Antioxidant and Antimicrobial Activities of Ethanol Extract from the Stem and Leaf of Impatiens balsamina L. (Balsaminaceae) at Different Harvest Times
Abstract
:1. Introduction
2. Results and Discussion
2.1. Total Phenolic and Flavonoid Content
Samples 1) | TPC (mg CE/g extract) | Flavonoid (mg QE/g extract) |
---|---|---|
S1 | 18.95 ± 3.08 c | 6.38 ± 1.60 e |
S2 | 17.02 ± 3.31 c | 8.13 ± 1.62 d |
S3 | 12.92 ± 3.25 d | 4.28 ± 2.16 f |
L1 | 79.55 ± 4.75 b | 57.43 ± 2.58 c |
L2 | 80.76 ± 5.32 b | 67.22 ± 2.16 b |
L3 | 103.94 ± 5.83 a | 104.28 ± 3.60 a |
2.2. Free Radical Scavenging Activity
2.3. Hydroxyl Radical Scavenging Activity
2.4. Antimicrobial Activity
Microorganism 1) | S1 | S2 | S3 | L1 | L2 | L3 |
---|---|---|---|---|---|---|
STA | -2) | - | - | ~ | + | ++ |
VIP | - | - | - | ++ | ++ | ++ |
BAC | - | - | - | ++ | ++ | ++ |
SAT | - | - | - | + | + | + |
LIM | + | - | - | ~ | ~ | + |
ECO | - | - | +++ | + | + | + |
CAN | + | - | ++ | +++ | +++ | +++ |
CLB | - | - | - | +++ | +++ | +++ |
3. Experimental
3.1. Chemicals and Extracts
3.2. Plant Samples
3.3. Extraction of Samples
3.4. Measurement of Total Phenolic Content Using Folin-Ciocalteu Assay
3.5. Measurement of Total Flavonoids
3.6. Measurement of Free Radical Scavenging Activity on DPPH Assay
3.7. Measurement of Hydroxyl Radical Scavenging Activity
3.8. Antimicrobial Activity
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Kang, S.-N.; Goo, Y.-M.; Yang, M.-R.; Ibrahim, R.I.H.; Cho, J.-H.; Kim, I.-S.; Lee, O.-H. Antioxidant and Antimicrobial Activities of Ethanol Extract from the Stem and Leaf of Impatiens balsamina L. (Balsaminaceae) at Different Harvest Times. Molecules 2013, 18, 6356-6365. https://doi.org/10.3390/molecules18066356
Kang S-N, Goo Y-M, Yang M-R, Ibrahim RIH, Cho J-H, Kim I-S, Lee O-H. Antioxidant and Antimicrobial Activities of Ethanol Extract from the Stem and Leaf of Impatiens balsamina L. (Balsaminaceae) at Different Harvest Times. Molecules. 2013; 18(6):6356-6365. https://doi.org/10.3390/molecules18066356
Chicago/Turabian StyleKang, Suk-Nam, Young-Min Goo, Mi-Ra Yang, Rashid Ismael Hag Ibrahim, Jae-Hyeon Cho, Il-Suk Kim, and Ok-Hwan Lee. 2013. "Antioxidant and Antimicrobial Activities of Ethanol Extract from the Stem and Leaf of Impatiens balsamina L. (Balsaminaceae) at Different Harvest Times" Molecules 18, no. 6: 6356-6365. https://doi.org/10.3390/molecules18066356