which suggests that the aerobic spoilage bacteria dominated for seabass samples during supperchilling storage. Psychrotrophic bacteria could lead to seabass samples’ quality deterioration in odor, texture, and flavor through the production of metabolic compounds including ketones, aldehydes, volatile sulfides, and biogenic amines [32]. At the end of storage, the total psychrophilic bacteria counts for CK, G-βCD, G-βCD-0.075%E, G-βCD-0.15%E, and G-βCD-0.3%E were 8.01, 7.66, 6.95, 6.77, and 6.51 log CFU/g (Figure 1d), respectively, indicating that the seabass samples quality was retained by eugenol addition.

3.2. Chemical Results in Seabass Samples

A tendency for initially decreasing and then increasing pH values could be observed in all samples (Figure 2a). The pH reduction at the beginning was caused by the accumulation of lactic acid during glycolysis reactions and the increased pH values due to the production of volatile basic components as a result of bacterial propagation [33]. CK showed significantly (p < 0.05) higher average pH values when compared with the seabass samples dipped in G-βCD coatings containing eugenol during storage. Furthermore, smaller pH changes was observed in G-βCD-0.3%E samples and it could be concluded that G-βCD-0.3%E combined with superchilling could be have a positive synergistic effects on the inhibition of bacterial spoilage.
The TVB-N value of fresh seabass sample was 8.65 mg N/100 g fish muscle (Figure 2b) indicating satisfactory freshness of seabass samples at the beginning. TVB-N values slowly increased at the beginning and had a sharp increase from day 21. The increase TVB-N formation was especially obvious in CK and G-βCD seabass samples, however, seabass samples with G-βCD coatings containing eugenol significantly reduced the formation of TVB-N during storage comparing with the CK and G-βCD samples. The TVB-N values of CK, G-βCD, G-βCD-0.075%E, G-βCD-0.15%E, and G-βCD-0.3%E seabass samples reached to 55.41, 47.29, 35.49, 30.69, and 27.66 mg N/100 g fish muscle, respectively, at the end of storage, which signified only G-βCD-0.3%E samples did not exceed the upper limit (30 mg N/100 g) throughout superchilling storage [34]. High TVB-N values in aquatic products preservation indicates that nitrogen-containing substances accumulated from the nitrogen-containing molecules, such as protein and nucleic acid, degraded by proteolytic bacteria [35].
Changes in IMP, Hx, and K value of seabass samples during superchilling storage are presented in Figure 2c–e. IMP offers sweet and meaty flavor enhancing fish quality, however, it could be conversed to Hx representing for unpleasant bitterness in aquatic products [36]. The concentration of IMP at day 0 was 34.75 mg/100 g and decreased in all samples during superchilling (Figure 2c). At the end of storage, the concentrations of IMP in the CK, G-βCD, G-βCD-0.075%E, G-βCD-0.15%E, and G-βCD-0.3%E seabass samples were 0.17, 1.25, 2, 2.94, and 4.27 mg/100 g, respectively. The IMP concentrations in seabass samples dipped in G-βCD coatings containing eugenol were significantly (p < 0.05) higher than that of CK and G-βCD samples during superchilling storage, which demonstrated that G-βCD coatings containing eugenol could inhibit bacteria IMP degradation resulting from spoilage organism and microbial enzymes. The initial Hx concentration was 1.36 mg/100 g and increased irregularly in all samples during superchilling (Figure 2d). The CK and G-βCD seabass samples showed significantly higher (p < 0.05) Hx concentration than that of seabass samples dipped in G-βCD coatings containing eugenol, which demonstrated that eugenol addition could be efffective in controlling the microbial growth and inhibiting the formation of Hx [37].
The K value of fresh seabass samples was 14.39% and increased during superchilling storage (Figure 2e). CK and G-βCD seabass samples exceeded the maximum permissible level on day 15. The unacceptable limit for K value is 60% [38] and the CK and G-βCD seabass samples exceeded the maximum permissible level on day 15, however, the seabass samples dipped in G-βCD coatings containing eugenol remained below the rejection limit on day 21. These results indicated that G-βCD coatings containing eugenol combined with superchilling could suppress the degradation of ATP and keep good quality of seabass samples during superchilling storage.
TBARS could indicate the peroxidation of polyunsaturated fatty acids in fish and be measured as MDA equivalent [39]. The TBARS value in fresh seabass sample was 0.038 mg MDA/100 g and then increased from day 0 to day 15. Afterwards, the TBARS values decreased slightly due to MDA reacting with aldehydes and ketones [40]. TBARS occurred in rather different rates with increasing storage time, as expected, the CK seabass samples had the highest TBARS values, and TBARS could be significantly affected (p < 0.05) by eugenol concentration in the active coating solutions. The TBARS values for G-βCD-0.075%E, G-βCD-0.15%E, and G-βCD-0.3%E were 0.091, 0.089, and 0.092 mg MDA/100 g, respectively, which were lower than that of CK and G-βCD seabass samples and there were no significant differences among the treated samples. Therefore, eugenol as the antioxidant in the active coatings and coatings barrier against oxygen penetration resulting in delayed lipid oxidation [41,42,43].
The concentrations of FAAs in seabass samples on the 1st, 15th, and 30th day of superchilling storage are shown in Table 1. From Table 1, the alanine and aspartic acid concentrations in all samples sharply increased and then decreased during superchilling storage. The major amino acids in seabass samples were glycine, alanine, and histidine, which accounted for 42.86%–65.26% of total FAAs contents. As an off-taste amino acid, histidine accounted for 8.70%–12.66% of total FAA contents among all seabass samples. The content of histidine increased from 17.81 mg/100 g to 45.64 mg/100 g on day 30 in the CK samples, however, the corresponding contents in G-βCD-0.075%E, G-βCD-0.15%E, and G-βCD-0.3%E were only 36.64, 33.88, and 33.47 mg/100 g on day 30, respectively, which was caused by the oxidation process from trimethylamine oxide and led to a reduction in the bitterness for seabass samples during superchilling storage [44]. Glycine, alanine, glutamic acid, and aspartic acid are responsible for the characteristic flavor of fish [45]. Glycine contents in CK samples increased from 55.63 mg/100 g to 75.80 mg/100 g on day 15 and then decreased to 68.56 mg/100 g on day 30. Glycine contents in G-βCD-0.075%E, G-βCD-0.15%E, and G-βCD-0.3%E had similar trends comparing with CK samples, however, their final contents were significantly (p < 0.05) higher than that of CK and G-βCD. In addition, seabass samples dipped in G-βCD coatings containing eugenol significantly increased alanine content from day 0 to day 15 and the CK, G-βCD, and G-βCD-0.15%E decreased from day 15 to day 30, however, the alanine content in G-βCD-0.15%E and G-βCD-0.3%E samples increased at that time. In general, seabass samples with G-βCD coatings containing eugenol could accumulate the partial umami-associated FAAs and total FAAs and reduce off-tasting histidine, which improved flavor quality of seabass samples during superchilling storage. Active coating effectively controlled water loss and microbial metabolism to keep excellent quality parameters of seabass samples.

