Special Issue "Advances in Genome Engineering"

A special issue of Methods and Protocols (ISSN 2409-9279).

Deadline for manuscript submissions: 31 May 2018

Special Issue Editor

Guest Editor
Dr. Philip Hublitz

Head of Genome Engineering Facility, MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, Headington, OX3 9DS, UK
Website | E-Mail
Interests: Generation of mouse models, recombineering technologies, BAC-transgenesis, CRISPR/Cas9 technology, homologous recombination, NHEJ pathways, dCas9/mRNA-guided screens and imaging approaches, regulation of transcription processes, control of epigenetic processes

Special Issue Information

Dear colleagues,

With the emergence of the CRISPR/Cas9 genome-engineering platform an unprecedented variety of novel approaches and possibilities in the generation of cellular model systems has been made available, and many new exciting developments are unveiled on a regular basis.

The current Special Issue aims to provide a forum to (i) discuss the latest technical developments in the fields of general genome engineering technologies, (ii) focus on the establishment of cell-based model systems and on the development of animal models by CRISPR/Cas9 (or similar genome engineering tools), and (iii) give an overview about the current state of art of technology development and novel approaches using genome engineering technologies.

Due to the rapid development of the field this Special Issue invariably will not be able to cover all aspects. However, we aim to give a deep technical insight into the most recent evolution, latest trends and refinements in genomic engineering that is available today.

Dr. Philip Hublitz
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Methods and Protocols is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) is waived for well-prepared manuscripts submitted to this issue. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.



Keywords

  • CRISPR/Cas9

  • HDR/NHEJ Pathways

  • Base Editors

  • CRISPR-ON/OFF

  • Arrayed and Pooled sgRNA-Screening

  • Cas9 Efficiency and Fidelity

  • CRISPR/Cas9 Targeting Strategies

  • Model Generation

Published Papers (1 paper)

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Open AccessProtocol Generating CRISPR/Cas9-Derived Mutant Mice by Zygote Cytoplasmic Injection Using an Automatic Microinjector
Methods Protoc. 2018, 1(1), 5; doi:10.3390/mps1010005
Received: 20 November 2017 / Revised: 4 January 2018 / Accepted: 4 January 2018 / Published: 12 January 2018
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Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) assisted generation of mutant animals has become the method of choice for the elucidation of gene function in development and disease due to the shortened timelines for generation of a desired mutant, the ease of
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Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) assisted generation of mutant animals has become the method of choice for the elucidation of gene function in development and disease due to the shortened timelines for generation of a desired mutant, the ease of producing materials in comparison to other methodologies (such as embryonic stem cells, ESCs) and the ability to simultaneously target multiple genes in one injection session. Here we describe a step by step protocol, from preparation of materials through to injection and validation of a cytoplasmic injection, which can be used to generate CRISPR mutants. This can be accomplished from start of injection to completion within 2–4 h with high survival and developmental rates of injected zygotes and offers significant advantages over pronuclear and other previously described methodologies for microinjection. Full article
(This article belongs to the Special Issue Advances in Genome Engineering)
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