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Biosensors
Special Issues
CRISPR-Powered Biosensing
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CRISPR-Powered Biosensing

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: closed (31 October 2023) | Viewed by 1827

Special Issue Editors

Dr. Zhiheng Xu

E-Mail Website
Guest Editor
Department of Industrial and Systems Engineering, Rochester Institute of Technology, Rochester, NY 14623, USA
Interests: 3D printing; molecular diagnostics; nucleic acid detection; biosensing
Prof. Dr. Xiong Ding

E-Mail Website
Guest Editor
Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China
Interests: molecular diagnostics; nucleic acid detection; biosensing
Special Issues, Collections and Topics in MDPI journals
Dr. Kun Yin

E-Mail Website
Guest Editor
School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
Interests: 3D printing; molecular diagnostics; nucleic acid detection; biosensing
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

CRISPR/Cas technology won the Nobel Prize in Chemistry in 2020 for its outstanding contribution to gene editing. In addition to its application in gene editing, a large literature shows that CRISPR/Cas technology has outstanding advantages in biosensing, with high accuracy, good specificity, and fast response. More importantly, CRISPR technology is promising not only in its application to nucleic acid detection but also in the detection of other kinds of biomarkers, including heavy metal ions, small molecules, peptides, and proteins, posing a great application potential for next-generation diagnostics, agriculture, and environmental monitoring. Therefore, this Special Issue aims to present cutting-edge fundamental and applied research activities in CRISPR-driven biosensing, showcasing innovative technologies, methods, and novel integrated devices for next-generation diagnostics, agriculture, and environmental monitoring. We invite submissions that will help to advance research in the field of CRISPR-driven biosensing technologies.

Dr. Zhiheng Xu
Prof. Dr. Xiong Ding
Dr. Kun Yin
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • DNA
  • RNA
  • biosensing
  • nucleic acid detection
  • diagnostics
  • Cas9
  • Cas12
  • Cas13
  • CRISPR-Cas

Published Papers (1 paper)

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Research

13 pages, 3047 KiB  
Article
A CRISPR-Cas12a-Based Diagnostic Method for Japanese Encephalitis Virus Genotypes I, III, and V
by Namki Kwak, Bum Ju Park and Yoon-Jae Song
Biosensors 2023, 13(8), 769; https://doi.org/10.3390/bios13080769 - 28 Jul 2023
Viewed by 1186
Abstract
The Japanese encephalitis virus (JEV) is prevalent in Asian countries, including Korea, Japan, China, Vietnam, and India. JEV is transmitted to humans by Culex mosquitoes. Despite extensive research efforts, no approved antiviral agents are currently available, although JE can be prevented by vaccination. [...] Read more.
The Japanese encephalitis virus (JEV) is prevalent in Asian countries, including Korea, Japan, China, Vietnam, and India. JEV is transmitted to humans by Culex mosquitoes. Despite extensive research efforts, no approved antiviral agents are currently available, although JE can be prevented by vaccination. DNA endonuclease-targeted CRISPR trans reporter (DETECTR) is a newly emerging CRISPR-Cas12a-based molecular diagnostic method combined with isothermal nucleic acid amplification. In this study, DETECTR with reverse transcription–recombinase polymerase amplification (RT-RPA) was effectively utilized for JEV diagnosis and detected down to 10 RNA copies for JEV genotype I (GI) and 1 × 102 copies for both GIII and GV, achieving similar sensitivity to RT-PCR while displaying no cross-reaction with other viruses. A one-tube, one-temperature format of DETECTR was further developed, and its efficiency compared with that of conventional DETECTR. Full article
(This article belongs to the Special Issue CRISPR-Powered Biosensing)
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