3.3. Water Distribution by LF NMR Analysis

T21 representing for bound water varied, ranging from 0.15% to 3.30% during superchilling storage (Figure 3a), indicating the bound water in seabass samples could be free from the influence of treated methods and storage time [46]. T22 known as immobile water within the myofibril and decreased gradually during superchilling storage (p < 0.05). T23 known as free water increased constantly. In the current research, the CK seabass samples had lower immobilized water than that of other seabass samples. Some researchers also demonstrated that water located within myofibrillar macromolecules could release or translate to free water on account of destruction of muscle fiber during storage [25,47,48,49]. At the same time, G-βCD coatings containing eugenol retarded the change rates of T22 and T23 to hinder water migration, especially for G-βCD-0.15%E and G-βCD-0.3%E samples.
MRI provides visual information for seabass samples during superchilling storage. Red color stands for high proton density and blue color stands for low proton density in the pesudo-color images. As shown in Figure 3b, the brightness of images varied obscure in the early storage and the brightness of samples were darker and bluer with the time increasing. On day 30, the color of CK seabass samples was bluer and darker than others, demonstrating that the degradation of myofibill and destruction of microstructure in CK seabass samples [50]. The brightness of seabass samples dipped in G-βCD coatings containing eugenol is lighter compared to CK and G-βCD samples on day 30, which indicated that the G-βCD coatings containing eugenol are more suitable for quality maintenance of seabass samples. The result was consistent with the variation of LF-NMR transverse relaxation.

3.4. Organoleptic Properties

The acceptability of seabass samples during superchilling storage depends upon their changes in organoleptic characteristics. Figure 4a–e display the organoleptic evaluation results for the t seabass samples including smell, color, mucus, muscular tissue and elasticity during supperchilling storage. At the beginning, all seabass samples had high organoleptic scores and they were of excellent quality, and then a significant quality loss in all seabass samples was observed (p < 0.05) during superchilling storage. However, seabass samples dipped in G-βCD coatings containing eugenol had significant higher organoleptic scores than those of the CK and G-βCD samples (p < 0.05). The CK and G-βCD seabass samples were considered unacceptable by the panelists on day 21 and day 24, respectively, when the samples were spoiled with off-odor and loose elasticity. No significant difference (p > 0.05) was detected among G-βCD-0.075%E, G-βCD-0.15%E, and G-βCD-0.3%E, and G-βCD-0.075%E on day 24, G-βCD-0.15%E, and G-βCD-0.3%E both on day 24 were not suitable for consumption suggested by panelists. The best organoleptic evaluations were reported in G-βCD-0.15%E and G-βCD-0.3%E, which were also supported by microbial and chemical quality analyses.

