Neuroprotective Effects of Vanillyl Alcohol in Gastrodia elata Blume Through Suppression of Oxidative Stress and Anti-Apoptotic Activity in Toxin-Induced Dopaminergic MN9D Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of GE on Cell Viability in MN9D Cells
2.2. Effect of Vanillyl Alcohol on Cell Viability in MN9D Cells
2.3. Free Radical Scavenging Activities of Vanillyl Alcohol by ESR
2.4. Vanillyl Alcohol Affects the Expression of Bcl-2 and Bax in MPP+-Treated Cells
2.5. Vanillyl Alcohol Suppresses MPP+-Induced PARP Proteolysis
3. Experimental
3.1. Materials
3.2. Preparation of GE
3.3. Cell Culture and Treatments
3.4. Assessment of Cell Viability
3.5. Isolation of Total RNA and Expression Analysis for Bax and Bcl-2 mRNA
3.6. Immunoblot Analysis for Cleaved PARP
3.7. Flow Cytometric Detection of Apoptotic Cells
3.8. Measurement of Free Radical Scavenging Activity
3.9. Measurement of Intracellular Reactive Oxygen Species (ROS)
3.10. Statistical Analysis
4. Conclusions
Acknowledgements
References and Notes
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Sample Availability: Samples of the compounds are available from the authors. |
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Kim, I.S.; Choi, D.-K.; Jung, H.J. Neuroprotective Effects of Vanillyl Alcohol in Gastrodia elata Blume Through Suppression of Oxidative Stress and Anti-Apoptotic Activity in Toxin-Induced Dopaminergic MN9D Cells. Molecules 2011, 16, 5349-5361. https://doi.org/10.3390/molecules16075349
Kim IS, Choi D-K, Jung HJ. Neuroprotective Effects of Vanillyl Alcohol in Gastrodia elata Blume Through Suppression of Oxidative Stress and Anti-Apoptotic Activity in Toxin-Induced Dopaminergic MN9D Cells. Molecules. 2011; 16(7):5349-5361. https://doi.org/10.3390/molecules16075349
Chicago/Turabian StyleKim, In Su, Dong-Kug Choi, and Hun Jong Jung. 2011. "Neuroprotective Effects of Vanillyl Alcohol in Gastrodia elata Blume Through Suppression of Oxidative Stress and Anti-Apoptotic Activity in Toxin-Induced Dopaminergic MN9D Cells" Molecules 16, no. 7: 5349-5361. https://doi.org/10.3390/molecules16075349