Reactive Oxygen Species-Induced Impairment of Endothelium-Dependent Relaxations in Rat Aortic Rings: Protection by Methanolic Extracts of Phoebe grandis
Abstract
:1. Introduction
2. Results and Discussion
2.1. tBuOOH-induced intracellular oxidative stress
2.2. Effect of MPG extract on vascular relaxations
2.3. Detection of superoxide anion
2.4. Discussion
3. Experimental
3.1. Preparation of MPG extract
3.2. Cell based intracellular anti-oxidant assay
3.3. Preparation of aortic rings
3.4. Pharmacological studies
3.5. Measurement of superoxide anions
3.6. Calculations and statistical analysis
3.7. Chemicals and drugs
4. Conclusions
Acknowledgement
References
- Balakumar, P.; Chakkarwar, V.A.; Krishan, P.; Singh, M. Vascular endothelial dysfunction: A tug of war in diabetic nephropathy? Biomed. Pharmacother. 2009, 63, 171–179. [Google Scholar] [CrossRef] [PubMed]
- Stehouwer, C.D. Endothelial dysfunction in diabetes nephropathy: State of the art and potential significance for non diabetic renal disease. Nephrol. Dial. Transplant. 2004, 19, 177–181. [Google Scholar] [CrossRef] [PubMed]
- Calles-Escandon, J.; Cipolla, M. Diabetes and Endothelial dysfunction: A Clinical Perspective. Endocrine Rev. 2001, 22, 36–52. [Google Scholar] [CrossRef] [PubMed]
- Berry, C.; Brosnan, M.J.; Fennell, J.; Hamilton, C.A.; Dominiczak, A.F. Oxidative stress and vascular damage in hypertension. Curr. Opin. Nephrol. Hypertens. 2001, 10, 247–255. [Google Scholar] [CrossRef] [PubMed]
- Mukhtar, M.R.; Martin, M.T.; Domansky, M.; Rais, M.; Hadi, A.H.; Awang, K. Phoebegrandines A and B, proaporphine-tryptamine dimers, from Phoebe grandis. Phytochemistry 1997, 45, 1543–1546. [Google Scholar] [CrossRef]
- Mukhtar, M.R.; Aziz, A.N.; Thomas, N.F.; Hadi, A.H.; Litaudon, M.; Awang, K. Grandine A, a new proaporphine alkaloid from the bark of Phoebe grandis. Molecules 2009, 14, 1227–1233. [Google Scholar] [CrossRef] [PubMed]
- O’Brien, P.; Carrasco-Pozo, C.; Speisky, H. Boldine and its antioxidant or health promoting properties. Chem. Biol. Interact. 2006, 159, 1–17. [Google Scholar] [CrossRef] [PubMed]
- Zhao, Q.; Zhao, Y.; Wang, K. Antinociceptive and free radical scavenging activities of alkaloids isolated from Lindera angustifolia. J. Ethnopharm. 2006, 106, 408–413. [Google Scholar] [CrossRef] [PubMed]
- Ajay, M.; Achike, F.I.; Mustafa, A.M.; Mustafa, M.R. Effect of quercetin on altered vascular reactivity in aortas from streptozotocin-induced diabetic rats. Diabetes Res. Clin. Pract. 2006, 73, 1–7. [Google Scholar] [CrossRef] [PubMed]
- Nakagawa, T.; Yokozawa, T. Direct scavenging of nitric oxide and superoxide by green tea. Food Chem. Toxicol. 2002, 40, 1745–1750. [Google Scholar] [CrossRef]
- Romero, M.; Jimenez, R.; Sanchez, M.; Lopez-Sepulveda, R.; Zarzeulo, M.J.; O’Valle, F.; Zarzuelo, A.; Pérez-Vizcaíno, F.; Duarte, J. Quercetin inhibits vascular superoxide production induced by endothelin-1: Role of NADPH oxidase, uncoupled eNOS and PKC. Arteriosclerosis 2009, 202, 58–67. [Google Scholar] [CrossRef] [PubMed]
- Vera, R.; Sanchez, M.; Galista, M.; Villar, I.C.; Jimenez, R.; Zarzuelo, A.; Pérez-Vizcaíno, F.; Duarte, J. Chronic administration of genistein improves endothelial dysfunction in spontaneously hypertensive rats: involvement of eNOS, caveolin and calmodulin expression and NADPH oxidase activity. Clin. Sci. 2007, 112, 183–191. [Google Scholar] [CrossRef] [PubMed]
- Cai, H.; Harrison, D.G. Endothelial Dysfunction in Cardiovascular Diseases: The Role of Oxidant Stress. Circ. Res. 2000, 87, 840–844. [Google Scholar] [CrossRef] [PubMed]
- Brandes, R.P.; Barton, M.; Philippens, K.M.; Schweitzer, G.; Mugge, A. Endothelial-derived superoxide anions in pig coronary arteries: evidence from lucigen chemiluminescence and histochemical techniques. J. Physiol. (Lond). 1997, 500, 331–341. [Google Scholar] [CrossRef]
