Combination of a Synthetic Bioceramic Associated with a Polydioxanone-Based Membrane as an Alternative to Autogenous Bone Grafting
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Sample Calculation
2.3. Surgical Procedure (Critical Calvaria Defect)
2.4. Distribution of Samples and Laboratory Processing
2.5. Microtomographic Analysis (Micro-CT)
2.6. Laser Confocal Microscopy Analysis
2.7. Gene Expression (RT-PCR)
2.8. Histometry Analysis (H.E)
2.9. Immunolabeling Analysis
2.10. Statistical Analysis
3. Results
3.1. Microtomographic Analysis (Micro-CT)
3.1.1. Bone Volume Percentage (BV.TV)
3.1.2. Trabecular Thickness (Tb.Th)
3.1.3. Trabecular Number (Tb.N)
3.1.4. Trabecular Separation (Tb.Sp)
3.1.5. Intersection Surface (i.S)
3.1.6. Total Porosity (Po.Tot)
3.2. Laser Confocal Microscopy Analysis
3.3. Gene Expression Analysis (RT-PCR)
3.3.1. Transcription Factor 2 (RUNX2)
3.3.2. Vascular Endothelial Growth Factor (VEGF)
3.3.3. Alkaline Phosphatase (ALP)
3.3.4. Osteocalcin (OCN)
3.3.5. Integrin Binding Sialoprotein (IBSP)
3.4. Histological Analysis
3.4.1. Bone Repair Process at 7 Days
3.4.2. Bone Repair Process at 30 Days
3.5. Immunolabeling Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Particulate Autogenous Bone + Plenum® Guide (AUTOPT+PG) | Plenum® Osshp + Plenum® Guide (PO+PG) | |
---|---|---|
BV.TV (%) | 49.29 ± 8.376 a | 56.26 ± 6.245 a |
Tb.Th (mm) | 0.28 ± 0.010 a | 0.21 ± 0.013 b |
Tb.N (mm) | 1.69 ± 0.242 a | 2.76 ± 0.471 b |
Tb.Sp (mm) | 0.32 ± 0.008 a | 0.20 ± 0.052 b |
i.S (mm2) | 25.04 ± 2.512 a | 26.76 ± 4.736 a |
Po.Tot (%) | 59.15 ± 6.370 a | 43.79 ± 6.285 b |
MAR (µm/day) | 5.99 ± 0.105 a | 7.16 ± 0.235 b |
Expression Genes | Particulate Autogenous Bone + Plenum® Guide (AUTOPT+PG) | Plenum® Osshp + Plenum® Guide (PO+PG) |
---|---|---|
RUNX2 | 1.06 ± 0.365 a | 0.30 ± 0.107 b |
VEGF | 0.99 ± 0.056 a | 0.50 ± 0.015 b |
ALP | 0.99 ± 0.034 a | 0.60 ± 0.172 b |
IBSP | 0.99 ± 0.042 a | 0.35 ± 0.094 b |
OCN | 0.99 ± 0.017 a | 0.95 ± 0.157 a |
Particulate Autogenous Bone + Plenum® Guide (AUTOPT+PG) | Plenum® Osshp + Plenum® Guide (PO+PG) | |
---|---|---|
RUNX2 | ++ | ++ |
OPN | + | ++ |
OCN | + | +++ |
TRAP | + | + |
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Frigério, P.B.; de Moura, J.; Pitol-Palin, L.; Monteiro, N.G.; Mourão, C.F.; Shibli, J.A.; Okamoto, R. Combination of a Synthetic Bioceramic Associated with a Polydioxanone-Based Membrane as an Alternative to Autogenous Bone Grafting. Biomimetics 2024, 9, 284. https://doi.org/10.3390/biomimetics9050284
Frigério PB, de Moura J, Pitol-Palin L, Monteiro NG, Mourão CF, Shibli JA, Okamoto R. Combination of a Synthetic Bioceramic Associated with a Polydioxanone-Based Membrane as an Alternative to Autogenous Bone Grafting. Biomimetics. 2024; 9(5):284. https://doi.org/10.3390/biomimetics9050284
Chicago/Turabian StyleFrigério, Paula Buzo, Juliana de Moura, Letícia Pitol-Palin, Naara Gabriela Monteiro, Carlos Fernando Mourão, Jamil Awad Shibli, and Roberta Okamoto. 2024. "Combination of a Synthetic Bioceramic Associated with a Polydioxanone-Based Membrane as an Alternative to Autogenous Bone Grafting" Biomimetics 9, no. 5: 284. https://doi.org/10.3390/biomimetics9050284