4. Conclusions

Seabass samples dipped in G-βCD coatings containing 0.075%, 0.15%, and 0.3% eugenol combined with superchilling (−0.9 °C) could slow down the rate of seabass samples spoilage during storage. The results of physicochemical, microbiological and organoleptic properties indicated that the G-βCD-0.15%E and G-βCD-0.3%E seabass samples maintained better physico-chemical properties organoleptic evaluation results during superchilling storage, which mainly due to that eugenol could effectively suppress the growth of spoilage microorganisms. G-βCD-0.15%E and G-βCD-0.3%E had similar effects in slowing down seabass samples spoilage, however, 0.3% eugenol addition gave the active coating solution a strong flavor. Therefore, 0.15% eugenol addition combined with superchilling (−0.9 °C) could be suitable for maintaining the freshness of seabass samples and extended the shelf life.

Author Contributions

Data Curation, Q.Z. and P.L.; Formal Analysis, Q.Z. and W.L.; Funding Acquisition, J.X.; Investigation, Q.Z., P.L. and S.F.; Methodology, Q.Z. and W.L.; Software, P.L.; Supervision, J.M. and J.X.; Validation, S.F.; Writing-Original Draft, Q.Z. and J.M.; Writing-Review & Editing, J.M. and J.X.

Funding

This research was funded by NSFC (Nos. 31571914, 31601414), China Agriculture Research System (CARS-47), National Key Research and Development Program (2016YFD0400106), Doctoral Start-Up Fund from SHOU (A2-2006-00-200310), and also supported by Shanghai Agriculture Applied Technology Development Program, China (2019-02-08-00-10-F01143), Shanghai Municipal Science and Technology Project to enhance the capabilities of the platform (No. 19DZ2284000).

