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Animals
Special Issues
Biomarkers for Assessing Animal Semen Quality
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Biomarkers for Assessing Animal Semen Quality

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Reproduction".

Deadline for manuscript submissions: 31 August 2024 | Viewed by 614

Special Issue Editors

Dr. Carolina Tamargo de Miguel

E-Mail Website
Guest Editor
Department of Animal Selection and Reproduction, The Regional Agri-Food Research and Development Service of Asturias (SERIDA), ES-33394 Gijón, Spain
Interests: semen quality; biomarkers; diagnostic markers; male fertility; ejaculate analysis; semen parameters; spermatozoa
Special Issues, Collections and Topics in MDPI journals
Dr. Rodrigo Muiño Otero

E-Mail Website
Guest Editor
Department of Animal Pathology/IBADER, Veterinary School, Campus Terra, University of Santiago de Compostela, 27002 Lugo, Galicia, Spain
Interests: cattle
Dr. Carlos Olegario Hidalgo Ordoñez

E-Mail Website
Guest Editor
Animal Selection and Reproduction Centre—SERIDA, Principado de Asturias, 33394 Gijón, Spain
Interests: semen analysis; biomarkers; animal reproduction; andrology; diagnostic markers; male fertility; ejacu-late analysis; fertility assessment
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Conventional semen analysis is subjective, time-consuming, and variable, while biomarker-based analysis using flow cytometry, in comparison, is a highly reproducible, quantitative, and fast way to measure thousands of spermatozoa per sample. Despite the publication of studies on biomarkers that can predict fertility with high accuracy and sensitivity, to date, no biomarker has been identified in semen or seminal plasma that can detect sub-fertile males. Further research on this topic is needed to determine a single, fast, simple, and reliable laboratory technique that can categorize males and semen doses into infertile, sub-fertile, fertile, and highly fertile. Achieving this objective would lead to an improvement in the reproductive efficiency of livestock farms in different animal species.

We encourage you to submit the latest and most advanced research articles on Biomarkers for Assessing Animal Semen Quality. Specifically, those papers that have determined a molecule or protein in semen or seminal plasma that can be correlated with fertility, and studies that determine a biomarker that can detect bulls or seminal doses with compromised fertility. Seminal biomarkers in bovine, porcine, equine, ovine, and caprine species will be accepted. These papers should aim to identify the most robust molecular biomarkers in sperm and seminal plasma for the diagnosis of male infertility, but systematic reviews and meta-analyses of the literature on biomarkers for assessing semen animal quality are also encouraged.

Dr. Carolina Tamargo de Miguel
Dr. Rodrigo Muiño Otero
Dr. Carlos Olegario Hidalgo Ordoñez
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • sperm
  • fertility
  • sub-fertile male
  • molecular biomarkers
  • seminal plasma

Published Papers (1 paper)

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Research

13 pages, 1561 KiB  
Article
ProAKAP4 as Indicator of Long-Lasting Motility Marker in Post-Thaw Conditions in Stallions
by Marta Dordas-Perpinyà, Iván Yánez-Ortiz, Nicolas Sergeant, Vincent Mevel, Jaime Catalán, Jean-François Bruyas, Jordi Miró and Lamia Briand-Amirat
Animals 2024, 14(9), 1264; https://doi.org/10.3390/ani14091264 - 23 Apr 2024
Viewed by 417
Abstract
ProAKAP4, a precursor of AKAP4 (A-kinase anchor protein) found in the flagellum of mammalian and non-mammalian spermatozoa, serves as a structural protein with established correlations to motility parameters across diverse species. This study aimed to determine the proAKAP4 level evolution in thawed stallion [...] Read more.
ProAKAP4, a precursor of AKAP4 (A-kinase anchor protein) found in the flagellum of mammalian and non-mammalian spermatozoa, serves as a structural protein with established correlations to motility parameters across diverse species. This study aimed to determine the proAKAP4 level evolution in thawed stallion semen over a 3 h period, examining its correlation with motility descriptors and mitochondrial membrane potential. Utilizing sixteen ejaculates from four French warmblood stallions, this study involved maintaining thawed samples at 37 °C for 3 h, conducting proAKAP4 enzyme-linked immunosorbent assays (ELISA), computer-assisted sperm analysis (CASA), and mitochondrial membrane potential by JC-1 probe and flow cytometry at 0, 1, and 3 h post-thawing. The findings indicate significant positive correlations (p ≤ 0.05) between proAKAP4 levels and sperm total or progressive motility at all time points analyzed. Spermatozoa velocity descriptors (VAP, VCL, VSL) and spermatozoa lateral head displacement (ALH) display positive correlations (p ≤ 0.05) with ProAKAP4 at the 0 h post-thawing. ProAKAP4 concentration exhibits no discernible difference between batches with or without a cryoprotectant. Notably, proAKAP4 consumption remains insignificant within the initial hour after thawing but becomes significant (p ≤ 0.05) between 1 and 3 h post-thawing. In summary, proAKAP4 demonstrates positive correlations with total and progressive motility in stallion semen for up to 3 h after thawing, albeit showing a noticeable decrease starting from the first hour post-thawing, indicating a progressive consumption as a result of spermatozoa motile activity. Full article
(This article belongs to the Special Issue Biomarkers for Assessing Animal Semen Quality)
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