Nutritional Strategies to Promote Bovine Oocyte Quality for In Vitro Embryo Production: Do They Really Work?

Round 1

Reviewer 1 Report

Nutritional strategies to promote bovine oocyte quality for in  vitro embryo production: do they really work?

The revision work is valid and I have a few suggestions.

The conclusion of the abstract should be in line with and similar to the overall conclusion of the paper.

The Keywords should be different from the title.

Line 32-The growing global human population predicted to surpass 9 billion by 2050 [1],  coupled with the projected decrease in food availability (e.g., red meat) due to climate  change [2]. I don't see how this literature relates to work in general. Especially the weather conditions.

Line 179-Although undernutrition does not always affect the ability of oocytes to form a blas- 179 tocyst [71], there is evidence indicating that a low plane of nutrition can decrease in vitro 180 [88,89] and vivo [90-92] oocyte developmental competence in domestic ruminants. It should generally indicate the species.

All table headings should be self-explanatory. example: Table 2, which is not.

Author Response

I thank the reviewer for taking the time to read this paper and provided useful suggestions/comments. Please find attached my response to your queries. 

Thank you

Author Response File: Author Response.pdf

Reviewer 2 Report

As summarized in this manuscript, nutritional status of oocyte donors plays critical role in the efficiency of in vitro embryo production (IVEP) in large livestock cattle. The author systematically reviewed the studies published in the last ten years of IVEP coupled with ovum pick-up (OPU) in cattle,  the impact of dietary intake of bovine oocyte donors on IVEP, the impact of fatty acid, mineral and vitamin supplementation of bovine oocyte donors on IVEP. The manuscript is well organized and written in native English based on the closely relevant literatures. 

Author Response

I thank the reviewer for taking the time to read this paper, and I appreciate the kind comments on the paper. 

Thank you