Abscisic Acid and Cytokinins Are Not Involved in the Regulation of Stomatal Conductance of Scots Pine Saplings during Post-Drought Recovery
Abstract
:1. Introduction
- -
- analysis of changes in ABA and CK contents in pine plants during water stress and recovery (Experiment 1);
- -
- analysis of the effects of exogenous CK treatment on stomatal conductance in pine plants (Experiment 2).
2. Materials and Methods
2.1. Plant Material
2.2. Experimental Design
2.2.1. Experiment 1
2.2.2. Experiment 2
- “Drought”—plants under continuous drought, which were not rewatered at Day 0 and thereafter.
- “Recovery”—plants rewatered to full soil capacity and sprayed with water (12.5 mL per plant) supplemented with 0.02% Silwet™ 408 as the surfactant.
- “Recovery + 0.1 BAP”—plants rewatered to full soil capacity and sprayed with 0.1 µM 6-benzylaminopurine (6-BAP) in water (12.5 mL per plant) + 0.02% Silwet™ 408.
- “Recovery + 1.0 BAP”—plants rewatered to full soil capacity and sprayed with 1.0 µM BAP in water (12.5 mL per plant) + 0.02% Silwet™ 408.
- “Recovery + 10 BAP”—plants rewatered to full soil capacity and sprayed with 10 µM BAP (12.5 mL per plant) + 0.02% Silwet™ 408.
- “Recovery + 100 BAP”—plants rewatered to full soil capacity and sprayed with 100 µM BAP (12.5 mL per plant) +0.02% Silwet™ 408.
2.3. Stomatal Conductance to Water Vapour
2.4. Leaf Water Potential (Ψleaf)
2.5. Absolute and Relative Needle Water Content
2.6. Analysis of Plant Hormones and Related Compounds
2.7. Statistical Analysis
3. Results
3.1. Changes in Needle Water Status in Water-Stressed and Recovering Plants (Experiment 1)
3.2. Changes in Hormonal Balance in Water-Stressed and Recovering Plants (Experiment 1)
3.3. Effects of Exogenous Cytokinin on Recovery of Needle Water Status (Experiment 2)
4. Discussion
4.1. ABA Accumulates during Water Stress and Decreases Rapidly in Recovering Plants
4.2. Dynamics of Cytokinins and CK/ABA Ratio Do Not Coincide with Recovery of Stomatal Conductance
4.3. Possible Reasons for Delayed Recovery of Stomatal Conductance
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zlobin, I.E.; Vankova, R.; Dobrev, P.I.; Gaudinova, A.; Kartashov, A.V.; Ivanov, Y.V.; Ivanova, A.I.; Kuznetsov, V.V. Abscisic Acid and Cytokinins Are Not Involved in the Regulation of Stomatal Conductance of Scots Pine Saplings during Post-Drought Recovery. Biomolecules 2023, 13, 523. https://doi.org/10.3390/biom13030523
Zlobin IE, Vankova R, Dobrev PI, Gaudinova A, Kartashov AV, Ivanov YV, Ivanova AI, Kuznetsov VV. Abscisic Acid and Cytokinins Are Not Involved in the Regulation of Stomatal Conductance of Scots Pine Saplings during Post-Drought Recovery. Biomolecules. 2023; 13(3):523. https://doi.org/10.3390/biom13030523
Chicago/Turabian StyleZlobin, Ilya E., Radomira Vankova, Petre I. Dobrev, Alena Gaudinova, Alexander V. Kartashov, Yury V. Ivanov, Alexandra I. Ivanova, and Vladimir V. Kuznetsov. 2023. "Abscisic Acid and Cytokinins Are Not Involved in the Regulation of Stomatal Conductance of Scots Pine Saplings during Post-Drought Recovery" Biomolecules 13, no. 3: 523. https://doi.org/10.3390/biom13030523