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Animals
Special Issues
New Strategies for Improving Bovine Oocyte Competence
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New Strategies for Improving Bovine Oocyte Competence

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Reproduction".

Deadline for manuscript submissions: closed (19 July 2023) | Viewed by 1866

Special Issue Editors

Dr. Joaquim Fernando Moreira Da Silva

E-Mail Website
Guest Editor
Department of Animal Reproduction, Faculty of Agrarian Sciences and Environment, University of The Azores, IITAA, 9700-042 Angra do Heroísmo, Portugal
Interests: animal husbandry; animal physiology; assisted reproduction technologies; animal welfare
Dr. Mayra Anton Dib Saleh

E-Mail Website
Guest Editor
Faculty of Agrarian Sciences and Environment, University of the Azores, 9700-042 Angra do Heroísmo, Portugal
Interests: animal husbandry; animal physiology; animal welfare; immunomodulation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The nutrition, health and welfare of cattle influence female fertility and have a substantial impact on farm profitability; thus, the challenge for researchers is to focus on reliable approaches and technical improvements to oocyte competence.

The aim of this Special Issue based on female fertility is to demonstrate current progress or suggest future biotechnological solutions for oocyte competence. Furthermore, this Special Issue focuses on new aspects of the control of oocyte maturation leading to achievements in their competence, making their fertilization and subsequent embryonic development possible. Given that oocyte maturation is the first step towards successful in vitro embryo production, testing new culture systems will undoubtedly improve the yield and quality of the embryos produced.

Recent advances in in vitro technologies with particular interest in oocyte maturation and quality using endogenous or exogenous agents, thermoprotective molecules that alleviate heat-induced oocyte oxidative stress, organelle damage and apoptosis, cryopreservation and chilling procedures and validation of new methodologies applied to bovine female physiology will be fully discussed in this Special Issue.

Dr. Joaquim Fernando Moreira Da Silva
Dr. Mayra Anton Dib Saleh 
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Animals is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • assisted technologies
  • bovine reproduction
  • female physiology
  • fertility biomarkers
  • gene editing

Published Papers (1 paper)

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Research

14 pages, 3754 KiB  
Article
Alternative Culture Systems for Bovine Oocyte In Vitro Maturation: Liquid Marbles and Differentially Shaped 96-Well Plates
by Andrea Fernández-Montoro, Daniel Angel-Velez, Camilla Benedetti, Nima Azari-Dolatabad, Osvaldo Bogado Pascottini, Ann Van Soom and Krishna Chaitanya Pavani
Animals 2023, 13(10), 1635; https://doi.org/10.3390/ani13101635 - 14 May 2023
Viewed by 1517
Abstract
In vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the in vivo environment by in vitro conditions has been challenging. Until now, conventional two-dimensional (2D) systems have been used for in vitro maturation of bovine cumulus-oocytes-complexes (COCs). However, [...] Read more.
In vivo-matured oocytes exhibit higher developmental competence than those matured in vitro but mimicking the in vivo environment by in vitro conditions has been challenging. Until now, conventional two-dimensional (2D) systems have been used for in vitro maturation of bovine cumulus-oocytes-complexes (COCs). However, using such systems present certain limitations. Therefore, alternative low-cost methodologies may help to optimize oocyte in vitro maturation. Here, we used two different systems to culture COCs and evaluate their potential influence on embryo development and quality. In the first system, we used treated fumed silica particles to create a 3D microenvironment (liquid marbles; LM) to mature COCs. In the second system, we cultured COCs in 96-well plates with different dimensions (flat, ultra-low attachment round-bottom, and v-shaped 96-well plates). In both systems, the nuclear maturation rate remained similar to the control in 2D, showing that most oocytes reached metaphase II. However, the subsequent blastocyst rate remained lower in the liquid marble system compared with the 96-well plates and control 2D systems. Interestingly, a lower total cell number was found in the resulting embryos from both systems (LM and 96-well plates) compared with the control. In conclusion, oocytes matured in liquid marbles or 96-well plates showed no remarkable change in terms of meiotic resumption. None of the surface geometries influenced embryo development while oocyte maturation in liquid marbles led to reduced embryo development. These findings show that different geometry during maturation did not have a large impact on oocyte and embryo development. Lower embryo production after in vitro maturation in liquid marbles was probably detected because in vitro maturation was performed in serum-free medium, which makes oocytes more sensitive to possible toxic effects from the environment. Full article
(This article belongs to the Special Issue New Strategies for Improving Bovine Oocyte Competence)
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