Acknowledgments

The authors would like to express their profound gratitude to Weiqiang Qiu from Instrumental Analysis Center of Shanghai Ocean University for his technical assistance.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Chen, B.; Li, Y.; Peng, W.; Zhou, Z.; Shi, Y.; Pu, F.; Luo, X.; Chen, L.; Xu, P. Chromosome-level assembly of the Chinese seabass (Lateolabrax maculatus) genome. Front. Genet. 2019, 10, 275. [Google Scholar] [CrossRef] [PubMed]
  2. Wang, W.; Dong, H.; Sun, Y.; Cao, M.; Duan, Y.; Li, H.; Liu, Q.; Gu, Q.; Zhang, J. The efficacy of eugenol and tricaine methanesulphonate as anaesthetics for juvenile Chinese sea bass (Lateolabrax maculatus) during simulated transport. J. Appl. Ichthyol. 2019, 35, 551–557. [Google Scholar] [CrossRef]
  3. Parlapani, F.F.; Haroutounian, S.A.; Nychas, G.J.E.; Boziaris, I.S. Microbiological spoilage and volatiles production of gutted European sea bass stored under air and commercial modified atmosphere package at 2 °C. Food Microbiol. 2015, 50, 44–53. [Google Scholar] [CrossRef] [PubMed]
  4. Cai, L.; Cao, A.; Bai, F.; Li, J. Effect of ε-polylysine in combination with alginate coating treatment on physicochemical and microbial characteristics of Japanese sea bass (Lateolabrax japonicas) during refrigerated storage. LWT Food Sci. Technol. 2015, 62, 1053–1059. [Google Scholar] [CrossRef]
  5. Fukuma, Y.; Yamane, A.; Itoh, T.; Tsukamasa, Y.; Ando, M. Application of supercooling to long-term storage of fish meat. Fish. Sci. 2012, 78, 451–461. [Google Scholar] [CrossRef]
  6. Banerjee, R.; Maheswarappa, N.B. Superchilling of muscle foods: Potential alternative for chilling and freezing. Crit. Rev. Food Sci. 2017, 59, 1256–1263. [Google Scholar] [CrossRef] [PubMed]
  7. Kaale, L.D.; Eikevik, T.M.; Rustad, T.; Kolsaker, K. Superchilling of food: A review. J. Food Eng. 2011, 107, 141–146. [Google Scholar] [CrossRef]
  8. Stevik, A.M.; Claussen, I.C. Industrial superchilling, a practical approach. Procedia Food Sci. 2011, 1, 1265–1271. [Google Scholar] [CrossRef] [Green Version]
  9. Magnussen, O.M.; Haugland, A.; Hemmingsen, A.K.T.; Johansen, S.; Nordtvedt, T.S. Advances in superchilling of food-process characteristics and product quality. Trends Food Sci. Technol. 2008, 19, 418–424. [Google Scholar] [CrossRef]
  10. Cropotova, J.; Mozuraityte, R.; Standal, I.B.; Grøvlen, M.S.; Rustad, T. Superchilled, chilled and frozen storage of Atlantic mackerel (Scomber scombrus) fillets changes in texture, drip loss, protein solubility and oxidation. Int. J. Food Sci. Technol. 2019, 54, 2228–2235. [Google Scholar] [CrossRef]
  11. Luan, L.; Fu, S.; Yuan, C.; Ishimura, G.; Chen, S.; Chen, J.; Hu, Y. Combined effect of superchilling and tea polyphenols on the preservation quality of hairtail (Trichiurus haumela). Int. J. Food Prop. 2017, 20, S992–S1001. [Google Scholar] [CrossRef]
  12. Xu, Y.; Li, T.; Zhang, C.; Li, X.; Yi, S.; Li, J.; Sun, X. Protein degradation of olive flounder (Paralichthys olivaceus) muscle after postmortem superchilled and refrigerated storage. Int. J. Food Prop. 2018, 21, 1911–1922. [Google Scholar] [CrossRef]
  13. Tsironi, T.N.; Taoukis, P.S. Effect of storage temperature and osmotic pre-treatment with alternative solutes on the shelf-life of gilthead seabream (Sparus aurata) fillets. Aquac. Fish. 2017, 2, 39–47. [Google Scholar] [CrossRef]
  14. Socaciu, M.I.; Semeniuc, C.A.; Vodnar, D.C. Edible films and coatings for fresh fish packaging: Focus on quality changes and shelf-life extension. Coatings 2018, 8, 10. [Google Scholar] [CrossRef]
  15. Yu, D.; Wu, L.; Regenstein, J.M.; Jiang, Q.; Yang, F.; Xu, Y.; Xia, W. Recent advances in quality retention of non-frozen fish and fishery products: A review. Crit. Rev. Food Sci. Nutr. 2019, 3, 1–13. [Google Scholar] [CrossRef]
  16. Hosseini, S.F.; Gómez-Guillén, M.C. A state-of-the-art review on the elaboration of fish gelatin as bioactive packaging: Special emphasis on nanotechnology-based approaches. Trends Food Sci. Technol. 2018, 79, 125–135. [Google Scholar] [CrossRef]
  17. Talón, E.; Lampi, A.M.; Vargas, M.; Chiralt, A.; Jouppila, K.; González-Martínez, C. Encapsulation of eugenol by spray-drying using whey protein isolate or lecithin: Release kinetics, antioxidant and antimicrobial properties. Food Chem. 2019, 295, 588–598. [Google Scholar] [CrossRef]
