Triterpenoid Contents and Anti-Inflammatory Properties of the Methanol Extracts of Ligustrum Species Leaves
Abstract
:1. Introduction
2. Results and Discussion
2.1. Analgesic activity of methanol extract from Ligustrum plants leaves in mice
Groups | Dose (g/kg) | Average writhing | Percentages of protection |
---|---|---|---|
VEH | 42.3 ± 1.9 | - | |
L. pricei | 0.1 | 42.2 ± 2.4 | 0 |
0.25 | 28.0 ± 0.9** | 33.8 | |
1 | 17.9± 1.9*** | 57.7 | |
L. sinense | 0.1 | 43.0 ± 1.1 | -1.7 |
0.25 | 30.8 ± 2.0** | 27.2 | |
1 | 19.3 ± 1.9*** | 54.4 | |
L. lucidum | 0.1 | 39.4 ± 4.2 | 6.9 |
0.25 | 31.3 ± 3.6* | 26 | |
1 | 25.9 ± 2.6** | 38.8 | |
ASA | 0.3 | 24.2 ± 2.1** | 42.8 |
2.2. Anti-inflammatory activity of methanol extract from the Ligustrum plants leaves in rats
2.3. Effects of the methanol extract from LP leaves on the microvascular permeability in rats
2.4. The cyclooxygenase-2 inhibiting activities of methanol extract from Ligustrum plants leaves in vitro
2.5. Triterpenoid contents of methanol extract from Ligustrum plants leaves
Plants | Yield (%) | Amyrin (μg/g) | Betulin (μg/g) | Betulinic acid (μg/g) | Lupeol (μg/g) | Oleanolic acid (μg/g) | Ursolic acid (μg/g) |
---|---|---|---|---|---|---|---|
L. pricei | 16.34 | 3782.81 ± 38.83* | - | 1877.89 ± 5.82*** | 3765.78 ± 61.69* | 380.21 ± 15.63** | 1070.76 ± 5.90** |
L. sinense | 28.74 | 2031.93 ± 24.31 | - | 313.43 ± 7.53 | 1983.26 ± 36.71 | 205.12 ± 0.30 | 680.25 ± 14.21 |
L. lucidum | 41.77 | 603.86 ± 5.99*** | 623.63 ± 3.32 | 472.26 ± 1.72** | 629.68 ± 11.06* | 957.69 ± 4.81*** | 3412.53 ± 6.84*** |
3. Experimental
3.1. Preparation of plant extracts and drugs
3.2. Animals
3.3. Acetic acid-induced abdominal writhing response in mice
3.4. Formalin-induced licking response in mice
3.5. Carrageenan-induced paw edema in rats
3.6. Microvascular permeability test in rats
3.7. Cyclooxygenase-2 inhibiting activities assay in vitro
3.8. Determination of triterpenoids by HPLC-PDA
3.9. Statistical analysis
4. Conclusions
Acknowledgements
References
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Wu, C.-R.; Hseu, Y.-C.; Lien, J.-C.; Lin, L.-W.; Lin, Y.-T.; Ching, H. Triterpenoid Contents and Anti-Inflammatory Properties of the Methanol Extracts of Ligustrum Species Leaves. Molecules 2011, 16, 1-15. https://doi.org/10.3390/molecules16010001
Wu C-R, Hseu Y-C, Lien J-C, Lin L-W, Lin Y-T, Ching H. Triterpenoid Contents and Anti-Inflammatory Properties of the Methanol Extracts of Ligustrum Species Leaves. Molecules. 2011; 16(1):1-15. https://doi.org/10.3390/molecules16010001
Chicago/Turabian StyleWu, Chi-Rei, You-Cheng Hseu, Jin-Cherng Lien, Li-Wei Lin, Yung-Ta Lin, and Hui Ching. 2011. "Triterpenoid Contents and Anti-Inflammatory Properties of the Methanol Extracts of Ligustrum Species Leaves" Molecules 16, no. 1: 1-15. https://doi.org/10.3390/molecules16010001