Survey of the Apoptotic Effect of Ginnalin A on Hep3b Human Hepatocellular Carcinoma Cell Line †
Department of Medical Biology, Meram Faculty of Medicine, Necmettin Erbakan University, Konya 42060, Turkey
*
Author to whom correspondence should be addressed.
†
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
Proceedings 2017, 1(10), 1064; https://doi.org/10.3390/proceedings1101064
Published: 15 November 2017
(This article belongs to the Proceedings of Proceedings of the 2nd International Conference on Natural Products for Cancer Prevention and Therapy)
Abstract
:Hepatocellular carcinoma is the third most common cause of cancer-related deaths worldwide. Ginnalin A (GA) is one of the most important phenolic compounds of maple syrup and its anticancer effect has been shown that in several cancer cell lines. In this study, objective was to investigate the apoptotic effect of GA in Hep3B human hepatocellular carcinoma cell line. Cell viability was determined by using XTT method after the treatment with GA. Total RNA was isolated with TRIzol Reagent in control and dose group. Expressions of important genes in apoptosis including CASP3, CASP8, CASP9, CYCS, FAS and P53 were evaluated by qPCR. IC50 dose of GA was found as 155 μM for 72h in Hep3B cells. According to the qPCR results, a significant increase in the expression of CASP3, CASP8, CASP9, CYCS and P53 genes was observed as 12.09, 10.14, 3.37, 16.15 and 4.15 folds, respectively. In conclusion, it is thought that GA demonstrates the apoptotic effect on Hep3B human hepatocellular carcinoma cell line. GA can be evaluated as an effective anticancer agent in hepatocellular carcinoma after further molecular and functional analysis.
Keywords: Apoptosis; Ginnalin A; Hep3B cell line
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MDPI and ACS Style
Özden, P.; Avcı, E.; Vural, H. Survey of the Apoptotic Effect of Ginnalin A on Hep3b Human Hepatocellular Carcinoma Cell Line. Proceedings 2017, 1, 1064. https://doi.org/10.3390/proceedings1101064
AMA Style
Özden P, Avcı E, Vural H. Survey of the Apoptotic Effect of Ginnalin A on Hep3b Human Hepatocellular Carcinoma Cell Line. Proceedings. 2017; 1(10):1064. https://doi.org/10.3390/proceedings1101064
Chicago/Turabian StyleÖzden, Pınar, Ebru Avcı, and Hasibe Vural. 2017. "Survey of the Apoptotic Effect of Ginnalin A on Hep3b Human Hepatocellular Carcinoma Cell Line" Proceedings 1, no. 10: 1064. https://doi.org/10.3390/proceedings1101064