  18. Sharifimehr, S.; Soltanizadeh, N.; Hossein Goli, S.A. Effects of edible coating containing nano-emulsion of Aloe vera and eugenol on the physicochemical properties of shrimp during cold storage. J. Sci. Food Agric. 2019, 99, 3604–3615. [Google Scholar] [CrossRef]
  19. Monteschio, J.O.; Vargas-Junior, F.M.; Almeida, F.L.; Pinto, L.A.D.M.; Kaneko, I.N.; Almeida, A.A.; Freitas, L.W.; Alves, S.P.; Bessa, R.J.; Prado, I.N. The effect of encapsulated active principles (eugenol, thymol and vanillin) and clove and rosemary essential oils on the structure, collagen content, chemical composition and fatty acid profile of Nellore heifers muscle. Meat Sci. 2019, 155, 27–35. [Google Scholar] [CrossRef]
  20. Talón, E.; Vargas, M.; Chiralt, A.; González-Martínez, C. Antioxidant starch-based films with encapsulated eugenol. Application to sunflower oil preservation. LWT Food Sci. Technol. 2019, 113, 108290. [Google Scholar] [CrossRef]
  21. Sun, X.; Guo, X.; Ji, M.; Wu, J.; Zhu, W.; Wang, J.; Cheng, C.; Chen, L.; Zhang, Q. Preservative effects of fish gelatin coating enriched with CUR/βCD emulsion on grass carp (Ctenopharyngodon idellus) fillets during storage at 4 °C. Food Chem. 2019, 272, 643–652. [Google Scholar] [CrossRef] [PubMed]
  22. Shao, Y.; Wu, C.; Wu, T.; Li, Y.; Chen, S.; Yuan, C.; Hu, Y. Eugenol-chitosan nanoemulsions by ultrasound-mediated emulsification: Formulation, characterization and antimicrobial activity. Carbohydr. Polym. 2018, 193, 144–152. [Google Scholar] [CrossRef] [PubMed]
  23. Kim, J.H.; Hong, W.S.; Oh, S.W. Effect of layer-by-layer antimicrobial edible coating of alginate and chitosan with grapefruit seed extract for shelf-life extension of shrimp (Litopenaeus vannamei) stored at 4 °C. Int. J. Biol. Macromol. 2018, 120, 1468–1473. [Google Scholar] [CrossRef]
  24. Li, N.; Shen, Y.; Liu, W.; Mei, J.; Xie, J. Low-field NMR and MRI to analyze the effect of edible coating incorporated with MAP on qualities of half-smooth tongue sole (Cynoglossus Semilaevis Gunther) fillets during refrigerated storage. Appl. Sci. 2018, 8, 1391. [Google Scholar] [CrossRef]
  25. Neira, L.M.; Agustinelli, S.P.; Ruseckaite, R.A.; Martucci, J.F. Shelf life extension of refrigerated breaded hake medallions packed into active edible fish gelatin films. Packag. Technol. Sci. 2019, 1–10. [Google Scholar] [CrossRef]
  26. Cheng, J.H.; Sun, D.W.; Pu, H.B.; Wang, Q.J.; Chen, Y.N. Suitability of hyperspectral imaging for rapid evaluation of thiobarbituric acid (TBA) value in grass carp (Ctenopharyngodon idella) fillet. Food Chem. 2015, 171, 258–265. [Google Scholar] [CrossRef] [PubMed]
  27. Li, N.; Liu, W.R.; Shen, Y.; Mei, J.; Xie, J. Coating effects of epsilon-polylysine and rosmarinic acid combined with chitosan on the storage quality of fresh half-smooth tongue sole (Cynoglossus semilaevis Gunther) fillets. Coatings 2019, 9, 273. [Google Scholar] [CrossRef]
  28. Abdelhedi, O.; Jridi, M.; Nasri, R.; Mora, L.; Toldrá, F.; Nasri, M. Rheological and structural properties of Hemiramphus far skin gelatin: Potential use as an active fish coating agent. Food Hydrocoll. 2019, 87, 331–341. [Google Scholar] [CrossRef]
  29. Remya, S.; Mohan, C.O.; Venkateshwarlu, G.; Sivaraman, G.K.; Ravishankar, C.N. Combined effect of O2 scavenger and antimicrobial film on shelf life of fresh cobia (Rachycentron canadum) fish steaks stored at 2 °C. Food Control 2017, 71, 71–78. [Google Scholar] [CrossRef]
  30. Dimitrijević, M.; Grković, N.; Bošković, M.; Baltić, M.Ž.; Dojčinović, S.; Karabasil, N.; Vasilev, D.; Teodorović, V. Inhibition of Listeria monocytogenes growth on vacuum packaged rainbow trout (Oncorhynchus mykiss) with carvacrol and eugenol. J. Food Saf. 2019, 39, e12553. [Google Scholar] [CrossRef]
  31. Vatavali, K.; Karakosta, L.; Nathanailides, C.; Georgantelis, D.; Kontominas, M.G. Combined effect of chitosan and oregano essential oil dip on the microbiological, chemical, and sensory attributes of red porgy (Pagrus pagrus) stored in ice. Food Bioprocess Technol. 2013, 6, 3510–3521. [Google Scholar] [CrossRef]
