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Portable Biosensors for Rapid Detection
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Portable Biosensors for Rapid Detection

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: 20 July 2024 | Viewed by 1651

Special Issue Editors

Dr. Georgia Paraskevi Nikoleli

E-Mail Website
Guest Editor
Department of Biomedical Sciences, Faculty of Health and Care Sciences, University of West Attica, Egaleo Park Campus, 12243 Athens, Greece
Interests: nanosensors; analytical and environmental chemistry; nanotechnology
Prof. Dr. Dimitrios P. Nikolelis

E-Mail Website
Guest Editor
Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, 15771 Athens, Greece
Interests: biosensors; analytical and environmental chemistry; nanotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In a new era of mobile healthcare and point-of-care testing, the need for fast, portable, and efficient diagnostic tools is growing. Biosensors offer many advantages over traditional laboratory assays in terms of sensitivity, accuracy, and rapid response and potential portability. They are now considered a promising alternative tool and one of the most accessible rapid detection methods in relation to field use and end users.

The importance of portable biosensors has been widely recognized, and many studies have explored their potential applications in food safety, clinical diagnostics, personalized medicine, global health, environmental monitoring, etc. This Special Issue aims to analyze recent trends in portable biosensors, especially in connection with nanotechnology, genetic engineering, microfluidics, MEMS, and mobile devices. Topics include, but are not limited to, the following:

  • portable biosensors;
  • paper-based biosensors;
  • droplet-based biosensors;
  • nucleic acid based biosensors;
  • aptamer-based biosensors;
  • biosensors based on antigenic gold/silver nanoparticles;
  • electrochemical biosensors;
  • optical biosensors;
  • surface plasmon resonance.

Dr. Georgia Paraskevi Nikoleli
Prof. Dr. Dimitrios P. Nikolelis
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (2 papers)

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Research

23 pages, 12160 KiB  
Article
Research on the Flow Characteristics and Reaction Mechanisms of Lateral Flow Immunoassay under Non-Uniform Flow
by Xuyan Zhao, Yuan Zhang, Qunfeng Niu, Li Wang, Chenglong Xing, Qiao Wang and Hui Bao
Sensors 2024, 24(6), 1989; https://doi.org/10.3390/s24061989 - 20 Mar 2024
Viewed by 454
Abstract
Lateral flow immunoassay (LFIA) is extensively utilized for point-of-care testing due to its ease of operation, cost-effectiveness, and swift results. This study investigates the flow dynamics and reaction mechanisms in LFIA by developing a three-dimensional model using the Richards equation and porous media [...] Read more.
Lateral flow immunoassay (LFIA) is extensively utilized for point-of-care testing due to its ease of operation, cost-effectiveness, and swift results. This study investigates the flow dynamics and reaction mechanisms in LFIA by developing a three-dimensional model using the Richards equation and porous media transport, and employing numerical simulations through the finite element method. The study delves into the transport and diffusion behaviors of each reaction component in both sandwich LFIA and competitive LFIA under non-uniform flow conditions. Additionally, the impact of various parameters (such as reporter particle concentration, initial capture probe concentrations for the T-line and C-line, and reaction rate constants) on LFIA performance is analyzed. The findings reveal that, in sandwich LFIA, optimizing parameters like increasing reporter particle concentration and initial capture probe concentration for the T-line, as well as adjusting reaction rate constants, can effectively enhance detection sensitivity and broaden the working range. Conversely, in competitive LFIA, the effects are inverse. This model offers valuable insights for the design and enhancement of LFIA assays. Full article
(This article belongs to the Special Issue Portable Biosensors for Rapid Detection)
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16 pages, 4166 KiB  
Article
Fast Thermocycling in Custom Microfluidic Cartridge for Rapid Single-Molecule Droplet PCR
by Hirokazu Takahara, Hayato Tanaka and Masahiko Hashimoto
Sensors 2023, 23(24), 9884; https://doi.org/10.3390/s23249884 - 17 Dec 2023
Viewed by 777
Abstract
The microfluidic droplet polymerase chain reaction (PCR), which enables simultaneous DNA amplification in numerous droplets, has led to the discovery of various applications that were previously deemed unattainable. Decades ago, it was demonstrated that the temperature holding periods at the denaturation and annealing [...] Read more.
The microfluidic droplet polymerase chain reaction (PCR), which enables simultaneous DNA amplification in numerous droplets, has led to the discovery of various applications that were previously deemed unattainable. Decades ago, it was demonstrated that the temperature holding periods at the denaturation and annealing stages in thermal cycles for PCR amplification could be essentially eliminated if a rapid change of temperature for an entire PCR mixture was achieved. Microfluidic devices facilitating the application of such fast thermocycling protocols have significantly reduced the time required for PCR. However, in microfluidic droplet PCR, ensuring successful amplification from single molecules within droplets has limited studies on accelerating assays through fast thermocycling. Our developed microfluidic cartridge, distinguished for its convenience in executing single-molecule droplet PCR with common laboratory equipment, features droplets positioned on a thin glass slide. We hypothesized that applying fast thermocycling to this cartridge would achieve single-molecule droplet PCR amplification. Indeed, the application of this fast protocol demonstrated successful amplification in just 22 min for 30 cycles (40 s/cycle). This breakthrough is noteworthy for its potential to expedite microfluidic droplet PCR assays, ensuring efficient single-molecule amplification within a remarkably short timeframe. Full article
(This article belongs to the Special Issue Portable Biosensors for Rapid Detection)
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