3D Printing for Point-of-Care In Vitro Diagnostic Devices

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (1 November 2022) | Viewed by 5809

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Mechanical Engineering and Applied Mechanics (MEAM), School of Engineering and Applied Science, University of Pennsylvania, Towne Building, 220 33rd Street, Philadelphia, PA 19104, USA
Interests: active Control of flow patterns; carbon nanopipettes and cellular probes; electrokinetics and dielectrophoresis; liquid cell electron microscopy (the nanoaquarium); magneto-hydrodynamics (MHD); microfluidic pumps, stirrers; microfluidics with low temperature co-Fired ceramic tapes (LTCC); microswimmers (C. elegans); nanowalkers (molecular motors); point of care diagnostics (lab on chip); energy storage and desalination
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Special Issue Information

Dear Colleagues,

The current COVID-19 pandemic has underscored the need for rapid development and implementation of POC (point of care) tests for detecting virus infection, suitable for a wide range of venues and users.Considerable progress and innovation in laboratory-based assays offer new benchtop methods for immunoassays and nucleic-acid-based tests, including isothermal amplification, novel reporters, multiplexing capability, and enhanced specificity and sensitivity. However, their translation to low-cost, portable and convenient devices for field use, including home testing, and use in non-traditional settings, such as pharmacies, schools, and workplaces, remains a largely unmet need. Notably, 3D printing offers new avenues and capabilities for rapid prototyping to explore and validate designs and approaches, such as simplified immunoassays, nucleic acid tests (including both with amplification and non-amplification), optical and electrochemical detection, paper-based microfluidics, integrated sample prep, adaptions to various sample types (nasal swabs, saliva, urine), non-instrumented and minimally instrumented devices, including chemical heating, smartphone platforms, and connection with the IoMT (internet of medical things) and 5G networks.

Dr. Michael G. Mauk
Guest Editor

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Published Papers (2 papers)

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Research

9 pages, 2584 KiB  
Communication
Selective Detection of an Infection Biomarker by an Osteo-Friend Scaffold: Development of a Multifunctional Artificial Bone Substitute
by Hye-In Kim, Naren Raja, Youngjun Choi, Jueun Kim, Aram Sung, Yeong-Jin Choi, Hui-suk Yun and Honghyun Park
Biosensors 2021, 11(12), 473; https://doi.org/10.3390/bios11120473 - 24 Nov 2021
Cited by 2 | Viewed by 1643
Abstract
Developments in three-dimensional (3D) printing technologies have led to many potential applications in various biomedical fields, especially artificial bone substitutes (ABSs). However, due to the characteristics of artificial materials, biocompatibility and infection remain issues. Here, multifunctional ABSs have been designed to overcome these [...] Read more.
Developments in three-dimensional (3D) printing technologies have led to many potential applications in various biomedical fields, especially artificial bone substitutes (ABSs). However, due to the characteristics of artificial materials, biocompatibility and infection remain issues. Here, multifunctional ABSs have been designed to overcome these issues by the inclusion of a biochemical modality that allows simultaneous detection of an infection biomarker by osteo-friend 3D scaffolds. The developed multifunctional scaffolds consist of calcium-deficient hydroxyapatite (CDHA), which has a similar geometric structure and chemical composition to human bone, and gold nanoparticles (Au NPs), which assists osteogenesis and modulates the fluorescence of labels in their microenvironment. The Au NPs were subsequently conjugated with fluorescent dye-labeled probe DNA, which allowed selective interaction with a specific target biomarker, and the fluorescent signal of the dye was temporally quenched by the Au NP-derived Förster resonance energy transfer (FRET). When the probe DNA unfolded to bind to the target biomarker, the fluorescence signal was recovered due to the increased distance between the dye and Au NPs. To demonstrate this sensing mechanism, a microbial oligonucleotide was selected as a target biomarker. Consequently, the multifunctional scaffold simultaneously facilitated osteogenic proliferation and the detection of the infection biomarker. Full article
(This article belongs to the Special Issue 3D Printing for Point-of-Care In Vitro Diagnostic Devices)
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10 pages, 1831 KiB  
Article
Paper-Based Airborne Bacteria Collection and DNA Extraction Kit
by Youngung Seok, Joonseok Lee and Min-Gon Kim
Biosensors 2021, 11(10), 375; https://doi.org/10.3390/bios11100375 - 07 Oct 2021
Cited by 5 | Viewed by 3426
Abstract
The critical risk from airborne infectious diseases, bio-weapons, and harmful bacteria is currently the highest it has ever been in human history. The requirement for monitoring airborne pathogens has gradually increased to defend against bioterrorism or prevent pandemics, especially via simple and low-cost [...] Read more.
The critical risk from airborne infectious diseases, bio-weapons, and harmful bacteria is currently the highest it has ever been in human history. The requirement for monitoring airborne pathogens has gradually increased to defend against bioterrorism or prevent pandemics, especially via simple and low-cost platforms which can be applied in resource-limited settings. Here, we developed a paper-based airborne bacteria collection and DNA extraction kit suitable for simple application with minimal instruments. Airborne sample collection and DNA extraction for PCR analysis were integrated in the paper kit. We created an easy-to-use paper-based air monitoring system using 3D printing technology combined with an air pump. The operation time of the entire process, comprising air sampling, bacterial cell lysis, purification and concentration of DNA, and elution of the DNA analyte, was within 20 min. All the investigations and optimum settings were tested in a custom-designed closed cabinet system. In the fabricated cabinet system, the paper kit operated effectively at a temperature of 25–35 °C and 30–70% relative humidity for air containing 10–106 CFU Staphylococcus aureus. This paper kit could be applied for simple, rapid, and cost-effective airborne pathogen monitoring. Full article
(This article belongs to the Special Issue 3D Printing for Point-of-Care In Vitro Diagnostic Devices)
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