  32. Singh, S.; Lee, M.; Gaikwad, K.K.; Lee, Y.S. Antibacterial and amine scavenging properties of silver–silica composite for post-harvest storage of fresh fish. Food Bioprod. Process. 2018, 107, 61–69. [Google Scholar] [CrossRef]
  33. Wang, Z.; Hu, S.; Gao, Y.; Ye, C.; Wang, H. Effect of collagen-lysozyme coating on fresh-salmon fillets preservation. LWT Food Sci. Technol. 2017, 75, 59–64. [Google Scholar] [CrossRef]
  34. Cheng, J.H.; Sun, D.W.; Wei, Q. Enhancing visible and near-infrared hyperspectral imaging prediction of TVB-N level for fish fillet freshness evaluation by filtering optimal variables. Food Anal. Method 2017, 10, 1888–1898. [Google Scholar] [CrossRef]
  35. Volpe, M.G.; Siano, F.; Paolucci, M.; Sacco, A.; Sorrentino, A.; Malinconico, M.; Varricchio, E. Active edible coating effectiveness in shelf-life enhancement of trout (Oncorhynchusmykiss) fillets. LWT Food Sci. Technol. 2015, 60, 615–622. [Google Scholar] [CrossRef]
  36. Vilas, C.; Alonso, A.A.; Herrera, J.R.; Bernárdez, M.; García, M.R. A mathematical model to predict early quality attributes in hake during storage at low temperature. J. Food Eng. 2018, 222, 11–19. [Google Scholar] [CrossRef] [Green Version]
  37. Li, D.; Zhang, L.; Song, S.; Wang, Z.; Kong, C.; Luo, Y. The role of microorganisms in the degradation of adenosine triphosphate (ATP) in chill-stored common carp (Cyprinus carpio) fillets. Food Chem. 2017, 224, 347–352. [Google Scholar] [CrossRef] [PubMed]
  38. Cheng, J.H.; Sun, D.W.; Qu, J.H.; Pu, H.B.; Zhang, X.C.; Song, Z.; Chen, X.; Zhang, H. Developing a multispectral imaging for simultaneous prediction of freshness indicators during chemical spoilage of grass carp fish fillet. J. Food Eng. 2016, 182, 9–17. [Google Scholar] [CrossRef]
  39. Alsaggaf, M.S.; Moussa, S.H.; Tayel, A.A. Application of fungal chitosan incorporated with pomegranate peel extract as edible coating for microbiological, chemical and sensorial quality enhancement of Nile tilapia fillets. Int. J. Biol. Macromol. 2017, 99, 499–505. [Google Scholar] [CrossRef] [PubMed]
  40. Da Silva, S.B.; Ferreira, D.; Pintado, M.; Sarmento, B. Chitosan-based nanoparticles for rosmarinic acid ocular delivery-In vitro tests. Int. J. Biol. Macromol. 2016, 84, 112–120. [Google Scholar] [CrossRef] [PubMed]
  41. Hassoun, A.; Çoban, Ö.E. Essential oils for antimicrobial and antioxidant applications in fish and other seafood products. Trends Food Sci. Technol. 2017, 68, 26–36. [Google Scholar] [CrossRef]
  42. Khan, M.I.; Adrees, M.N.; Arshad, M.S.; Anjum, F.M.; Jo, C.; Sameen, A. Oxidative stability and quality characteristics of whey protein coated rohu (Labeo rohita) fillets. Lipids Health Dis. 2015, 14, 58. [Google Scholar] [CrossRef]
  43. Ojagh, S.M.; Rezaei, M.; Razavi, S.H.; Hosseini, S.M.H. Effect of chitosan coatings enriched with cinnamon oil on the quality of refrigerated rainbow trout. Food Chem. 2010, 120, 193–198. [Google Scholar] [CrossRef]
  44. Yu, D.; Xu, Y.; Regenstein, J.M.; Xia, W.; Yang, F.; Jiang, Q.; Wang, B. The effects of edible chitosan-based coatings on flavor quality of raw grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage. Food Chem. 2018, 242, 412–420. [Google Scholar] [CrossRef] [PubMed]
  45. Tan, X.; Qi, L.; Fan, F.; Guo, Z.; Wang, Z.; Song, W.; Du, M. Analysis of volatile compounds and nutritional properties of enzymatic hydrolysate of protein from cod bone. Food Chem. 2018, 264, 350–357. [Google Scholar] [CrossRef] [PubMed]
  46. Reza, M.S.; Bapary, M.A.J.; Ahasan, C.T.; Islam, M.N.; Kamal, M. Shelf life of several marine fish species of Bangladesh during ice storage. Int. J. Food Sci. Technol. 2009, 44, 1485–1494. [Google Scholar] [CrossRef]
  47. Albertos, I.; Martin-Diana, A.B.; Cullen, P.J.; Tiwari, B.K.; Ojha, S.K.; Bourke, P.; Rico, D. Shelf-life extension of herring (Clupea harengus) using in-package atmospheric plasma technology. Innov. Food Sci. Emerg. Technol. 2019, 53, 85–91. [Google Scholar] [CrossRef]
  48. Huang, L.; Cheng, S.; Song, Y.; Xia, K.; Xu, X.; Zhu, B.W.; Tan, M. Non-destructive analysis of caviar compositions using low-field nuclear magnetic resonance technique. J. Food Meas. Charact. 2017, 11, 621–628. [Google Scholar] [CrossRef]
