Special Issue "New and Old Technologies for Generation of Microarrays"
A special issue of Microarrays (ISSN 2076-3905).
Deadline for manuscript submissions: closed (31 March 2015)
Dr. Günter Roth
Microarrays have been the first method of choice for highly parallel DNA and RNA analysis as well as in molecular interaction studies. Even though Next Generation Sequencing (NGS) is believed to soon be able to count each RNA copy in a single cell; microarrays are still irreplaceable, especially in the field of proteins, antibodies and small molecules. Nevertheless the increasing demand in throughput, molecular purity, robustness and effective production leads to improvement of the old techniques of microarray generation and to innovative ideas of ‘making’ microarrays in completely new ways. Therefore ‘the simple act’ of Microarray Generation is the focus of this Special Issue “Microarray Generation—Old Paths and New Ways”. It will highlight the old and successful paths such as light-synthesis, spot-synthesis and outline future improvements to the reader, but also offer a glimpse into what is emerging at the moment and what is yet to come, i.e., what new possibilities may become available in the not too distant future. You are invited to present new ways of microarray generation, but also to provide proof that the old ‘paths’ still bear potential for vast improvements.
With best regards,
Dr. Günter Roth
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. Papers will be published continuously (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are refereed through a peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microarrays is an international peer-reviewed Open Access quarterly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 350 CHF (Swiss Francs). English correction and/or formatting fees of 250 CHF (Swiss Francs) will be charged in certain cases for those articles accepted for publication that require extensive additional formatting and/or English corrections.
- microarray generation
- DNA synthesis
- RNA synthesis
- protein synthesis
- microarray copying
- light synthesis
- spot synthesis
- microarray printing
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Label Detection and Label-free Detection for Protein Microarrays
Authors: Amir Syahir1, Kenji Usui2,*, Kin-ya Tomizaki3, Kotaro Kajikawa4, Hisakazu Mihara5,*
Affiliations: 1Department of Biochemistry, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, Serdang 43400, Malaysia.
2Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, 650-0047, Japan.
3Department of Materials Chemistry, Ryukoku University, Seta, Otsu 520-2194, Japan.
4Department of Electronics and Applied Physics Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Yokohama 226-8502, Japan.
5Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan. E-Mails: email@example.com
Abstract: Microarray has gone through many innovative developments in the recent decades. In this review, we focus on the development of protein detection methods embedded in the technology. Early works on microarray have utilized many useful chromophores and versatile biochemical techniques dominated the high-throughput illumination. Recently the realization of label-free techniques integrating into the microarray has been greatly advanced by the combination of knowledge in material sciences, computational design, and nanofabrication. These rapidly growing new techniques are aiming at providing data without the intervention of any label molecules. Here we present a brief overview of this remarkable innovation from both perspectives of label and label-free techniques in transducing nano-biological events.
Title: Aptamer microarrays – current status and future prospects
Authors: Martin Witt, Johanna Walter and Frank Stahl
Affiliation: Institut für Technische Chemie, Callinstr. 5, 30167 Hannover, Germany
Abstract: Biological research requires fast genome, proteome and transcriptome analysis technologies. Here, microarray technologies are state of the art. Since protein microarrays are limited in many experimental set-ups (e. g. multiplexing, detection of toxic targets or small molecules including organic compounds, etc.), aptamer microarrays provide alternative possibilities to circumvent these limitations.
In this article we review the latest developments in aptamer microarray technology. We discuss similarities and differences between DNA-, protein and aptamer microarrays and shed light on the post synthesis immobilization of aptamers with the corresponding effects on the microarray performance. Finally we highlight current limitations and future prospects of aptamer microarray technology.
Title: Reverse Phase Protein Array - quantitative assessment of multiple biomarkers in biopsies for clinical use
Authors: Stefanie Boellner and Karl-Friedrich Becker
Affiliations: Technische Universität München, Institut für Pathologie, Trogerstrasse 18, 81675 München
E-Mails: firstname.lastname@example.org, email@example.com
Abstract: Reverse phase protein arrays (RPPA) represent a very promising high-throughput technology to monitor changes in protein levels over time, before and after treatment, between disease and non-disease states and between responders and non-responders. This array format allows quantification of one protein or phospho-protein in multiple samples under the same experimental conditions at the same time. Moreover, it is suited for signal transduction profiling of small numbers of cultured cells or cells isolated from human biopsies, including formalin fixed and paraffin embedded (FFPE) tissues. After protein extraction, each patient sample is arrayed in duplicates on nitrocellulose-coated slides in a miniature dilution curve. Validated antibodies are used to detect the proteins of interest. Thus, each analyte/antibody combination can be analysed in the linear dynamic range. Current aspects of the RPPA technology, including dilution curves, spotting, controls, signal detection, antibody validation, and calculation of protein levels are addressed.