Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Pathogenic Microorganisms 3.0

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Medical Microbiology".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 1008

Special Issue Editor

Special Issue Information

Dear Colleagues,

This Special Issue is the continuation of our previous Special Issues, “Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Pathogenic Microorganisms”, “Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Pathogenic Microorganisms 2.0”.

The identification of pathogenic microorganisms for diagnostic purposes has undergone a radical change due to the introduction in clinical microbiology laboratories of Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS). The unquestionable rapidity, sensitivity, and reliability of MALDI-TOF MS is also accompanied by its versatility. The commercial systems available for the identification of bacteria and fungi can be borrowed for alternative uses through the intervention of the researchers, such as the identification of microorganisms different from those recognized by the systems, the identification of viruses, the execution of antimicrobial susceptibility testing, etc. This Special Issue aims to present a collection of articles providing a reliable picture of both the traditional and alternative uses of MALDI-TOF MS in the clinical microbiology laboratory, allowing the readers to have a summary of the potential applications of MALDI-TOF MS and stimulate them to identify new ones.

Prof. Dr. Adriana Calderaro
Guest Editor

Manuscript Submission Information

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Keywords

  • MALDI-TOF MS
  • diagnosis
  • microorganism identification
  • bacteria
  • viruses
  • parasites
  • fungi
  • antimicrobial susceptibility testing

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Published Papers (1 paper)

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Research

10 pages, 248 KiB  
Article
Evaluation of Autof MS2600 and MBT Smart MALDI-TOF MS Systems for Routine Identification of Clinical Bacteria and Yeasts
by Elena De Carolis, Vittorio Ivagnes, Carlotta Magrì, Benedetta Falasca, Teresa Spanu and Maurizio Sanguinetti
Microorganisms 2024, 12(2), 382; https://doi.org/10.3390/microorganisms12020382 - 13 Feb 2024
Viewed by 852
Abstract
The identification of microorganisms at the species level has always constituted a diagnostic challenge for clinical microbiology laboratories. The aim of the present study has been the evaluation in a real-time assay of the performance of Autobio in comparison with the Bruker mass [...] Read more.
The identification of microorganisms at the species level has always constituted a diagnostic challenge for clinical microbiology laboratories. The aim of the present study has been the evaluation in a real-time assay of the performance of Autobio in comparison with the Bruker mass spectrometry system for the identification of bacteria and yeasts. A total of 535 bacteria and yeast were tested in parallel with the two systems by direct smear or fast formic acid extraction for bacteria and yeasts, respectively. Discordant results were verified by 16S, ITS rRNA or specific gene sequencing. Beyond giving comparable results for bacteria with respect to the MBT smart system, Autof MS2600 mass spectrometer provided excellent accuracy for the identification of yeast species of clinical interest. Full article
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