Extraction and Characterization of Chitin from the Beetle Holotrichia parallela Motschulsky
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chitin Extraction
Moisture (%) | Ash (%) | Nitrogen (%) a | |
---|---|---|---|
Dried raw materials | 3.63 ± 0.04 | 5.53 ± 0.23 | 11.29 ± 0.03 |
Beetle chitin | 7.12 ± 0.16 | 2.20 ± 0.13 | 6.33 ± 0.06 |
Shrimp chitin | 7.64 ± 0.06 | 1.59 ± 0.12 | 6.22 ± 0.06 |
2.2. Characterization of Chitin
2.2.1. IR Analysis
Assignments | Wave Number (cm−1) | |
---|---|---|
Beetle Chitin | Shrimp Chitin | |
ν (O-H) | 3,424 | 3,440 |
ν (N-H) | 3,262 | 3,268 |
ν (COCH3) | 2,934 | 2,932 |
ν (C-H) | 2,884 | 2,874 |
ν (C-O) | 1,655 | 1,654 |
ν (C=O of N-acetyl group) | 1,624 | 1,627 |
δ (N-H of N-acetyl group) | 1,560 | 1,560 |
2.2.2. Elemental Analysis
Samples | Content (%) | C/N | ||
---|---|---|---|---|
N | C | H | ||
Shrimp chitin | 6.24 | 43.75 | 6.40 | 7.07 |
Insect chitin | 6.45 | 44.36 | 5.92 | 6.88 |
2.2.3. XRD Analysis
2.2.4. Scanning Electron Microscopy (SEM)
3. Experimental
3.1. Isolation of Chitin from Adult H. parallela
3.2. Infrared Spectra (IR) Analysis
3.3. Elemental Analysis
3.4. X-ray Chitin Powder Diffraction
3.5. Scanning Electron Microscopy (SEM)
3.6. Composition Analysis
4. Conclusions
Acknowledgments
References and Notes
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Liu, S.; Sun, J.; Yu, L.; Zhang, C.; Bi, J.; Zhu, F.; Qu, M.; Jiang, C.; Yang, Q. Extraction and Characterization of Chitin from the Beetle Holotrichia parallela Motschulsky. Molecules 2012, 17, 4604-4611. https://doi.org/10.3390/molecules17044604
Liu S, Sun J, Yu L, Zhang C, Bi J, Zhu F, Qu M, Jiang C, Yang Q. Extraction and Characterization of Chitin from the Beetle Holotrichia parallela Motschulsky. Molecules. 2012; 17(4):4604-4611. https://doi.org/10.3390/molecules17044604
Chicago/Turabian StyleLiu, Shaofang, Jie Sun, Lina Yu, Chushu Zhang, Jie Bi, Feng Zhu, Mingjing Qu, Chen Jiang, and Qingli Yang. 2012. "Extraction and Characterization of Chitin from the Beetle Holotrichia parallela Motschulsky" Molecules 17, no. 4: 4604-4611. https://doi.org/10.3390/molecules17044604