Development of Self-Assembled Biomimetic Nanoscale Collagen-like Peptide-Based Scaffolds for Tissue Engineering: An In Silico and Laboratory Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Computational Methods
2.1.1. I-Tasser
2.1.2. Replica Exchange Molecular Dynamics of (PAH)10
2.1.3. Replica Exchange Molecular Dynamics of Composite Scaffold
2.2. Laboratory Studies
2.2.1. Materials
2.2.2. Preparation of Polysaccharide-Peptide Conjugate
2.2.3. Self-Assembly of Polysaccharide-Peptide Conjugate and Conjugation with (FWKT)4
2.2.4. Incorporation of (PAH)10 and Choline Acetate by Layer-by-Layer Assembly
2.3. Characterization
2.3.1. Circular Dichroism (CD) Spectroscopy
2.3.2. Differential Scanning Calorimetry (DSC)
2.3.3. Rheology
2.3.4. Scanning Electron Microscopy (SEM)
2.3.5. Atomic Force Microscope (AFM)
2.3.6. Fourier Transform Infrared (FTIR) Spectroscopy
2.3.7. Dynamic Light Scattering (DLS)
2.3.8. Fluorescence Microscopy
2.4. Cell Studies
2.4.1. D Cell Culture
2.4.2. Cytotoxicity Studies
2.4.3. DNA Quantification Assay
2.4.4. Bioprinting
2.4.5. Inflammasome Assay
2.4.6. Cytoskeletal Studies
2.5. Statistical Analysis
3. Results and Discussion
3.1. REMD Simulations for Self-Assembly (Pro-Ala-His)10
3.2. I-Tasser
3.3. CD Studies
3.4. AFM Imaging and Dynamic Light Scattering Analysis
3.5. Formation of Multi-Layered Biomimetic Scaffold
REMD Simulations of Biomimetic Scaffold
3.6. Synthesis and Assembly of Biomimetic Scaffold
3.6.1. FTIR Analysis
3.6.2. Morphological Analysis of Biocomposite Scaffold
3.6.3. Thermal Analysis of Biocomposite Scaffold
3.6.4. Mechanical Properties
3.7. Cell Adhesion, Proliferation and Formation of Cell Scaffold Matrices
4. Conclusions
5. Future Goals
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Temperature (K) | Average RMSD (nm) |
---|---|
290 | 6.28 |
310 | 6.59 |
330 | 5.71 |
350 | 6.29 |
370 | 6.41 |
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Goncalves, B.G.; Heise, R.M.; Banerjee, I.A. Development of Self-Assembled Biomimetic Nanoscale Collagen-like Peptide-Based Scaffolds for Tissue Engineering: An In Silico and Laboratory Study. Biomimetics 2023, 8, 548. https://doi.org/10.3390/biomimetics8070548
Goncalves BG, Heise RM, Banerjee IA. Development of Self-Assembled Biomimetic Nanoscale Collagen-like Peptide-Based Scaffolds for Tissue Engineering: An In Silico and Laboratory Study. Biomimetics. 2023; 8(7):548. https://doi.org/10.3390/biomimetics8070548
Chicago/Turabian StyleGoncalves, Beatriz G., Ryan M. Heise, and Ipsita A. Banerjee. 2023. "Development of Self-Assembled Biomimetic Nanoscale Collagen-like Peptide-Based Scaffolds for Tissue Engineering: An In Silico and Laboratory Study" Biomimetics 8, no. 7: 548. https://doi.org/10.3390/biomimetics8070548