Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with Neochloris oleoabundans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microalgal Cultures
2.1.1. Pre-Inoculum Cultures and Control Conditions
2.1.2. Extractive Fermentation with Thermosensitive Polymers
2.2. Cell Viability in Extractive Fermentation
- [C]: Cell concentration (cells/mL);
- DF: Dilution factor;
- : Average number of cells counted per quadrant;
- Vf: Volume factor (10,000 mm3).
- Y: Log(N(T)/N0);
- N(T): Number of cells at a certain time;
- N0: Initial number of cells;
- a: Value of maximum growth;
- b: Constant (generated by software);
- c: Constant (generated by software);
- T: Time (h).
2.3. Generation of the In Situ Biphasic System for EPS Extraction
2.4. Exopolysaccharide and Biomass Quantification
- Y: yield;
- DE: dry weight of EPS;
- WB: wet weight of biomass.
- Y: yield;
- WB: wet weight of biomass;
- V: volume.
2.5. Extractive Fermentation with TP in a Semi-Continuous System
2.6. Statistical Analysis
3. Results
3.1. Extractive Fermentation and Cellular Viability
3.2. Generation of the Biphasic System
3.3. Extraction of EPS and Biomass
3.4. Extractive Fermentation in a Semi-Continuous System
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Condition A | Condition B | |||||||
---|---|---|---|---|---|---|---|---|
Fermentation | a | µmax | λ | G | a | µmax | λ | G |
Control | 1.49 | 0.03 | 33.46 | 18.92 | 0.98 | 0.00 | −2.55 | 99.84 |
EOPO 3900 g/mol 10% (w/v) | 1.29 | 0.02 | 71.01 | 30.21 | −2.45 | −0.01 | 249.10 | −35.64 |
EOPO 3900 g/mol 15% (w/v) | −0.06 | −0.05 | 71.21 | 13.14 | −2.39 | −0.15 | 253.62 | −4.50 |
EOPO 12,000 g/mol 10% (w/v) | 0.00 | - | 00.79 | - | 0.40 | 0.00 | 232.85 | 106.35 |
Conditions A | Conditions B | ||||||||
---|---|---|---|---|---|---|---|---|---|
Fermentation | VR | [TP] | YTP | [BP] | YBP | [TP] | YTP | [BP] | YBP |
Control | N/A | 13.63 ± 0.000 | 63.85 | N/A | N/A | 22.97 ± 0.002 | 21.54 | N/A | N/A |
EOPO 3900 g/mol 10% (w/v) | 13.67 | 11.33 ± 0.120 | 108.97 | - | - | 22.92 ± 0.000 | 44.62 | 22.31 ± 0.003 | 43.42 |
EOPO 3900 g/mol 15% (w/v) | 7 | 8.93 ± 0.094 | 113.56 | - | - | 22.89 ± 0.002 | 43.85 | 22.34 ± 0.005 | 42.81 |
EOPO 12,000 g/mol 10% (w/v) | 4 | - | - | - | - | 22.30 ± 0.000 | 30.24 | 21.95 ± 0.003 | 29.76 |
Condition A | Condition B | ||
---|---|---|---|
Fermentation | VR | YBP | YBP |
Control | N/A | 3.20 | 3.20 |
EOPO 3900 g/mol 10% (w/v) | 13.67 | 1.56 | 1.54 |
EOPO 3900 g/mol 15% (w/v) | 7.00 | 1.18 | 1.57 |
EOPO 12,000 g/mol 10% (w/v) | 4.00 | - | 2.21 |
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Garza-Chapa, A.P.; Ávila-Velasco, C.I.; González-Valdez, J.; Gómez-Loredo, A. Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with Neochloris oleoabundans. Fermentation 2024, 10, 130. https://doi.org/10.3390/fermentation10030130
Garza-Chapa AP, Ávila-Velasco CI, González-Valdez J, Gómez-Loredo A. Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with Neochloris oleoabundans. Fermentation. 2024; 10(3):130. https://doi.org/10.3390/fermentation10030130
Chicago/Turabian StyleGarza-Chapa, Ana Patricia, Carlos Iván Ávila-Velasco, José González-Valdez, and Alma Gómez-Loredo. 2024. "Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with Neochloris oleoabundans" Fermentation 10, no. 3: 130. https://doi.org/10.3390/fermentation10030130