  49. Sun, X.H.; Xiao, L.; Lan, W.Q.; Liu, S.C.; Wang, Q.; Yang, X.H.; Zhang, W.J.; Xie, J. Effects of temperature fluctuation on quality changes of large yellow croaker (Pseudosciaena crocea) with ice storage during logistics process. J. Food Process. Preserv. 2017, 42, e13505. [Google Scholar] [CrossRef]
  50. Wang, S.; Xiang, W.; Fan, H.; Xie, J.; Qian, Y.F. Technology, Study on the mobility of water and its correlation with the spoilage process of salmon (Salmo solar) stored at 0 and 4 °C by low-field nuclear magnetic resonance (LF NMR 1H). J. Food Sci. Technol. 2018, 55, 173–182. [Google Scholar] [CrossRef]
Figure 1. Changes in total viable count (a), H2S-producing bacteria (b), Pseudomonas spp. (c), and Psychrophilic counts (d) of Chinese seabass during superchilling storage at −0.9 °C. (CK: uncoated; G-βCD: gelatin-β-cyclodextrin coating without eugenol; G-βCD-0.075%E: gelatin-β-cyclodextrin coating with 0.075% eugenol; G-βCD-0.15%E: gelatin-β-cyclodextrin coating with 0.15% eugenol; G-βCD-0.3%E: gelatin-β-cyclodextrin coating with 0.3% eugenol).
Figure 1. Changes in total viable count (a), H2S-producing bacteria (b), Pseudomonas spp. (c), and Psychrophilic counts (d) of Chinese seabass during superchilling storage at −0.9 °C. (CK: uncoated; G-βCD: gelatin-β-cyclodextrin coating without eugenol; G-βCD-0.075%E: gelatin-β-cyclodextrin coating with 0.075% eugenol; G-βCD-0.15%E: gelatin-β-cyclodextrin coating with 0.15% eugenol; G-βCD-0.3%E: gelatin-β-cyclodextrin coating with 0.3% eugenol).
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Figure 2. Changes in pH values (a), total volatile basic nitrogen values (TVB-N, b), inosine 5′-monophosphate (IMP, c), hypoxanthine (Hx, d), K value (e), and Thiobarbituric acid (TBA, f) of Chinese seabass during superchilling storage at −0.9 °C. (CK: uncoated; G-βCD: gelatin-β-cyclodextrin coating without eugenol; G-βCD-0.075%E: gelatin-β-cyclodextrin coating with 0.075% eugenol; G-βCD-0.15%E: gelatin-β-cyclodextrin coating with 0.15% eugenol; G-βCD-0.3%E: gelatin-β-cyclodextrin coating with 0.3% eugenol).
Figure 2. Changes in pH values (a), total volatile basic nitrogen values (TVB-N, b), inosine 5′-monophosphate (IMP, c), hypoxanthine (Hx, d), K value (e), and Thiobarbituric acid (TBA, f) of Chinese seabass during superchilling storage at −0.9 °C. (CK: uncoated; G-βCD: gelatin-β-cyclodextrin coating without eugenol; G-βCD-0.075%E: gelatin-β-cyclodextrin coating with 0.075% eugenol; G-βCD-0.15%E: gelatin-β-cyclodextrin coating with 0.15% eugenol; G-βCD-0.3%E: gelatin-β-cyclodextrin coating with 0.3% eugenol).
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Figure 3. Changes in water distribution (a) and magnetic resonance imaging (b) of Chinese seabass during superchilling storage at −0.9 °C. (CK: uncoated; G-βCD: gelatin-β-cyclodextrin coating without eugenol; G-βCD-0.075%E: gelatin-β-cyclodextrin coating with 0.075% eugenol; G-βCD-0.15%E: gelatin-β-cyclodextrin coating with 0.15% eugenol; G-βCD-0.3%E: gelatin-β-cyclodextrin coating with 0.3% eugenol).
Figure 3. Changes in water distribution (a) and magnetic resonance imaging (b) of Chinese seabass during superchilling storage at −0.9 °C. (CK: uncoated; G-βCD: gelatin-β-cyclodextrin coating without eugenol; G-βCD-0.075%E: gelatin-β-cyclodextrin coating with 0.075% eugenol; G-βCD-0.15%E: gelatin-β-cyclodextrin coating with 0.15% eugenol; G-βCD-0.3%E: gelatin-β-cyclodextrin coating with 0.3% eugenol).
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Figure 4. Changes in organoleptic properties results (smell: a; color: b; mucus: c; muscular tissue: d; elasticty: e) of Chinese seabass during superchilling storage at −0.9 °C. (CK: uncoated; G-βCD: gelatin-β-cyclodextrin coating without eugenol; G-βCD-0.0%75E: gelatin-β-cyclodextrin coating with 0.075%eugenol; G-βCD-0.15%E: gelatin-β-cyclodextrin coating with 0.15% μL mL−1 eugenol; G-βCD-0.3%E: gelatin-β-cyclodextrin coating with 0.3% eugenol).
Figure 4. Changes in organoleptic properties results (smell: a; color: b; mucus: c; muscular tissue: d; elasticty: e) of Chinese seabass during superchilling storage at −0.9 °C. (CK: uncoated; G-βCD: gelatin-β-cyclodextrin coating without eugenol; G-βCD-0.0%75E: gelatin-β-cyclodextrin coating with 0.075%eugenol; G-βCD-0.15%E: gelatin-β-cyclodextrin coating with 0.15% μL mL−1 eugenol; G-βCD-0.3%E: gelatin-β-cyclodextrin coating with 0.3% eugenol).
