Eriobotrya japonica Fermentation with Plant-Derived Lactiplantibacillus plantarum MSC-5T Ameliorates Antioxidant Activity in HEK293 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Identification of the LAB
2.2. Fermentation Condition of the MSC-5T Strain in EJ Extract
2.3. Cell Culture and Treatment
2.4. Measurement of Intracellular ROS Levels
2.5. Preparation of the Cell Extract
2.6. Quantification of Intracellular GSSH Levels
2.7. Quantification of Caspase-3 Activity
2.8. Extraction and Identification of an Antioxidant Compound Produced in the Fermented EJ Extract
2.9. Statistical Analysis
3. Results and Discussion
3.1. The Characterization of MSC-5T Strain
3.2. Effect of Fermented EJ Extracts on the Viability of HEK293 Cells under Oxidative Stress
3.3. Antioxidant and Antiproliferative Activity of Fermented EJ Extract
3.4. Effect of Fermented EJ Extract on GSH Homeostasis
3.5. Analysis of Antioxidant Components
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Unfermented EJ Extract | Fermented EJ Extract | ||
---|---|---|---|
Intracellular ROS (%) | H2O2 | 81 ± 4.1 | 70.5 ± 3.7 |
OTA | 93.3 ± 4.1 | 82.5 ± 3.7 | |
CTN | 80.3 ± 2.5 | 68.1 ± 5.2 | |
Intracellular Caspase-3 (%) | H2O2 | 103.1 ± 6.2 | 42.6 ± 3.0 |
OTA | 95 ± 5.1 | 68.9 ± 5.1 | |
CTN | 92.8 ± 2.3 | 83.6 ± 1.9 |
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Danshiitsoodol, N.; Inoue, Y.; Sugimoto, S.; Shakya, S.; Noda, M.; Sugiyama, M. Eriobotrya japonica Fermentation with Plant-Derived Lactiplantibacillus plantarum MSC-5T Ameliorates Antioxidant Activity in HEK293 Cells. Fermentation 2024, 10, 197. https://doi.org/10.3390/fermentation10040197
Danshiitsoodol N, Inoue Y, Sugimoto S, Shakya S, Noda M, Sugiyama M. Eriobotrya japonica Fermentation with Plant-Derived Lactiplantibacillus plantarum MSC-5T Ameliorates Antioxidant Activity in HEK293 Cells. Fermentation. 2024; 10(4):197. https://doi.org/10.3390/fermentation10040197
Chicago/Turabian StyleDanshiitsoodol, Narandalai, Yusuke Inoue, Sachiko Sugimoto, Shrijana Shakya, Masafumi Noda, and Masanori Sugiyama. 2024. "Eriobotrya japonica Fermentation with Plant-Derived Lactiplantibacillus plantarum MSC-5T Ameliorates Antioxidant Activity in HEK293 Cells" Fermentation 10, no. 4: 197. https://doi.org/10.3390/fermentation10040197