Label-Free LC-MS Profiling of Skeletal Muscle Reveals Heart-Type Fatty Acid Binding Protein as a Candidate Biomarker of Aerobic Capacity
Abstract
:1. Introduction
2. Experimental
3. Results
3.1. Physical and Physiological Characteristics of HCR and LCR Rats
3.2. LC-MS Profiling of Soluble Muscle Proteins
Description | Database ID | MOWSE (peptides) | Relative Abundance LCR | Relative Abundance HCR | Fold Diff. | p value |
---|---|---|---|---|---|---|
Respiratory chain | ||||||
Complex III | ||||||
Cytochrome b-c1 complex subunit 1 | QCR1 | 322 (11) | 109.87 ± 8.8 | 121.26 ± 5 | 1.10 | 0.0274 |
Cytochrome b-c1 complex subunit 2 | QCR2 | 459 (15) | 166.77 ± 10.6 | 182.25 ± 8.7 | 1.09 | 0.0290 |
Complex IV | ||||||
Cytochrome c oxidase subunit 2 | COX2 | 111 (4) | 50.93 ± 4.2 | 58.16 ± 5.9 | 1.14 | 0.0458 |
Cytochrome c oxidase subunit 4 isoform 1 | COX41 | 159 (5) | 68.78 ± 5 | 79.39 ± 7.3 | 1.15 | 0.0206 |
Complex V | ||||||
ATP synthase subunit beta | ATPB | 1185 (21) | 740.15 ± 66.1 | 843.71 ± 69.4 | 1.14 | 0.0326 |
ATP synthase subunit d | ATP5H | 64 (2) | 40.96 ± 2.8 | 47.47 ± 3.4 | 1.16 | 0.0081 |
ADP/ATP translocase 1 | ADT1 | 562 (10) | 457.55 ± 45.4 | 528.89 ± 40.5 | 1.16 | 0.0232 |
Voltage-dependent anion-selective channel protein 1 | VDAC1 | 412 (12) | 197.73 ± 13.1 | 221.68 ± 11.3 | 1.12 | 0.0111 |
Voltage-dependent anion selective channel protein 3 | VDAC3 | 53 (4) | 45.82 ± 3.7 | 52.94 ± 3.9 | 1.16 | 0.0137 |
Electron transfer flavoprotein subunit beta | ETFB | 279 (8) | 113.23 ± 8.6 | 128.03 ± 10.4 | 1.13 | 1.13 |
Fatty acid transport | ||||||
Fatty acid-binding protein, heart | FABPH | 349 (7) | 328.72 ± 62.3 | 504.97 ± 116.5 | 1.54 | 0.0064 |
Fatty acid-binding protein, adipocyte | FABP4 | 95 (4) | 48.06 ± 5.2 | 55.47 ± 5.7 | 1.15 | 0.0426 |
Methylmalonate-semialdehyde dehydrogenase | MMSA | 115 (5) | 55.59 ± 2 | 58.63 ± 5 | 1.05 | 0.0235 |
Dihydrolipoyl dehydrogenase | DLDH | 184 (6) | 57.83 ± 3.8 | 66.78 ± 4.9 | 1.15 | 0.0077 |
3-Ketoacyl-CoA thiolase | THIM | 396 (7) | 89.21 ± 10.6 | 111.88 ± 11.4 | 1.25 | 0.0071 |
Enoyl-CoA hydratase | ECHM | 76 (3) | 21.76 ± 2.2 | 25.97 ± 3.1 | 1.19 | 0.0245 |
3.3. SRM Verification of FABPH
4. Discussion
5. Conclusions
Supplementary Materials
Supplementary File 1Acknowledgments
Conflicts of Interest
References and Notes
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Malik, Z.A.; Cobley, J.N.; Morton, J.P.; Close, G.L.; Edwards, B.J.; Koch, L.G.; Britton, S.L.; Burniston, J.G. Label-Free LC-MS Profiling of Skeletal Muscle Reveals Heart-Type Fatty Acid Binding Protein as a Candidate Biomarker of Aerobic Capacity. Proteomes 2013, 1, 290-308. https://doi.org/10.3390/proteomes1030290
Malik ZA, Cobley JN, Morton JP, Close GL, Edwards BJ, Koch LG, Britton SL, Burniston JG. Label-Free LC-MS Profiling of Skeletal Muscle Reveals Heart-Type Fatty Acid Binding Protein as a Candidate Biomarker of Aerobic Capacity. Proteomes. 2013; 1(3):290-308. https://doi.org/10.3390/proteomes1030290
Chicago/Turabian StyleMalik, Zulezwan A., James N. Cobley, James P. Morton, Graeme L. Close, Ben J. Edwards, Lauren G. Koch, Steven L. Britton, and Jatin G. Burniston. 2013. "Label-Free LC-MS Profiling of Skeletal Muscle Reveals Heart-Type Fatty Acid Binding Protein as a Candidate Biomarker of Aerobic Capacity" Proteomes 1, no. 3: 290-308. https://doi.org/10.3390/proteomes1030290
APA StyleMalik, Z. A., Cobley, J. N., Morton, J. P., Close, G. L., Edwards, B. J., Koch, L. G., Britton, S. L., & Burniston, J. G. (2013). Label-Free LC-MS Profiling of Skeletal Muscle Reveals Heart-Type Fatty Acid Binding Protein as a Candidate Biomarker of Aerobic Capacity. Proteomes, 1(3), 290-308. https://doi.org/10.3390/proteomes1030290