Design and Applications of Optical Microscopes

A special issue of Photonics (ISSN 2304-6732).

Deadline for manuscript submissions: 20 August 2024 | Viewed by 396

Special Issue Editors

Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an 710119, China
Interests: light field regulation; scattering medium; wavefront regulation; memory effect; digital holography

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Guest Editor
Shandong Provincial Engineering and Technical Center of Light Manipulations & Shandong Provincial Key Laboratory of Optics and Photonic Device, School of Physics and Electronics, Shandong Normal University, Jinan 250358, China
Interests: optical microscopes; light field regulation through complex media; construction and application of a novel structured light field
Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an 710119, China
Interests: optical microscopy; deep learning; compressive sensing; computational imaging; biomedical imaging
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Special Issue Information

Dear Colleagues,

As a commonly employed tool in life science and biomedical research, optical microscopy possesses a number of advantages, such as a high resolution, high sensitivity with a wealth of contrast mechanisms, and low damage impacts on the samples. Various optical system designs have promoted great advances in optical imaging, and structural and functional imaging could now be achieved at a high time–space scale.

For application in life science exploration, timely disease diagnosis and superior treatment, optical microscopy has been developed, on the one hand, for achieving deeper analysis with high temporal and spatial resolutions. On the other hand, optical microscopy has been designed to be more controllable, compact and smart for use. Controlling both the illumination and portion of the detection system have been established to enhance the imaging capacity. With the rapid development of optoelectronic technologies, metasurfaces, fiber optics, computing imaging and artificial intelligence, the performance of optical microscopy has been expanded.

This Special Issue focuses on original state-of-the-art research on optical microscopy and its applications. Both original research articles and reviews are welcome. Research areas may include (but are not limited to) the following:

  1. Confocal microscopy;
  2. Adaptive optics in optical microscopy;
  3. Fiber-based endoscopy;
  4. Metasurfaces for microscopy;
  5. Spatial light modulation and PSF engineering for microscopy

Dr. Runze Li
Dr. Qian Zhao
Dr. Chen Bai
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Photonics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • optical imaging
  • microscopy
  • confocal
  • adaptive optics
  • fiber optics
  • metasurfaces
  • PSF engineering

Published Papers (1 paper)

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Research

16 pages, 3165 KiB  
Article
Far-Field Super-Resolution Optical Microscopy for Nanostructures in a Reflective Substrate
by Aiqin Zhang, Kunyang Li, Guorong Guan, Haowen Liang, Xiangsheng Xie and Jianying Zhou
Photonics 2024, 11(5), 409; https://doi.org/10.3390/photonics11050409 (registering DOI) - 27 Apr 2024
Viewed by 92
Abstract
The resolution of an optical microscope is determined by the overall point spread function of the system. When examining structures significantly smaller than the wavelength of light, the contribution of the background or surrounding environment can profoundly affect the point spread function. This [...] Read more.
The resolution of an optical microscope is determined by the overall point spread function of the system. When examining structures significantly smaller than the wavelength of light, the contribution of the background or surrounding environment can profoundly affect the point spread function. This research delves into the impact of reflective planar substrate structures on the system’s resolution. We establish a comprehensive forward imaging model for a reflection-type confocal laser scanning optical microscope, incorporating vector field manipulation to image densely packed nanoparticle clusters. Both theoretical and experimental findings indicate that the substrate causes an interference effect between the background field and the scattered field from the nanoparticles, markedly enhancing the overall spatial resolution. The integration of vector field manipulation with an interferometric scattering approach results in superior spatial resolution for imaging isolated particles and densely distributed nanoscale particle clusters even with deep subwavelength gaps as small as 20 nm between them. However, the method still struggles to resolve nanoparticles positioned directly next to each other without any gap, necessitating further work to enhance the resolving ability. This may involve techniques like deconvolution or machine learning-based post-processing methods. Full article
(This article belongs to the Special Issue Design and Applications of Optical Microscopes)
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