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Table 1. Changes in FAAs content (mg/100 g) in different treated Chinese seabass samples during superchilling storage.
Table 1. Changes in FAAs content (mg/100 g) in different treated Chinese seabass samples during superchilling storage.
TimeGroupsFAAs
AspThrSerGluGlyAlaValMet
Day 02.36 ± 0.365.47 ± 0.557.28 ± 0.488.06 ± 0.4355.63 ± 0.5934.20 ± 0.324.21 ± 0.252.63 ± 0.17
Day 15CK9.82 ± 0.26a8.86 ± 0.56c9.50 ± 0.34c12.03 ± 0.53b75.80 ± 0.47d47.11 ± 0.47c6.16 ± 0.22c3.40 ± 0.20b
G-βCD9.68 ± 0.22a12.22 ± 0.71a13.94 ± 0.38a18.87 ± 0.95a82.56 ± 1.13c59.61 ± 1.43a10.24 ± 0.16a6.16 ± 0.26a
G-βCD-0.075%E8.90 ± 0.41b8.09 ± 0.65d10.56 ± 0.44b9.28 ± 0.49c84.28 ± 0.64c47.78 ± 0.75c6.05 ± 0.19c3.04 ± 0.16c
G-βCD-0.15%E8.49 ± 0.19c9.10 ± 0.53c9.15 ± 0.37c7.37 ± 0.38d96.87 ± 0.68b48.97 ± 0.83c7.43 ± 0.14b3.66 ± 0.43b
G-βCD-0.3%E7.75 ± 0.28d9.64 ± 0.42b13.86 ± 0.27a7.13 ± 0.33d104.98 ± 0.49a52.24 ± 0.28b7.00 ± 0.27bc3.45 ± 0.32b
Day 30CK2.64 ± 0.14a8.69 ± 0.43c10.17 ± 0.52c14.07 ± 0.47bc68.56 ± 0.63c41.42 ± 0.43d7.88 ± 0.57d4.55 ± 0.32c
G-βCD2.54 ± 0.75a17.28 ± 0.58a12.96 ± 0.59a27.50 ± 0.48a63.37 ± 0.67d58.44 ± 52a19.93 ± 0.73a11.25 ± 0.48a
G-βCD-0.075%E2.36 ± 0.35a10.47 ± 0.35b12.72 ± 0.0.93ab15.26 ± 0.45b81.34 ± 0.76bc54.75 ± 0.48bc10.06 ± 0.63b5.78 ± 0.37b
G-βCD-0.15%0E2.39 ± 0.18a11.06 ± 0.84b11.83 ± 0.62ab12.30 ± 0.44d82.22 ± 0.68b47.49 ± 0.42cd9.07 ± 0.595.51 ± 0.31b
G-βCD-0.3%E2.27 ± 0.32a10.19 ± 0.41bc11.69 ± 0.47b13.37 ± 0.46c86.06 ± 0.62a52.85 ± 0.48b10.25 ± 0.68b5.16 ± 0.35bc
IleLeuTyrPheLysHisArgProTotal
Day 02.60 ± 0.183.83 ± 0.291.20 ± 0.212.82 ± 0.2810.34 ± 0.3417.81 ± 0.462.76 ± 0.223.37 ± 0.27164.62 ± 4.37
Day 15CK3.81 ± 0.24c5.98 ± 0.32c2.13 ± 0.18b3.76 ± 0.23b9.59 ± 0.38c27.28 ± 0.77a3.80 ± 0.25d3.76 ± 0.28c232.81 ± 4.76c
G-βCD6.73 ± 0.75a11.64 ± 0.43a6.10 ± 0.36a7.33 ± 0.46a22.18 ± 0.44a26.94 ± 0.32a8.80 ± 0.63a7.28 ± 0.36a310.31 ± 5.84a
G-βCD-0.075%E3.63 ± 0.29c5.57 ± 0.28c1.94 ± 0.17b3.70 ± 0.26b18.24 ± 0.48b26.42 ± 0.53a4.62 ± 0.18c5.29 ± 0.23b247.45 ± 4.28c
G-βCD-0.15%E5.00 ± 0.88b7.66 ± 0.25b2.60 ± 0.23b4.21 ± 0.31b18.79 ± 0.37b21.50 ± 0.38b5.81 ± 0.35b5.10 ± 0.34b261.75 ± 4.98b
G-βCD-0.3%E4.53 ± 0.27b6.97 ± 0.31b2.30 ± 0.25b4.11 ± 0.27b22.37 ± 0.36a19.52 ± 0.53c6.01 ± 0.23b7.29 ± 0.26a279.20 ± 5.22b
Day 30CK4.35 ± 0.87d7.81 ± 0.34d4.79 ± 0.69b5.22 ± 0.48c22.81 ± 0.74c45.64 ± 0.83a6.40 ± 0.63b4.69 ± 0.74c259.69 ± 4.28c
G-βCD12.00 ± 1.32a22.40 ± 0.75a14.71 ± 1.06a12.72 ± 0.74a32.77 ± 0.97a35.62 ± 0.59b12.56 ± 01.22a11.49 ± 0.63a367.57 ± 5.74a
G-βCD-0.075%E5.90 ± 0.59bc9.64 ± 0.44c4.74 ± 0.64b6.52 ± 0.48b20.43 ± 0.56d36.64 ± 0.48b5.95 ± 0.49b6.78 ± 0.72b289.36 ± 4.92b
G-βCD-0.15%E5.28 ± 0.99c9.21 ± 0.63bc4.86 ± 0.65b5.75 ± 0.39bc26.42 ± 0.64b33.88 ± 1.30b5.82 ± 0.69b6.38 ± 0.83b279.51 ± 4.73bc
G-βCD-0.3%E6.46 ± 0.76b11.07 ± 0.48b5.16 ± 1.59b6.12 ± 0.57b27.08 ± 0.73b33.47 ± 0.88b6.17 ± 0.53b6.23 ± 0.77b293.62 ± 5.07b
Different larger case letters in same group from different day indicate a significant difference (p < 0.05).

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Zhou, Q.; Li, P.; Fang, S.; Liu, W.; Mei, J.; Xie, J. Preservative Effects of Gelatin Active Coating Enriched with Eugenol Emulsion on Chinese Seabass (Lateolabrax maculatus) during Superchilling (−0.9 °C) Storage. Coatings 2019, 9, 489. https://doi.org/10.3390/coatings9080489

AMA Style

Zhou Q, Li P, Fang S, Liu W, Mei J, Xie J. Preservative Effects of Gelatin Active Coating Enriched with Eugenol Emulsion on Chinese Seabass (Lateolabrax maculatus) during Superchilling (−0.9 °C) Storage. Coatings. 2019; 9(8):489. https://doi.org/10.3390/coatings9080489

Chicago/Turabian Style

Zhou, Qianqian, Peiyun Li, Shiyuan Fang, Wenru Liu, Jun Mei, and Jing Xie. 2019. "Preservative Effects of Gelatin Active Coating Enriched with Eugenol Emulsion on Chinese Seabass (Lateolabrax maculatus) during Superchilling (−0.9 °C) Storage" Coatings 9, no. 8: 489. https://doi.org/10.3390/coatings9080489

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