Preliminary Evaluation of Moniliformin as a Potential Threat for Teleosts
Abstract
:1. Introduction
2. Material and Methods
2.1. Ethics Statement on Animal Experiments
2.2. Experimental Model
2.3. Experimental Design
2.3.1. Assessment of the Effects on Operculum Mineralization in Zebrafish Larvae
2.3.2. Assessment of the Effects on the Incidence of Deformities in Zebrafish Larvae
2.3.3. Assessment of the Cytotoxic, Proliferative and Mineralogenic Effects In Vitro
2.3.4. RNA Extraction and Quantitative Real-Time PCR
2.4. Data Analysis
3. Results
3.1. Assessment of the Effects on Operculum Mineralization in Zebrafish Larvae
3.2. Assessment of the Effects on the Incidence of Deformities in Zebrafish Larvae
3.3. Assessment of the Cytotoxic, Proliferative and Mineralogenic Effects In Vitro
3.4. Assessment of the Effects on Gene Expression Levels in Zebrafish Larvae
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Genes | Sequence (5′-3′) | NCBI Accession # | |
---|---|---|---|
runx2b | Fw: | TCAGGAATGCCTCAGGGGTTATG | NM_212862 |
Rev: | CTTGCGGTGGGTTTGTGAATACT | ||
sp7 | Fw: | GCTAAGTCCAGGGCAGGCTCAG | NM_212863 |
Rev: | CAATGGCGTGAAATCAGGAGTGTAAC | ||
bglap | Fw: | TTTATAGGCGGCGATGATTCC | NM_001083857 |
Rev: | GAAGCGAACATGAAGAGTCTGACAGTCC | ||
bglapl | Fw: | CCAACTCCGCATCAGACTCCGCATCA | NM_001291889 |
Rev: | AGCAACACTCCGCTTCAGCAGCACAT | ||
col1a1a | Fw: | GCTTCATTGCCCAGCCACAGGA | NM_199214 |
Rev: | GCAGGGTTCTTCTTGGTGCCGTCT | ||
col10a1a | Fw: | CAGGAAGAGTTTGGCGGCTGT | NM_131292 |
Rev: | GACACGGCACGGTTCTGGTT | ||
col10a1a | Fw: | ATACCCAGTTCTTGTCAAAAGTCCA | NM_001083827 |
Rev: | GATCATAATGCTGCTCTGCGTT | ||
mmp13 | Fw: | TGTCAGTGGCAGAGGTGGATGACTC | NM_001290479 |
Rev: | CGCCACCAGGAACAGATTGTAATATTGAG | ||
bmp2b | Fw: | GAGGAACTTAGGAGACGACGGGAACGC | NM_131360 |
Rev: | TCTCGGGAATGAGTCCAACGGCAC | ||
eef1a1l1 | Fw: | TTGAGAAGAAAATCGGTGGTGCTG | NM_131263 |
Rev: | GGAACGGTGTGATTGAGGGAAATTC | ||
actb1 | Fw: | GATGCGGAAACTGGCAAAGG | NM_131031 |
Rev: | GAGGAGGGCAAAGTGGTAAACG | ||
rps18 | Fw: | AACACGAACATTGATGGAAGACG | NM_173234 |
Rev: | ATTAGCAAGGACCTGGCTGTATTT |
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Gonçalves, R.A.; Tarasco, M.; Schatzmayr, D.; Gavaia, P. Preliminary Evaluation of Moniliformin as a Potential Threat for Teleosts. Fishes 2018, 3, 4. https://doi.org/10.3390/fishes3010004
Gonçalves RA, Tarasco M, Schatzmayr D, Gavaia P. Preliminary Evaluation of Moniliformin as a Potential Threat for Teleosts. Fishes. 2018; 3(1):4. https://doi.org/10.3390/fishes3010004
Chicago/Turabian StyleGonçalves, Rui A., Marco Tarasco, Dian Schatzmayr, and Paulo Gavaia. 2018. "Preliminary Evaluation of Moniliformin as a Potential Threat for Teleosts" Fishes 3, no. 1: 4. https://doi.org/10.3390/fishes3010004