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Bioengineering 2014, 1(1), 1-17; doi:10.3390/bioengineering1010001

Dynamic Experiments for Bioprocess Parameter Optimization with Extreme Halophilic Archaea

Research Division Biochemical Engineering, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1A 166-4, A-1060 Vienna, Austria
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Received: 10 September 2013 / Revised: 16 October 2013 / Accepted: 31 October 2013 / Published: 7 November 2013
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Abstract

The to-date studies on extreme halophiles were focused on shake flask cultivations. Bioreactor technology with quantitative approaches can offer a wide variety of biotechnological applications to exploit the special biochemical features of halophiles. Enabling industrial use of Haloferax mediterranei, finding the optima of cultivation parameters is of high interest. In general, process parameter optimizations were mainly carried out with laborious and time-consuming chemostat cultures. This work offers a faster alternative for process parameter optimization by applying temperature ramps and pH shifts on a halophilic continuous bioreactor culture. Although the hydraulic equilibrium in continuous culture is not reached along the ramps, the main effects on the activity from the dynamic studies can still be concluded. The results revealed that the optimal temperature range may be limited at the lower end by the activity of the primary metabolism pathways. At the higher end, the mass transfer of oxygen between the gaseous and the liquid phase can be limiting for microbial growth. pH was also shown to be a key parameter for avoiding overflow metabolism. The obtained experimental data were evaluated by clustering with multivariate data analyses. Showing the feasibility on a halophilic example, the presented dynamic methodology offers a tool for accelerating bioprocess development.
Keywords: dynamic ramps; process parameter optimization; bioprocess with extreme halophiles; acceleration bioprocess development dynamic ramps; process parameter optimization; bioprocess with extreme halophiles; acceleration bioprocess development
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Lorantfy, B.; Seyer, B.; Herwig, C. Dynamic Experiments for Bioprocess Parameter Optimization with Extreme Halophilic Archaea. Bioengineering 2014, 1, 1-17.

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