- Didion, S.P.; Faraci, F.M. Effects of NADH and NADPH on superoxide levels and cerebral vascular tone. Am. J. Physiol. Heart Circ. Physiol. 2002, 282, H688–H695. [Google Scholar] [CrossRef] [PubMed]
- Lund, D.D.; Faraci, M.M.; Miller, F.R., Jr.; Heistad, D.D. Gene transfer of endothelial nitric oxide synthase improves relaxation of carotid arteries from diabetic rabbits. Circulation 2000, 101, 1027–1033. [Google Scholar] [CrossRef] [PubMed]
- Murad, F. Cyclic guanosine monophosphate as a mediator of vasodilatation. J. Clin. Invest. 1986, 78, 1–5. [Google Scholar] [CrossRef] [PubMed]
- Machha, A.; Mustafa, M.R. Chronic treatment with flavonoids prevents endothelial dysfunction in spontaneously hypertensive rat aorta. J. Cardiovasc. Pharmacol. 2005, 46, 36–40. [Google Scholar] [CrossRef] [PubMed]
- Achike, F.I.; Kwan, C.Y. Nitric oxide, human diseases and herbal products that affect the nitric oxide signalling pathway. Clin. Exp. Pharmacol. Physiol. 2003, 30, 605–615. [Google Scholar] [CrossRef] [PubMed]
- Schmeda-Hirschmann, G.; Rodriquez, T.A.; Theoduloz, C.; Astudillo, S.L.; Feresin, G.E.; Tapia, A. Free-radical scavengers and antioxidants from Peumus boldus Mol. ("Boldo"). Free Rad. Res. 2003, 37, 447–452. [Google Scholar] [CrossRef]
- Marklund, S.; Marklund, G. Involvement of superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 1974, 47, 469–474. [Google Scholar] [CrossRef] [PubMed]
- Stolk, J.; Hiltermann, T.J.; Dijkman, J.H.; Verhoeven, A.J. Characteristics of the inhibition of NADPH oxidase activation in neutrophils by apocynin, a methoxy-substituted catechol. Am. J. Resp. Cell Mol. Biol. 1994, 11, 95–102. [Google Scholar] [CrossRef] [PubMed]
- Kim, Y.A.; Kong, C.S.; Um, Y.R.; Lee, J.I.; Nam, T.J.; Seo, Y. Antioxidant efficacy of extracts from variety of seaweeds in a cellular system. Ocean Sci. J. 2008, 43, 31–37. [Google Scholar] [CrossRef]
- Chan, E.C.; Drummond, G.R.; Woodman, O.L. 3’, 4’-dihydroxyflavonol enhances nitric oxide bioavailability and improves vascular function after ischemia and reperfusion injury in the rat. J. Cardiovasc. Pharmacol. 2003, 42, 727–735. [Google Scholar] [CrossRef] [PubMed]
Sample Availability: Samples of the Phoebe grandis (Nees) Merr. (Lauraceae) stem bark are available from the authors. |
ACh | SNP | |||
---|---|---|---|---|
pEC50(M) | Rmax (%) | pEC50(M) | Rmax (%) | |
Control | -7.29 ± 0.13 | 93.36 ± 3.83 | -8.01 ± 0.11 | 105.09 ± 3.87 |
0.5 μg/mL extract | -7.49 ± 0.19 | 92.96 ± 2.93 | -8.36 ± 0.28 | 95.33 ± 1.83 |
5 μg/mL extract | -7.42 ± 0.16 | 91.02 ± 4.54 | -8.40 ± 0.19 | 93.63 ± 3.19 |
50 μg/mL extract | -6.94 ± 0.34 | 88.31 ± 2.36 | -8.23 ± 0.11 | 107.02 ± 4.69 |
β-NADH (300μM) | -6.99 ± 0.16 | 66.64 ± 1.25 * | -7.52 ± 0.28 | 98.67 ± 3.94 |
β-NADH ± 0.5 μg/mL extract | -7.34 ± 0.27 | 91.30 ± 2.49 | -8.53 ± 0.13 | 105.21 ± 3.70 |
β-NADH ± SOD (50 IU/mL) | -7.48 ± 0.18 | 84.00 ± 6.72 | -8.35 ± 0.13 | 96.33 ± 4.02 |
© 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Share and Cite
Yeh-Siang, L.; Subramaniam, G.; Hadi, A.H.A.; Murugan, D.; Mustafa, M.R. Reactive Oxygen Species-Induced Impairment of Endothelium-Dependent Relaxations in Rat Aortic Rings: Protection by Methanolic Extracts of Phoebe grandis. Molecules 2011, 16, 2990-3000. https://doi.org/10.3390/molecules16042990
Yeh-Siang L, Subramaniam G, Hadi AHA, Murugan D, Mustafa MR. Reactive Oxygen Species-Induced Impairment of Endothelium-Dependent Relaxations in Rat Aortic Rings: Protection by Methanolic Extracts of Phoebe grandis. Molecules. 2011; 16(4):2990-3000. https://doi.org/10.3390/molecules16042990
Chicago/Turabian StyleYeh-Siang, Lau, Gopal Subramaniam, A. Hamid A. Hadi, Dharmani Murugan, and Mohd Rais Mustafa. 2011. "Reactive Oxygen Species-Induced Impairment of Endothelium-Dependent Relaxations in Rat Aortic Rings: Protection by Methanolic Extracts of Phoebe grandis" Molecules 16, no. 4: 2990-3000. https://doi.org/10.3390/molecules16042990