Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO2 via pH-Stat Jar Cultivation of an Engineered Hydrogen-Oxidizing Bacterium Cupriavidus necator
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains
2.2. Culture Medium
2.3. Subculture and Preculture
2.4. Recycled-Gas, Closed-Circuit Culture System (RGCC Culture System)
2.5. Conditions for the Main Culture
2.6. Analyses
3. Results
3.1. pH-Stat Jar Cultivation of Recombinant Strains
3.2. pH-Stat Jar Cultivation of MF01/pBPP-ccrMeJAc-emd with Supplemented Phosphate
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain | Relevant Marker | References or Resources |
---|---|---|
C. necator H16 | Wild type | DSM 428 |
C. necator MF01 | H16 derivative; ΔphaC::phaCNSDG, ΔphaA::bktB | [15] |
C. necator MF01ΔB1 | MF01 derivative; ΔphaB1 | [11] |
Plasmid | ||
pBPP | pBBR1-MCS2 derivative; PphaP1, TrrnB | [15] |
pBPP-ccrMeJ4a-emd | pBPP derivative; ccrMe, phaJ4a, emdMm | [11] |
pBPP-ccrMeJAc-emd | pBPP derivative; ccrMe, phaJAc, emdMm | [12] |
Strain/Plasmid | Dry Cell Mass (g·L−1) | PHA Content in Cells (w/w%) | Monomer Composition (mol%) | |
---|---|---|---|---|
3HB | 3HHx | |||
H16 (wild strain) | 44.05 | 78.5 | 100.0 | 0 |
MF01/pBPP-ccrMeJ4a-emd | 45.42 | 57.7 | 92.9 | 7.1 |
MF01ΔB1/pBPP-ccrMeJ4a-emd | 23.06 | 66.8 | 78.8 | 21.2 |
MF01/pBPP-ccrMeJAc-emd | 40.12 | 83.9 | 89.1 | 10.9 |
MF01ΔB1/pBPP-ccrMeJAc-emd | 26.70 | 75.5 | 90.9 | 9.10 |
Strain/Plasmid | Cultivation Time (h) | PHBHHx Concentration (g·L−1) | Productivity (g·L−1·h−1) |
---|---|---|---|
H16 (wild strain) | 120 | P(3HB) 34.58 | P(3HB) 0.288 |
MF01/pBPP-ccrMeJ4a-emd | 156 | 26.19 | 0.168 |
MF01ΔB1/pBPP-ccrMeJ4a-emd | 156 | 15.40 | 0.099 |
MF01/pBPP-ccrMeJAc-emd | 156 | 33.66 | 0.216 |
MF01ΔB1/pBPP-ccrMeJAc-emd | 156 | 20.16 | 0.129 |
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Tanaka, K.; Orita, I.; Fukui, T. Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO2 via pH-Stat Jar Cultivation of an Engineered Hydrogen-Oxidizing Bacterium Cupriavidus necator. Bioengineering 2023, 10, 1304. https://doi.org/10.3390/bioengineering10111304
Tanaka K, Orita I, Fukui T. Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO2 via pH-Stat Jar Cultivation of an Engineered Hydrogen-Oxidizing Bacterium Cupriavidus necator. Bioengineering. 2023; 10(11):1304. https://doi.org/10.3390/bioengineering10111304
Chicago/Turabian StyleTanaka, Kenji, Izumi Orita, and Toshiaki Fukui. 2023. "Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO2 via pH-Stat Jar Cultivation of an Engineered Hydrogen-Oxidizing Bacterium Cupriavidus necator" Bioengineering 10, no. 11: 1304. https://doi.org/10.3390/bioengineering10111304